RNA transcript levels in the syphilis spirochete Treponema pallidum subsp. pallidum (Nichols) isolated from experimentally infected rabbits were determined by the use of DNA microarray technology. This characterization of the T. pallidum transcriptome during experimental infection provides further insight into the importance of gene expression levels for the survival and pathogenesis of this bacterium.

• MeSH
• bakteriální RNA MeSH
• exprese genu MeSH
• králíci MeSH
• messenger RNA MeSH
• otevřené čtecí rámce MeSH
• sekvenční analýza hybridizací s uspořádaným souborem oligonukleotidů MeSH
• stanovení celkové genové exprese metody   MeSH
• syfilis mikrobiologie   MeSH
• Treponema pallidum genetika   metabolismus   patogenita   MeSH
• zvířata MeSH
• Check Tag
• králíci MeSH
• zvířata MeSH
• Publikační typ
• práce podpořená grantem MeSH
• Research Support, U.S. Gov't, P.H.S. MeSH

We present the complete 2,843,201-bp genome sequence of Treponema denticola (ATCC 35405) an oral spirochete associated with periodontal disease. Analysis of the T. denticola genome reveals factors mediating coaggregation, cell signaling, stress protection, and other competitive and cooperative measures, consistent with its pathogenic nature and lifestyle within the mixed-species environment of subgingival dental plaque. Comparisons with previously sequenced spirochete genomes revealed specific factors contributing to differences and similarities in spirochete physiology as well as pathogenic potential. The T. denticola genome is considerably larger in size than the genome of the related syphilis-causing spirochete Treponema pallidum. The differences in gene content appear to be attributable to a combination of three phenomena: genome reduction, lineage-specific expansions, and horizontal gene transfer. Genes lost due to reductive evolution appear to be largely involved in metabolism and transport, whereas some of the genes that have arisen due to lineage-specific expansions are implicated in various pathogenic interactions, and genes acquired via horizontal gene transfer are largely phage-related or of unknown function.

• MeSH
• ABC transportéry genetika   MeSH
• bakteriální geny genetika   MeSH
• bakteriální proteiny genetika   MeSH
• Borrelia burgdorferi genetika   metabolismus   MeSH
• genom bakteriální * MeSH
• Leptospira interrogans genetika   metabolismus   MeSH
• modely genetické MeSH
• molekulární sekvence - údaje MeSH
• sekvence nukleotidů MeSH
• sekvenční homologie aminokyselin MeSH
• Treponema pallidum genetika   metabolismus   MeSH
• Treponema * genetika   metabolismus   patogenita   MeSH
• ústa * mikrobiologie   MeSH
• Publikační typ
• Research Support, U.S. Gov't, P.H.S. MeSH
• srovnávací studie MeSH