Analytical devices that combine sensitive biological component with a physicochemical detector hold a great potential for various applications, e.g., environmental monitoring, food analysis or medical diagnostics. Continuous efforts to develop inexpensive sensitive biodevices for detecting target substances typically focus on the design of biorecognition elements and their physical implementation, while the methods for processing signals generated by such devices have received far less attention. Here, we present fundamental considerations related to signal processing in biosensor design and investigate how undemanding signal treatment facilitates calibration and operation of enzyme-based biodevices. Our signal treatment approach was thoroughly validated with two model systems: (i) a biodevice for detecting chemical warfare agents and environmental pollutants based on the activity of haloalkane dehalogenase, with the sensitive range for bis(2-chloroethyl) ether of 0.01-0.8 mM and (ii) a biodevice for detecting hazardous pesticides based on the activity of γ-hexachlorocyclohexane dehydrochlorinase with the sensitive range for γ-hexachlorocyclohexane of 0.01-0.3 mM. We demonstrate that the advanced signal processing based on curve fitting enables precise quantification of parameters important for sensitive operation of enzyme-based biodevices, including: (i) automated exclusion of signal regions with substantial noise, (ii) derivation of calibration curves with significantly reduced error, (iii) shortening of the detection time, and (iv) reliable extrapolation of the signal to the initial conditions. The presented simple signal curve fitting supports rational design of optimal system setup by explicit and flexible quantification of its properties and will find a broad use in the development of sensitive and robust biodevices.
- MeSH
- biosenzitivní techniky metody MeSH
- chemické bojové látky analýza MeSH
- chlorované uhlovodíky analýza MeSH
- enzymy metabolismus MeSH
- ether analogy a deriváty analýza MeSH
- hexany analýza MeSH
- hydrolasy metabolismus MeSH
- kalibrace MeSH
- látky znečišťující životní prostředí analýza MeSH
- lyasy metabolismus MeSH
- počítačové zpracování signálu * MeSH
- senzitivita a specificita MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- amfetamin * izolace a purifikace metabolismus škodlivé účinky MeSH
- doping ve sportu * dějiny metody prevence a kontrola MeSH
- ether analogy a deriváty izolace a purifikace metabolismus MeSH
- lidé MeSH
- nemocnice fakultní metody organizace a řízení využití MeSH
- preventivní lékařství * metody organizace a řízení trendy MeSH
- statistika jako téma MeSH
- stimulancia izolace a purifikace metabolismus škodlivé účinky MeSH
- strychnin izolace a purifikace metabolismus škodlivé účinky MeSH
- Check Tag
- lidé MeSH
- Geografické názvy
- Československo MeSH
