Adequate treatment of microbial infections requires rapid and accurate identification of the etiological agent. In routine diagnostics, identification of bacteria conventionally relies on phenotypic testing, which can be hindered by phenotypic variations. Therefore, genotyping techniques should perform faster and more accurately. Recently, the technique of high-resolution melting analysis (HRMA) of PCR amplicons promises to provide a convenient and economic tool of genotypic identification. In our study, we performed prospective routine testing of a PCR-HRMA system that was recently published in a proof-of-the-principle study. The system was evaluated by analysing 275 clinical isolates of bacteria acquired from 65 patients suffering from cystic fibrosis or chronic obstructive pulmonary disease. Our results show that its routine use may result in partial worsening of its discriminatory power; however, it still outmatched conventional phenotyping in the group of non-fermentative Gram-negative rods. Moreover, when supplemented by rapid, simple and economic oxidase test, it can be even simplified for more economic performance.
- MeSH
- bakteriologické techniky metody MeSH
- chronická obstrukční plicní nemoc komplikace MeSH
- cystická fibróza komplikace MeSH
- diagnostické techniky molekulární metody MeSH
- DNA bakterií chemie genetika MeSH
- gramnegativní aerobní tyčinky a koky genetika izolace a purifikace MeSH
- gramnegativní bakteriální infekce diagnóza mikrobiologie MeSH
- infekce dýchací soustavy mikrobiologie MeSH
- lidé MeSH
- polymerázová řetězová reakce metody MeSH
- prospektivní studie MeSH
- tranzitní teplota MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
