The aim of this study was to evaluate the dynamics of the cytotoxicity of gingival margin retraction astringents based on aluminium chloride, aluminium sulphate, and ferric sulphate (solutions and gels) in human fibroblasts isolated from gingiva. The cytocompatibility of ten astringent-based chemical retraction agents: Gingiva Liquid, Alustin, Racestypine, Orbat sensitive, Astringedent®, Alustat, Hemostat, Racécord, Gel cord and ViscoStat®, in dilutions of 1 : 10 and 1 : 20, with human gingival fibroblasts was investigated. The MTT assay was performed to determine oxidoreductive mitochondrial function after 3, 5, 10 min and 24 h of incubation. Cell viability was determined according to the chemical group, concentration, exposure time, and the clinical form of the gingival retraction agents. Ferric sulphate- based agents were the most cytotoxic, followed by aluminium chloride and aluminium sulphate. The form of the astrigents influenced cell viability. The evaluated astringents may have cytotoxic potential for gingival margin tissues under clinical conditions.
- MeSH
- adstringencia toxicita MeSH
- barvicí látky diagnostické užití MeSH
- chloridy toxicita MeSH
- fibroblasty účinky léků MeSH
- gingiva cytologie účinky léků MeSH
- kamencové sloučeniny toxicita MeSH
- kultivované buňky MeSH
- lidé MeSH
- oxidace-redukce účinky léků MeSH
- sloučeniny hliníku toxicita MeSH
- tetrazoliové soli diagnostické užití MeSH
- thiazoly diagnostické užití MeSH
- viabilita buněk účinky léků MeSH
- železité sloučeniny toxicita MeSH
- Check Tag
- lidé MeSH
