As opposed to pathogens passively circulating in the body fluids of their host, pathogenic species within the Spirochetes phylum are able to actively coordinate their movement in the host to cause systemic infections. Based on the unique morphology and high motility of spirochetes, we hypothesized that their surface adhesive molecules might be suitably adapted to aid in their dissemination strategies. Designing a system that mimics natural environmental signals, which many spirochetes face during their infectious cycle, we observed that a subset of their surface proteins, particularly Decorin binding protein (Dbp) A/B, can strongly enhance the motility of spirochetes in the extracellular matrix of the host. Using single-molecule force spectroscopy, we disentangled the mechanistic details of DbpA/B and decorin/laminin interactions. Our results show that spirochetes are able to leverage a wide variety of adhesion strategies through force-tuning transient molecular binding to extracellular matrix components, which concertedly enhance spirochetal dissemination through the host.

• MeSH
• bakteriální adheze * MeSH
• bakteriální adheziny genetika   metabolismus   MeSH
• Borrelia burgdorferi genetika   metabolismus   patogenita   MeSH
• dekorin metabolismus   MeSH
• extracelulární matrix metabolismus   mikrobiologie   MeSH
• interakce hostitele a patogenu MeSH
• kinetika MeSH
• klíště mikrobiologie   MeSH
• králíci MeSH
• laminin metabolismus   MeSH
• lymeská nemoc metabolismus   mikrobiologie   MeSH
• pohyb MeSH
• vazba proteinů MeSH
• zobrazení jednotlivé molekuly MeSH
• zvířata MeSH
• Check Tag
• králíci MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Borrelia burgdorferi sensu lato (Spirochaetes) is a group of at least 12 closely related species, some of which are responsible for chronic zoonotic infection that may cause Lyme disease. The only experimentally confirmed vector transmitting Borrelia to mammals is the Ixodes ticks. Borrelia is a highly adapted pathogen that can survive in the host organism in spite of the intense immune responses. Some patients have chronic long-lasting complications despite antibiotic therapy, probably due to adverse effects of the immune responses. A preventive vaccine against this bacterium has not been available due to the relatively broad spectrum and antigenic variability of Borrelia-surface lipoproteins and the different epitope recognition by experimental animals and humans. Although a human vaccine was marketed in the USA, it has been already pulled off the market. In addition, this vaccine was effective only in the USA, where the only pathogenic species is B. burgdorferi sensu stricto. Recent data indicate that a broadly effective vaccine will to be composed of a mixture of several antigens or multiple epitopes.

• MeSH
• antigenní variace MeSH
• antigeny bakteriální imunologie   metabolismus   MeSH
• antigeny povrchové imunologie   MeSH
• autoimunitní nemoci etiologie   MeSH
• bakteriální proteiny imunologie   metabolismus   MeSH
• bakteriální vakcíny imunologie   MeSH
• Borrelia burgdorferi imunologie   metabolismus   MeSH
• klíště imunologie   metabolismus   mikrobiologie   MeSH
• lidé MeSH
• lipoproteiny imunologie   metabolismus   MeSH
• lymeská nemoc imunologie   mikrobiologie   patologie   prevence a kontrola   MeSH
• molekulární sekvence - údaje MeSH
• myši MeSH
• proteiny vnější bakteriální membrány imunologie   MeSH
• protilátky bakteriální imunologie   MeSH
• vakcinace škodlivé účinky   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH
• Geografické názvy
• Spojené státy americké MeSH

We investigated the presence of glycoproteins in Borrelia burgdorferi. We did not find any evidence for glycosylation of the major outer membrane proteins OspA and OspB or the structural flagellar proteins FlaB and FlaA. We suggest that glycoproteins present on the surface of B. burgdorferi may be tightly bound culture medium glycoproteins.

• MeSH
• antigeny bakteriální genetika   metabolismus   MeSH
• antigeny povrchové genetika   metabolismus   MeSH
• bakteriální vakcíny genetika   metabolismus   MeSH
• Borrelia burgdorferi genetika   metabolismus   ultrastruktura   MeSH
• elektroforéza v polyakrylamidovém gelu MeSH
• elektronová kryomikroskopie MeSH
• financování organizované MeSH
• flagelin genetika   metabolismus   MeSH
• glykosylace MeSH
• hmotnostní spektrometrie s elektrosprejovou ionizací MeSH
• lipoproteiny genetika   metabolismus   MeSH
• molekulární sekvence - údaje MeSH
• proteiny vnější bakteriální membrány genetika   metabolismus   MeSH
• sekvence aminokyselin MeSH
• sekvenční homologie aminokyselin MeSH

• MeSH
• Borrelia burgdorferi metabolismus   MeSH
• finanční podpora výzkumu jako téma MeSH
• glykoproteiny analýza   chemie   MeSH
• lektiny analýza   metabolismus   MeSH
• oligosacharidy chemie   MeSH
• zmrazené řezy metody   využití   MeSH

We present the complete 2,843,201-bp genome sequence of Treponema denticola (ATCC 35405) an oral spirochete associated with periodontal disease. Analysis of the T. denticola genome reveals factors mediating coaggregation, cell signaling, stress protection, and other competitive and cooperative measures, consistent with its pathogenic nature and lifestyle within the mixed-species environment of subgingival dental plaque. Comparisons with previously sequenced spirochete genomes revealed specific factors contributing to differences and similarities in spirochete physiology as well as pathogenic potential. The T. denticola genome is considerably larger in size than the genome of the related syphilis-causing spirochete Treponema pallidum. The differences in gene content appear to be attributable to a combination of three phenomena: genome reduction, lineage-specific expansions, and horizontal gene transfer. Genes lost due to reductive evolution appear to be largely involved in metabolism and transport, whereas some of the genes that have arisen due to lineage-specific expansions are implicated in various pathogenic interactions, and genes acquired via horizontal gene transfer are largely phage-related or of unknown function.

• MeSH
• ABC transportéry genetika   MeSH
• bakteriální geny genetika   MeSH
• bakteriální proteiny genetika   MeSH
• Borrelia burgdorferi genetika   metabolismus   MeSH
• genom bakteriální * MeSH
• Leptospira interrogans genetika   metabolismus   MeSH
• modely genetické MeSH
• molekulární sekvence - údaje MeSH
• sekvence nukleotidů MeSH
• sekvenční homologie aminokyselin MeSH
• Treponema pallidum genetika   metabolismus   MeSH
• Treponema * genetika   metabolismus   patogenita   MeSH
• ústa * mikrobiologie   MeSH
• Publikační typ
• Research Support, U.S. Gov't, P.H.S. MeSH
• srovnávací studie MeSH