Many enzymes from the short-chain dehydrogenase/reductase superfamily (SDR) have already been well characterized, particularly those that participate in crucial biochemical reactions in the human body (e.g. 11β-hydroxysteroid dehydrogenase 1, 17β-hydroxysteroid dehydrogenase 1 or carbonyl reductase 1). Several other SDR enzymes are completely or almost completely uncharacterized, such as DHRS1 (also known as SDR19C1). Based on our in silico and experimental approaches, DHRS1 is described as a likely monotopic protein that interacts with the membrane of the endoplasmic reticulum. The highest expression level of DHRS1 protein was observed in human liver and adrenals. The recombinant form of DHRS1 was purified using the detergent n-dodecyl-β-D-maltoside, and DHRS1 was proven to be an NADPH-dependent reductase that is able to catalyse the in vitro reductive conversion of some steroids (estrone, androstene-3,17-dione and cortisone), as well as other endogenous substances and xenobiotics. The expression pattern and enzyme activities fit to a role in steroid and/or xenobiotic metabolism; however, more research is needed to fully clarify the exact biological function of DHRS1.

• MeSH
• dehydrogenasy/reduktasy s krátkým řetězcem metabolismus   MeSH
• endoplazmatické retikulum metabolismus   MeSH
• estron metabolismus   MeSH
• HeLa buňky MeSH
• játra metabolismus   MeSH
• kortison metabolismus   MeSH
• lidé MeSH
• nadledviny metabolismus   MeSH
• nádorové buněčné linie MeSH
• oxidoreduktasy genetika   metabolismus   MeSH
• rekombinantní proteiny biosyntéza   genetika   MeSH
• sekvence aminokyselin MeSH
• Sf9 buňky MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

The aim of the present research was to study the uptake of DHEAS, and to establish the intracrine capacity of human platelets to produce sex steroid hormones. The DHEAS transport was evaluated through the uptake of [(3)H]-DHEAS in the presence or absence of different substrates through the organic anion transporting polypeptide (OATP) family. The activity of sulfatase enzyme was evaluated, and the metabolism of DHEAS was measured by the conversion of [(3)H]-DHEAS to [(3)H]-androstenedione, [(3)H]-testosterone, [(3)H]-estrone and [(3)H]-17beta-estradiol. Results indicated the existence in the plasma membrane of an OATP with high affinity for DHEAS and estrone sulphate (E(1)S). The platelets showed the capacity to convert DHEAS to active DHEA by the steroid-sulfatase activity. The cells resulted to be a potential site for androgens production, since they have the capacity to produce androstenedione and testosterone; in addition, they reduced [(3)H]-estrone to [(3)H]-17beta-estradiol. This is the first demonstration that human platelets are able to import DHEAS and E(1)S using the OATP family and to convert DHEAS to active DHEA, and to transform E(1)S to 17beta-estradiol.

• MeSH
• androgeny metabolismus   MeSH
• androstendion metabolismus   MeSH
• dehydroepiandrosteron metabolismus   MeSH
• dehydroepiandrosteronsulfát metabolismus   MeSH
• estradiol metabolismus   MeSH
• estron analogy a deriváty   metabolismus   MeSH
• lidé MeSH
• přenašeče organických aniontů metabolismus   MeSH
• testosteron metabolismus   MeSH
• trombocyty chemie   metabolismus   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

• MeSH
• estradiol analýza   krev   metabolismus   MeSH
• estrogenní substituční terapie MeSH
• estrogeny analýza   krev   metabolismus   MeSH
• estron analýza   krev   metabolismus   MeSH
• lidé MeSH
• menopauza fyziologie   krev   metabolismus   MeSH
• osteokalcin účinky léků   krev   metabolismus   MeSH
• postmenopauzální osteoporóza MeSH
• Check Tag
• lidé MeSH
• ženské pohlaví MeSH

• MeSH
• androstendion metabolismus   MeSH
• estron metabolismus   MeSH
• játra metabolismus   MeSH
• ledviny metabolismus   MeSH
• lidé MeSH
• lidské chromozomy, 16-18 MeSH
• mozek metabolismus   MeSH
• nadledviny metabolismus   MeSH
• oxidace-redukce MeSH
• plíce metabolismus   MeSH
• plod metabolismus   MeSH
• pohlavní steroidní hormony metabolismus   MeSH
• pregnenolon metabolismus   MeSH
• progesteron metabolismus   MeSH
• progestiny metabolismus   MeSH
• těhotenství MeSH
• testis metabolismus   MeSH
• testosteron metabolismus   MeSH
• thymus metabolismus   MeSH
• trizomie MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• těhotenství MeSH
• ženské pohlaví MeSH