Lurcher mutant mice represent a natural model of genetically-determined olivocerebellar degeneration caused by a mutation in the δ2 glutamate receptor gene. They suffer from progressive postnatal loss of cerebellar Purkinje cells and a decrease of granule cells and inferior olive neurons. Their wild type littermates serve as healthy controls. A confocal laser scanning microscope was used aiming investigation the dynamics of changes in the cerebellar cortex of Lurcher and wild type mice derived from two strains during the period of 8-21 postnatal days. Fluorescent double-staining was used to visualize mainly the Purkinje cells in cerebellar slices. In wild types, only normal Purkinje cells of round or regular drop-shaped were present, when staining intensity of other individual cell structures differed in dependence on the age of the animal. In Lurcher mutants, there were still some normal-shaped cells. Nevertheless, depending on the animal's age, a wide variety of stages of the cell degeneration were depicted. The main characteristics of Purkinje cell degeneration in the early stage are: disruption of the continuity of the Purkinje cell layer, dark spots in cell nuclei and an irregular coloring of the cytoplasm. Later, the cells and their nuclei were deformed, often with two main dendrites sprouting from the cell body. Finally, the cell and nucleus margins were unclear, dendrites were significantly thickened, showing signs of shrinkage and fragmentation. Cell nucleoli underwent changes in number and appearance. No differences between the Lurcher mice of both strains (C3H and B6CBA) under examination were found.
- MeSH
- barvení a značení metody MeSH
- časové faktory MeSH
- fluorescenční barviva metabolismus MeSH
- glutamátové receptory nedostatek MeSH
- konfokální mikroskopie MeSH
- kůra mozečku patologie MeSH
- myši - mutanty neurologické MeSH
- myši MeSH
- Purkyňovy buňky chemie cytologie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- algoritmy MeSH
- dendrity fyziologie MeSH
- fyziologie buňky MeSH
- gating iontového kanálu fyziologie MeSH
- kůra mozečku anatomie a histologie fyziologie MeSH
- lidé MeSH
- membránové potenciály fyziologie MeSH
- mozková kůra anatomie a histologie fyziologie MeSH
- Purkyňovy buňky fyziologie chemie MeSH
- statistika jako téma metody MeSH
- teoretické modely MeSH
- Check Tag
- lidé MeSH
- MeSH
- časové faktory MeSH
- hipokampus chemie MeSH
- hypothalamus chemie MeSH
- injekce intraperitoneální MeSH
- interneurony chemie MeSH
- klasické podmiňování účinky léků MeSH
- krysa rodu rattus MeSH
- mozková kůra chemie MeSH
- neuroglie chemie MeSH
- neurony chemie metabolismus MeSH
- Purkyňovy buňky chemie MeSH
- RNA analýza aplikace a dávkování farmakologie metabolismus MeSH
- věkové faktory MeSH
- vyšší nervová činnost účinky léků MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- MeSH
- časové faktory MeSH
- hipokampus chemie MeSH
- hypothalamus chemie MeSH
- injekce intraperitoneální MeSH
- klasické podmiňování účinky léků MeSH
- krysa rodu rattus MeSH
- kůra mozečku chemie MeSH
- odstavení MeSH
- Purkyňovy buňky chemie MeSH
- RNA analýza farmakologie metabolismus MeSH
- věkové faktory MeSH
- vyšší nervová činnost účinky léků MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
