Clone library of bacterial 16S rRNA gene was constructed to evaluate the bacterial diversity and community structure of uterus samples obtained from three postpartum healthy cows and three metritic cows on days 10 and 40. Sequences were assigned to five major groups (Bacteroidetes, Firmicutes, Fusobacteria, Proteobacteria, and Tenericutes) and to an uncultured group. On day 10, Bacteroidetes, Firmicutes, and Fusobacteria were the dominant group both in healthy and metritic cows. On day 40, the major sequences were affiliated with Bacteroidetes, Firmicutes, Tenericutes, and Proteobacteria. Tenericutes (Ureaplasma diversum) were revealed only from healthy cows, while Proteobacteria (Histophilus somni) were found only from metritic cows. Quantitative PCR revealed that metritic cows on day 10 showed higher value of total bacteria, Bacteroidetes, Peptostreptococcus, and Fusobacterium compared with healthy cows, while only a higher value of Fusobacterium spp. was observed from the metritic cows on day 40 compared with that from healthy cows (P < 0.05). Our data indicates that great difference in the uterine bacterial community in both phyla level and species level exists between healthy and metritic postpartum cows, and dynamic changes in bacterial community occur over time.
- MeSH
- Bacteria klasifikace genetika MeSH
- bakteriální nálož MeSH
- časové faktory MeSH
- DNA bakterií chemie genetika MeSH
- endometritida mikrobiologie veterinární MeSH
- fylogeneze MeSH
- molekulární sekvence - údaje MeSH
- nemoci skotu mikrobiologie MeSH
- poporodní období MeSH
- ribozomální DNA chemie genetika MeSH
- RNA ribozomální 16S genetika MeSH
- sekvenční analýza DNA MeSH
- shluková analýza MeSH
- skot MeSH
- společenstvo * MeSH
- uterus mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- gynekologická onemocnění diagnóza terapie MeSH
- kolposkopie MeSH
- uterus mikrobiologie patologie MeSH
- Publikační typ
- biografie MeSH
- O autorovi
- Mendl, Vladimír, 1927-1998 Autorita
- MeSH
- cervix uteri mikrobiologie MeSH
- Chlamydia trachomatis izolace a purifikace MeSH
- dospělí MeSH
- lidé MeSH
- Ureaplasma urealyticum izolace a purifikace MeSH
- uterus abnormality mikrobiologie MeSH
- vagina abnormality mikrobiologie MeSH
- ženské pohlavní orgány abnormality MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
The author tested ways of collection, transport and storage of material for mycoplasmatological examination. The use of tampons on a stick during transport in urea substrate medium proved useful. The site of maximum occurrence of mycoplasmas was the posterior vaginal vault. On examination of the vaginal secretion of 804 women Mycoplasma hominis was isolated in 29.6% and Ureaplasma urealyticum in 65.2% of the cases. Concurrent isolation of Mycoplasma hominis and Ureaplasma urealyticum was recorded in 22.1% of the women. The results of the examination do not suggest the participation of mycoplasmas in the development of aminocolpitis. In pregnant women there is a greater probability of colonization of the vagina by U. urealyticum.
- MeSH
- lidé MeSH
- Mycoplasma izolace a purifikace MeSH
- Ureaplasma izolace a purifikace MeSH
- uterus mikrobiologie MeSH
- vagina mikrobiologie MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
