The genome sequence of a mitovirus found in an isolate of Diaporthe rudis, one of the causal agents of Phomopsis dieback on grapevines, was determined by two high-throughput sequencing approaches, small RNA and total RNA sequencing. The genome of this mitovirus is 2,455 nt in length and includes a single large open reading frame (ORF) encoding an RNA-dependent RNA polymerase (RdRp). A BLASTx comparison of the full-length genome sequence showed the highest similarity (54.15%) with that of Colletotrichum falcatum mitovirus 1 (CfMV1). Our results reveal a new member of the genus Mitovirus first detected in D. rudis (Fr.) Nitschke, with the proposed name "Diaporthe rudis mitovirus 1" (DrMV1).
- MeSH
- délka genomu MeSH
- exprese genu MeSH
- fylogeneze * MeSH
- genom virový * MeSH
- mykoviry klasifikace genetika izolace a purifikace MeSH
- nemoci rostlin mikrobiologie MeSH
- otevřené čtecí rámce MeSH
- RNA-dependentní RNA-polymerasa genetika MeSH
- Saccharomycetales virologie MeSH
- sekvenování celého genomu MeSH
- virové proteiny genetika MeSH
- Vitis mikrobiologie MeSH
- Publikační typ
- časopisecké články MeSH
A lichen body is formed most often from green alga cells trapped in a net of ascomycetous fungi and accompanied by endolichenic or parasitic fungi, other algae, and symbiotic or free-living bacteria. The lichen's microcosmos is inhabited by mites, insects, and other animals for which the lichen is a source of food or a place to live. Novel, four-segmented dsRNA viruses were detected in saxicolous Chrysothrixchlorina and Leprariaincana lichens. Comparison of encoded genome proteins revealed classification of the viruses to the genus Alphachrysovirus and a relationship to chrysoviruses from filamentous ascomycetous fungi. We propose the names Chrysothrix chrysovirus 1 (CcCV1) and Lepraria chrysovirus 1 (LiCV1) as acronyms for these viruses. Surprisingly, observation of Chrysothrixchlorina hybridization with fluorescent-labelled virus probe by confocal microscope revealed that the CcCV1 virus is not present in the lichen body-forming fungus but in accompanying endolichenic Penicilliumcitreosulfuratum fungus. These are the first descriptions of mycoviruses from a lichen environment.
- MeSH
- dvouvláknová RNA MeSH
- fylogeneze MeSH
- genom virový MeSH
- genomika metody MeSH
- lišejníky virologie MeSH
- mykoviry klasifikace fyziologie MeSH
- RNA-viry MeSH
- sekvence aminokyselin MeSH
- symbióza * MeSH
- virové proteiny chemie genetika MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
A novel bisegmented double-stranded RNA virus was identified in the mycoparasitic and opportunistic fungus Hypomyces chrysospermus. The RNA1 genome segment comprises 1866 bp and encodes an RNA-dependent RNA polymerase (RdRp). The RNA2 segment comprises 1822 bp and encodes a capsid protein. Phylogenetic analysis of the RdRp protein indicated that this virus is a new member of genus Alphapartitivirus in the family Partitiviridae. We have designated this mycovirus as "Hypomyces chrysospermus partitivirus 1" (HcPV1). HcPV1 is highly transmissible with aleurioconidia and is present in large amounts within growing mycelium in comparison to the GAPDH reference gene.
- MeSH
- fylogeneze * MeSH
- genom virový MeSH
- Hypocreales virologie MeSH
- mykoviry klasifikace genetika izolace a purifikace MeSH
- RNA-dependentní RNA-polymerasa genetika MeSH
- RNA-viry klasifikace genetika izolace a purifikace MeSH
- sekvenční analýza DNA MeSH
- virové plášťové proteiny genetika MeSH
- Publikační typ
- časopisecké články MeSH
The European race of the ascomycetous species Gremmeniella abietina (Lagerberg) Morelet includes causal agents of shoot blight and stem canker of several conifers in Europe and North America, which are known to host a diverse virome. GaRV6 is the latest and sixth mycovirus species reported within G. abietina. Before its description, one victorivirus and one gammapartitivirus species were described in biotype A, two mitoviruses in both biotypes A and B and a betaendornavirus in biotype B. Possible phenotypic changes produced by mycoviruses on G. abietina mycelial growth have been reported in Spanish mitovirus-free and GaRV6-hosting G. abietina isolates, which had higher growth rates at the optimal temperature of 15 °C, but no other major differences have been observed between partitivirus-like dsRNA and dsRNA-free isolates. In this review, we reappraise the diversity of viruses found in G. abietina so far, and their relevance in clarifying the taxonomy of G. abietina. We also provide evidence for the presence of two new viruses belonging to the families Fusariviridae and Endornaviridae in Spanish isolates.
- MeSH
- Ascomycota virologie MeSH
- biodiverzita * MeSH
- cévnaté rostliny mikrobiologie MeSH
- mykoviry klasifikace izolace a purifikace MeSH
- nemoci rostlin mikrobiologie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
- Geografické názvy
- Evropa MeSH
- Severní Amerika MeSH
The complete nucleotide sequence of a new RNA mycovirus in the KY isolate of Phomopsis longicolla Hobbs 1985 and its protoplasts subcultures p5, p9, and ME711 was discovered. The virus, provisionally named Phomopsis longicolla RNA virus 1 (PlRV1), was localized in mitochondria and was determined to have a genome 2822 nucleotides long. A single open reading frame could be translated in silico by both standard and mitochondrial genetic codes into a product featuring conservative domains for an RNA-dependent RNA polymerase (RdRp). The RdRp of PlRV1 has no counterpart among mycoviruses, but it is about 30% identical with the RdRp of plant ourmiaviruses. Recently, new mycoviruses related to plant ourmiaviruses and forming one clade with PlRV1 have been discovered. This separate clade could represent the crucial link between plant and fungal viruses.
- MeSH
- Ascomycota virologie MeSH
- fylogeneze MeSH
- genom virový MeSH
- molekulární sekvence - údaje MeSH
- mykoviry klasifikace genetika izolace a purifikace MeSH
- nemoci rostlin virologie MeSH
- otevřené čtecí rámce MeSH
- RNA virová genetika MeSH
- RNA-viry klasifikace genetika izolace a purifikace MeSH
- rostlinné viry klasifikace genetika izolace a purifikace MeSH
- sekvence aminokyselin MeSH
- sekvence nukleotidů MeSH
- virové proteiny genetika MeSH
- Publikační typ
- časopisecké články MeSH
A novel dsRNA virus was identified in the mycorrhizal fungus Thelephora terrestris (Ehrh.) and sequenced. This virus, named Thelephora terrestris virus 1 (TtV1), contains two reading frames in different frames but with the possibility that ORF2 could be translated as a fusion polyprotein after ribosomal -1 frameshifting. Picornavirus 2A-like motif, nudix hydrolase, phytoreovirus S7, and RdRp domains were found in a unique arrangement on the polyprotein. A new genus named Phlegivirus and containing TtV1, PgLV1, RfV1 and LeV is therefore proposed. Twenty species of oribatid mites were identified in soil material in the vicinity of T. terrestris. TtV1 was detected in large amounts in Steganacarus (Tropacarus) carinatus (C.L. Koch, 1841) and in much smaller amounts in Nothrus silvestris (Nicolet). This is the first description of mycovirus presence in oribatid mites.
- MeSH
- Basidiomycota virologie MeSH
- Bunyaviridae klasifikace genetika izolace a purifikace MeSH
- fylogeneze MeSH
- molekulární sekvence - údaje MeSH
- mykorhiza klasifikace genetika izolace a purifikace MeSH
- mykoviry klasifikace genetika izolace a purifikace MeSH
- roztoči mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Purpureocillium lilacinum is a ubiquitous saprophytic fungus commonly isolated from soils and widely known as a biological control agent against phytopathogenic nematodes and pest insects. Mycoviruses infect a wide number of fungal species, but the study of viruses infecting entomopathogenic fungi is still quite recent. In this study, a total of 86 P. lilacinum isolates collected from soil in natural and cultivated habitats throughout the Czech Republic were analyzed; 22 % of the isolates harbored double-stranded RNA (dsRNA) elements with viral characteristics. These results suggest that mycoviruses are common in P. lilacinum. One of the most common dsRNA elements detected in the survey was completely sequenced and corresponded to the 2,864-bp genome of a previously undescribed mycovirus, designated Purpureocillium lilacinum nonsegmented virus 1 (PlNV-1). Phylogenetic analysis of the RNA-dependent RNA polymerase of PlNV-1 indicated that this virus might belong to a new taxon related to the family Partitiviridae.
- MeSH
- dvouvláknová RNA genetika MeSH
- fylogeneze MeSH
- genom virový MeSH
- Hypocreales izolace a purifikace virologie MeSH
- mykoviry klasifikace genetika izolace a purifikace MeSH
- půdní mikrobiologie MeSH
- RNA virová genetika MeSH
- RNA-viry klasifikace genetika izolace a purifikace MeSH
- sekvenční analýza DNA MeSH
- sekvenční homologie MeSH
- shluková analýza MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
