Streptomyces coelicolor genome carries two apparently paralogous genes, SCO4164 and SCO5854, that encode putative thiosulfate sulfurtransferases (rhodaneses). These genes (and their presumed translation products) are highly conserved and widely distributed across actinobacterial genomes. The SCO4164 knockout strain was unable to grow on minimal media with either sulfate or sulfite as the sole sulfur source. The SCO5854 mutant had no growth defects in the presence of various sulfur sources; however, it produced significantly less amounts of actinorhodin. Furthermore, we discuss possible links between basic interconversions of inorganic sulfur species and secondary metabolism in S. coelicolor.
- MeSH
- anthrachinony metabolismus MeSH
- antibakteriální látky metabolismus MeSH
- bakteriální proteiny genetika metabolismus MeSH
- kultivační média metabolismus MeSH
- sekundární metabolismus MeSH
- sírany metabolismus MeSH
- Streptomyces coelicolor enzymologie genetika růst a vývoj metabolismus MeSH
- thiosulfátsulfurtransferasa genetika metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
This paper describes a method for the determination of cyanide using in-capillary enzymatic reaction with rhodanese. Poorly absorbing cyanide is in rhodanese reaction transformed into highly absorbing thiocyanate that is further separated by capillary electrophoresis (CE) and determined spectrophotometrically at 200 nm. Cyanide is thus estimated indirectly from the result of thiocyanate quantification and moreover, it can be easily determined with sufficient sensitivity by means of CE apparatus equipped with common UV detector. The linear detection range for concentration versus peak area for the assay is from 15 to 500 microM (correlation coefficient 0.997) with a detection limit of 3 microM and a limit of quantitation 9 microM. The inter-day reproducibility of the peak area was below 3.2% and the inter-day reproducibility of the migration time below 0.1%. The method is relatively rapid, simple and can be easily automated. Moreover, only limited amount of the sample is required.
