To explore regulation and function of cyclin D2, a candidate cell cycle-regulatory proto-oncogene, we examined subcellular localisation, cell type- and cell cycle-dependent expression, and requirement of cyclin D2 protein for G1 progression, in a panel of 40 human normal and cancer cell types. Except for lymphoid cells and sarcoma cell lines, expression of cyclin D2 was considerably more restricted than that of cyclin D1, whereas both D-type cyclin proteins were low or undetectable in cells lacking functional retinoblastoma gene product. In G1 cells, the cyclin D2 protein was more resistant to extraction and localised predominantly to nuclei, whereas it became more soluble and distributed in both nuclei and cytoplasm from G1/S transition onwards. Centrifugal elutriation and multiparameter flow cytometry analyses of several cell types showed moderate cell cycle oscillation with maximum levels of the cyclin D2 protein reached in late G1. Microinjection and/or electroporation of antibodies to cyclin D2 during G1 arrested the cyclin D2-expressing lymphocytes, breast myoepithelium, and U-2-OS sarcoma cells in G1 phase, whereas cyclin D2-negative cell types were unaffected by such treatment. Consistent with the putative proto-oncogenic role of cyclin D2 in specific cell types, our data show that this G1 cyclin has properties closely resembling those of cyclin D1, including the essential positive role in regulation of G1.
- MeSH
- buněčné linie MeSH
- cyklin D1 MeSH
- cyklin D2 MeSH
- cykliny fyziologie imunologie MeSH
- G1 fáze * fyziologie MeSH
- lidé MeSH
- monoklonální protilátky imunologie MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- nádorové buňky kultivované MeSH
- nukleoproteiny fyziologie imunologie MeSH
- onkogenní proteiny fyziologie MeSH
- subcelulární frakce metabolismus MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
A comparative study of influenza A (H1N1) virus strains isolated in one epidemic season (1988-1989) in neighbouring countries was carried out. Using polyclonal and monoclonal antibodies, some of the isolates were shown to have different antigenic and biological properties which probably determined the different intensity of the epidemic situation in the USSR and CzSFR in 1988-1989.
- MeSH
- antigeny virové krev MeSH
- chřipka lidská imunologie mikrobiologie MeSH
- epidemický výskyt choroby MeSH
- hemaglutininy virové imunologie MeSH
- lidé MeSH
- neuraminidasa imunologie MeSH
- nukleoproteiny imunologie MeSH
- virové proteiny imunologie MeSH
- virus chřipky A, podtyp H1N1 * MeSH
- virus chřipky A enzymologie imunologie izolace a purifikace MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- srovnávací studie MeSH
- Geografické názvy
- Československo MeSH
- SSSR MeSH
