The epithelial-mesenchymal plasticity, in tight association with stemness, contributes to the mammary gland homeostasis, evolution of early neoplastic lesions and cancer dissemination. Focused on cell surfaceome, we used mouse models of pre-neoplastic mammary epithelial and cancer stem cells to reveal the connection between cell surface markers and distinct cell phenotypes. We mechanistically dissected the TGF-β family-driven regulation of Sca-1, one of the most commonly used adult stem cell markers. We further provided evidence that TGF-β disrupts the lineage commitment and promotes the accumulation of tumor-initiating cells in pre-neoplastic cells.
- MeSH
- ataxin-1 metabolismus MeSH
- epitelo-mezenchymální tranzice genetika MeSH
- epitelové buňky patologie MeSH
- experimentální nádory mléčných žláz genetika patologie MeSH
- lidé MeSH
- mléčné žlázy zvířat patologie MeSH
- myši MeSH
- nádorové buněčné linie transplantace MeSH
- nádorové kmenové buňky patologie MeSH
- nádory prsu genetika patologie MeSH
- plasticita buňky genetika MeSH
- receptor erbB-2 genetika MeSH
- regulace genové exprese u nádorů MeSH
- rekombinantní proteiny genetika metabolismus MeSH
- signální transdukce genetika MeSH
- transformující růstový faktor beta genetika metabolismus MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Spinocerebellar ataxia type-1 (SCA1) is caused by an abnormally expanded polyglutamine (polyQ) tract in ataxin-1. These expansions are responsible for protein misfolding and self-assembly into intranuclear inclusion bodies (IIBs) that are somehow linked to neuronal death. However, owing to lack of a suitable cellular model, the downstream consequences of IIB formation are yet to be resolved. Here, we describe a nuclear protein aggregation model of pathogenic human ataxin-1 and characterize IIB effects. Using an inducible Sleeping Beauty transposon system, we overexpressed the ATXN1(Q82) gene in human mesenchymal stem cells that are resistant to the early cytotoxic effects caused by the expression of the mutant protein. We characterized the structure and the protein composition of insoluble polyQ IIBs which gradually occupy the nuclei and are responsible for the generation of reactive oxygen species. In response to their formation, our transcriptome analysis reveals a cerebellum-specific perturbed protein interaction network, primarily affecting protein synthesis. We propose that insoluble polyQ IIBs cause oxidative and nucleolar stress and affect the assembly of the ribosome by capturing or down-regulating essential components. The inducible cell system can be utilized to decipher the cellular consequences of polyQ protein aggregation. Our strategy provides a broadly applicable methodology for studying polyQ diseases.
- MeSH
- ataxin-1 genetika metabolismus MeSH
- intranukleární inkluzní tělíska * metabolismus MeSH
- jaderné proteiny genetika metabolismus MeSH
- lidé MeSH
- oxidační stres MeSH
- proteiny nervové tkáně * genetika metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH