- MeSH
- apoptóza MeSH
- buněčné jádro ultrastruktura MeSH
- karcinom patologie MeSH
- kolorektální nádory patologie ultrastruktura MeSH
- koncové značení zlomů DNA in situ metody MeSH
- lidé MeSH
- nehodgkinský lymfom patologie ultrastruktura MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- srovnávací studie MeSH
- Geografické názvy
- Česká republika MeSH
OBJECTIVE: A model of estrogen (E)-induced rat anterior pituitary hyperplasia blocked partly by thyroid hormones (estradiol plus thyreoidin [ET]) and nearly completely by lisuride (estradiol plus lisuride [EL]) and thyreoidin plus lisuride combination (estradiol plus thyreoidin plus lisuride [ETL]) was used to test a computer image analysis program, LUCIA, for argyrophilic nucleolar organizer region (AgNOR) evaluation. STUDY DESIGN: Nuclear and AgNOR area were measured in more than 700 cells in each experimental group. The nucleoli were also typed without the automated procedure. RESULTS: Mean nuclear area was significantly higher in E and lower in T. In all groups with blocked hyperplasia (ET, EL, ETL) the mean nuclear area was lower than in T. Mean AgNOR area was significantly higher in E but also, to a lesser extent, in T. The ET combination produced a partly diminished mean AgNOR area as compared to E; a severe reduction was observed in the EL and ETL groups. In nucleolar typing, thyreoidin, lisuride and their combination inhibited the increase in large nucleoli with finely dispersed AgNORs induced by estradiol. CONCLUSION: The LUCIA image analysis program for AgNOR evaluation proved able to monitor the slight changes in AgNORs in thyroid hormones- and lisuride-inhibited, estradiol-induced rat anterior pitutary hyperplasia.
- MeSH
- adenohypofýza patologie účinky léků MeSH
- antagonisté dopaminu MeSH
- estradiol farmakologie MeSH
- krysa rodu rattus MeSH
- lisurid farmakologie MeSH
- organizátor jadérka patologie MeSH
- počítačové zpracování obrazu metody MeSH
- senzitivita a specificita MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- zvířata MeSH
OBJECTIVE: To characterize differences in the depth of fluorescent probes, to observe estimated depth levels (focal planes) on fluorescent in situ hybridization preparations by factor analysis of medical image sequences and to use cytogenetic techniques resulting in flat preparations of whole cells that are assumed to preserve the probes' access to their targets in the human nuclear interphase. STUDY DESIGN: We used labeling of the targets by the probes (sequences labeled by fluoroscein isothiocyonate [FITC]) in the nuclei, stained by propidium iodide. The investigation was performed on this model by three-dimensional (3-D) sequences of images obtained on a single photomultiplier detector of a confocal microscope by selection of emission between 510 and 550 nm and by (z) displacement. The investigation was also performed by obtaining sequences of images from spherical fluorescent beads to verify (z) focusing, to visualize depth differences and to analyze estimated factor images. RESULTS: Estimated images showed depth differences in FITC-stained targets as well as in nuclei, stained with propidium iodide, in interphase and in fluorescent beads, that could not be visualized by conventional 3-D reconstruction. CONCLUSION: It is possible to study 3-D architecture of flattened preparations and penetration of fluorophores inside the beads and to evaluate depth differences.
- MeSH
- buněčné jádro ultrastruktura MeSH
- fluorescein-5-isothiokyanát chemie MeSH
- fluorescenční barviva chemie MeSH
- histocytologické preparační techniky MeSH
- hybridizace in situ fluorescenční * MeSH
- konfokální mikroskopie MeSH
- lidé MeSH
- lymfocyty cytologie MeSH
- počítačové zpracování obrazu MeSH
- propidium chemie MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
OBJECTIVE: To determine the three-dimensional (3D) position of target sequences and chromosomal volumes in interphase human nuclei by confocal laser scanning microscopy (CLSM) by using the heterochromatin part of the long arm of human chromosome Y (HPLAHC) as a target for specific DYZ1 probes, then by D10Z1 probes specific to the centromere of chromosome 10. STUDY DESIGN: Fluorescence in situ hybridization information inside chromosomal preparations was obtained with FITC-labelled probes and propidium iodide (PI) as a DNA-specific stain. To have a control in the experiment, HPLAHC Y was taken as a model of a domain and the centromere of chromosome 10 as a model of a single centromere spot. To have access to their 3D visualization, we selected FITC and PI patterns of fluorescence when optical slices were obtained and used a 3D reconstruction software. RESULTS: Labelling of the target by the probes was characteristic of Y heterochromatin and chromosome 10 centromere localizations and allowed observation of their domain in the x, y and z directions. CONCLUSION: This work was performed on two sets of 30 stained interphase nuclei. Deformations were confirmed by fluorescent spherical beads mounted in the same medium and scanned in the same conditions.
- MeSH
- buněčné jádro MeSH
- centromera MeSH
- chromozom Y * MeSH
- DNA sondy MeSH
- heterochromatin MeSH
- hybridizace in situ fluorescenční * metody MeSH
- interfáze MeSH
- konfokální mikroskopie MeSH
- kultivované buňky MeSH
- lidé MeSH
- lidské chromozomy, pár 10 * MeSH
- lymfocyty cytologie MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH