Yeasts Cryptococcus humicola accumulated cadmium, cobalt, and iron (~ 50, 17, and 4% of the content in the medium, respectively) from the medium containing glucose, phosphate, and 2 mmol/L of metal salts. The effects of metal absorption on the levels of orthophosphate (Pi) and inorganic polyphosphate (polyP) varied for the metals under study. The levels of Pi and polyP increased in the case of cadmium and cobalt, respectively. In the case of iron, no changes in the levels of Pi and polyP were observed. Multiple DAPI-stained polyP inclusions were observed in the cytoplasm of cadmium-containing cells. The intensity of DAPI staining of the cell wall especially increased in case of cobalt and iron accumulation.

• MeSH
• biomasa MeSH
• Cryptococcus metabolismus   MeSH
• dusík metabolismus   MeSH
• kadmium chemie   metabolismus   farmakokinetika   MeSH
• kobalt chemie   metabolismus   farmakokinetika   MeSH
• polyfosfáty chemie   metabolismus   farmakokinetika   MeSH
• sorpční detoxikace MeSH
• železo chemie   metabolismus   farmakokinetika   MeSH
• Publikační typ
• časopisecké články MeSH

The extent of arsenic (As) and metal accumulation in fronds of the As hyperaccumulator Pteris vittata (Chinese brake fern) and in leaves of Cyperus involucratus, which grow on the surface of an old flotation tailings pond in the Zambian Copperbelt province, was studied. The tailings consist of two types of material with distinct chemical composition: (1) reddish-brown tailings rich in As, iron (Fe), and other metals, and (2) grey-green tailings with a lower content of As, Fe, and other metals, apart from manganese (Mn). P. vittata accumulates from 2350 to 5018 μg g(-1) As (total dry weight [dw]) in its fronds regardless of different total and plant-available As concentrations in both types of tailings. Concentrations of As in C. involucratus leaves are much lower (0.24-30.3 μg g(-1) dw). Contents of copper (Cu) and cobalt (Co) in fronds of P. vittata (151-237 and 18-38 μg g(-1) dw, respectively) and in leaves of C. involucratus (96-151 and 9-14 μg g(-1) dw, respectively) are high, whereas concentrations of other metals (Fe, Mn, and zinc [Zn]) are low and comparable with contents of the given metals in common plants. Despite great differences in metal concentrations in the two types of deposited materials, concentrations of most metals in plant tissues are very similar. This indicates an exclusion or avoidance mechanism operating when concentrations of the metals in substrate are particularly high. The results of the investigation show that Chinese brake fern is not only a hyperaccumulator of As but has adapted itself to high concentrations of Cu and Co in flotation tailings of the Zambian Copperbelt.

• MeSH
• arsen analýza   farmakokinetika   MeSH
• Cyperus metabolismus   MeSH
• kobalt analýza   farmakokinetika   MeSH
• kořeny rostlin metabolismus   MeSH
• látky znečišťující půdu analýza   MeSH
• listy rostlin metabolismus   MeSH
• měď analýza   farmakokinetika   MeSH
• monitorování životního prostředí metody   MeSH
• Pteris metabolismus   MeSH
• zinek analýza   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Zambie MeSH