The minipig provides an excellent experimental model for tooth morphogenesis because its diphyodont and heterodont dentition resemble that of humans. However, little information is available on the processes of tooth development in the pig. The purpose of this study was to classify the early stages of odontogenesis in minipigs from the initiation of deciduous dentition to the late bell stage when the successional dental lamina begins to develop. To analyze the initiation of teeth anlagens and the structural changes of dental lamina, a three-dimensional (3D) analysis was performed. At the earliest stage, 3D reconstruction revealed a continuous dental lamina along the length of the jaw. Later, the dental lamina exhibited remarkable differences in depth, and the interdental lamina was shorter. The dental lamina grew into the mesenchyme in the lingual direction, and its inclined growth was underlined by asymmetrical cell proliferation. After the primary tooth germ reached the late bell stage, the dental lamina began to disintegrate and fragmentize. Some cells disappeared during the process of lamina degradation, while others remained in small islands known as epithelial pearls. The minipig can therefore, inter alia, be used as a model organism to study the fate of epithelial pearls from their initiation to their contribution to pathological structures, primarily because of the clinical significance of these epithelial rests.
- MeSH
- bazální membrána embryologie MeSH
- buněčná diferenciace fyziologie MeSH
- dentin embryologie MeSH
- epitel embryologie MeSH
- mezoderm embryologie MeSH
- miniaturní prasata MeSH
- modely u zvířat MeSH
- morfogeneze fyziologie MeSH
- odontoblasty cytologie MeSH
- odontogeneze fyziologie MeSH
- orgán skloviny embryologie MeSH
- počítačové zpracování obrazu metody MeSH
- prasata MeSH
- premolár embryologie MeSH
- proliferace buněk MeSH
- proliferační antigen buněčného jádra analýza MeSH
- řezáky embryologie MeSH
- špičák embryologie MeSH
- zobrazování trojrozměrné metody MeSH
- zubní zárodek embryologie MeSH
- zuby mléčné embryologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
Tooth number is abnormal in about 20% of the human population. The most common defect is agenesis of the third molars, followed by loss of the lateral incisors and loss of the second premolars. Tooth loss appears as both a feature of multi-organ syndromes and as a non-syndromic isolated character. Apart from tooth number, abnormalities are also observed in tooth size, shape, and structure. Many of the genes that underlie dental defects have been identified, and several mouse models have been created to allow functional studies to understand, in greater detail, the role of particular genes in tooth development. The ability to manipulate the mouse embryo using explant culture and genome targeting provides a wealth of information that ultimately may pave the way for better diagnostics, treatment or even cures for human dental disorders. This review aims to summarize recent knowledge obtained in mouse models, which can be used to gain a better understanding of the molecular basis of human dental abnormalities.
- MeSH
- abnormality zubů embryologie genetika patologie MeSH
- anodoncie genetika MeSH
- dentin abnormality embryologie MeSH
- fenotyp MeSH
- financování organizované MeSH
- fosfoproteiny genetika MeSH
- kostní morfogenetické proteiny genetika MeSH
- kostní morfogenetický protein 4 MeSH
- lidé MeSH
- modely u zvířat MeSH
- myši MeSH
- odontogeneze genetika MeSH
- parodont abnormality MeSH
- zubní sklovina abnormality embryologie MeSH
- zuby přespočetné embryologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- přehledy MeSH
