Cílem práce je vyhodnocení výsledků MESA/TESE (MESA – microsurgical sperm aspiration, TESE – testicular sperm extraction) u pacientů s azoospermií v průběhu devíti let spolupráce Urologické kliniky a Centra asistované reprodukce FN Brno a dopad na gravidity, kterých jsme dosáhli. Celkem bylo provedeno 70 operačních výkonů. Výsledkem bylo dosaženo 17 gravidit vlastními spermiemi, z toho byly dva aborty, dále osm pacientů bez embryotransferu.
The objective of this study is to evaluate the results of microsurgical sperm aspiration and testicular sperm extraction in patients with azoospermia during nine years of cooperation of Urological Department and Center for Assisted Reproduction University Hospital Brno and its impact on the obtained gravidity. In total 70 surgical interventions were performed, which resulted in 17 cases of gravidity using own sperm, including two abortions, and eight patients without embryo transfer.
- Klíčová slova
- mikrochirurgická aspirace spermií, extrakce spermií z varlete,
- MeSH
- azoospermie * diagnóza terapie MeSH
- dospělí MeSH
- fertilizace in vitro * metody statistika a číselné údaje MeSH
- intracytoplazmatické injekce spermie metody statistika a číselné údaje MeSH
- lidé středního věku MeSH
- lidé MeSH
- odběr spermií * mortalita MeSH
- retrospektivní studie MeSH
- těhotenství statistika a číselné údaje MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- těhotenství statistika a číselné údaje MeSH
- ženské pohlaví MeSH
OBJECTIVE: To determine whether varied human spermatozoa, as detected with monoclonal antibodies against acrosomal proteins, have an influence on fertilization, transfer, pregnancy, and implantation rates when intracytoplasmic sperm injection is used. DESIGN: A retrospective study. SETTING: A private IVF center and academic research laboratory. PATIENT(S): One thousand two hundred forty men participating in the intracytoplasmic sperm injection program. INTERVENTION(S): Sperm were divided into seven groups: oligozoospermia, oligoasthenozoospermia, and oligoasthenoteratozoospermia and fresh and frozen-thawed epididymal and fresh and frozen-thawed testicular sperm. Fertilization, transfer, pregnancy, and implantation rates were recorded in each category. Sperm were tested with antibodies for detection of the of the sperm acrosome. MAIN OUTCOME MEASURE(S): Fertilization, transfer, pregnancy and implantation rates, and percentage of acrosome-reacted cells. RESULT(S): The fertilization rate and statistical evaluation showed differences between morphologically normal and pathological sperm and other groups. The freezing-thawing procedure had no influence on the fertilization of testicular sperm, but epididymal frozen-thawed sperm had a higher fertilization rate. Immunofluorescence proved decreasing sperm quality in all groups compared with the control group. This difference is not manifested in other parameters (transfer, pregnancy, implantation rates). CONCLUSION(S): The spermatozoa with varied semen characteristics and good quality, also detected with specific antibodies, gave the best fertilization rates. The paternal effect is not proved in other parameters.
- MeSH
- akrozom imunologie MeSH
- financování organizované MeSH
- hodnocení výsledků zdravotní péče metody MeSH
- imunoanalýza metody MeSH
- imunokomplex analýza MeSH
- intracytoplazmatické injekce spermie statistika a číselné údaje MeSH
- kultivované buňky MeSH
- lidé MeSH
- mužská infertilita epidemiologie imunologie terapie MeSH
- retrospektivní studie MeSH
- sperma cytologie imunologie MeSH
- těhotenství MeSH
- úhrn těhotenství na počet žen v reprodukčním věku MeSH
- výsledek terapie MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
