Cationic quaternized carbon dots (QCDs) and anionic graphene oxide sheets (GO) are combined via non-covalent interactions following a self-assembly pathway to form highly biocompatible and fluorescent hybrid materials. These hybrids act as selective probes with controlled labelling of the cell nucleus or cytoplasm depending on the QCD loading.

• MeSH
• buněčné jádro chemie   metabolismus   MeSH
• buňky NIH 3T3 MeSH
• cytoplazma chemie   metabolismus   MeSH
• grafit chemie   MeSH
• kationty chemie   MeSH
• konfokální mikroskopie MeSH
• kvantové tečky chemie   metabolismus   MeSH
• myši MeSH
• oxidy chemie   MeSH
• uhlík chemie   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Many photosensitive substances suitable for photodynamic therapy (PDT) have limited applications due to their insufficient solubility in polar solvents. Our research overcomes this challenge by means of nanotechnology in order to transform hydrophobic compounds into stable aqueous solutions, enabling them to use their full potential and unique properties in cancer therapy. In this study, the novel nano-composite cGQDs-PEG-curcumin was developed to overcome the insolubility of curcumin in water and its extraordinary efficacy in PDT was evaluated. Complex characterization was performed using high-resolution transmission electron microscopy (HR-TEM), FTIR, and UV-Vis spectroscopy. Further analysis involved fluorescence lifetime imaging (FLIM), and its cellular localization was mapped with confocal microscopy. In order to evaluate PDT effectiveness, cells treated with cGQDs-PEG-curcumin were irradiated with 5 J/cm2 of 414 nm light. After irradiation, cell viability assay, scanning electron microscopy (SEM), reactive oxygen species (ROS) detection, comet assay, and γH2AX-based DNA double-strand breaks (DSBs) detection were assessed and revealed a remarkable ability of the nano-composite to induce DNA damage after irradiation without ROS production. Our findings highlight the potential of cGQDs-PEG-curcumin as a cutting-edge PDT agent, capable of disrupting cell membrane and nucleolar integrity and impairing ribosomal synthesis, which is crucial for proliferating tumour cells.

• MeSH
• buněčné jadérko * účinky léků   metabolismus   MeSH
• dvouřetězcové zlomy DNA účinky léků   MeSH
• fotochemoterapie * metody   MeSH
• fotosenzibilizující látky * farmakologie   MeSH
• grafit * chemie   farmakologie   MeSH
• kurkumin * farmakologie   chemie   MeSH
• kvantové tečky * chemie   MeSH
• lidé MeSH
• nádorové buněčné linie MeSH
• nádory * farmakoterapie   MeSH
• polyethylenglykoly * chemie   farmakologie   MeSH
• poškození DNA * účinky léků   MeSH
• reaktivní formy kyslíku metabolismus   MeSH
• viabilita buněk účinky léků   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

The supply of safe drinking and clean water is becoming increasingly challenging proposition throughout the world. The deployment of environmentally sustainable nanomaterials with unique advantages namely high efficiency and selectivity, earth-abundance, recyclability, low-cost of production processes, and stability, has been a priority although several important challenges and constraints still remained unresolved. Carbon nanomaterials namely activated carbon, multi-walled- and single-walled carbon nanotubes, have been developed and applied as adsorbents for wastewater treatment and purification; graphene and graphene oxide-based nanomaterials as well as carbon and graphene quantum dots-derived nanomaterials have shown significant promise for water and wastewater treatment and purification, especially, for industrial- and pharmaceutical-laden wastes. This review encompasses advanced carbonaceous nanomaterials and methodologies that are deployed for the elimination of contaminants and ionic metals in aqueous media, and as novel nanosorbents for wastewater, drinking and ground water treatment. Additionally, recent trends and challenges pertaining to the sustainable carbon and graphene quantum dots-derived nanomaterials and their appliances for treating and purifying wastewater are highlighted.

• MeSH
• čištění vody * MeSH
• nanostruktury * MeSH
• nanotrubičky uhlíkové * MeSH
• odpadní voda MeSH
• voda MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH

We review the progress achieved during the recent five years in immunochemical biosensors (immunosensors) combined with nanoparticles for enhanced sensitivity. The initial part introduces antibodies as classic recognition elements. The optical sensing part describes fluorescent, luminescent, and surface plasmon resonance systems. Amperometry, voltammetry, and impedance spectroscopy represent electrochemical transducer methods; electrochemiluminescence with photoelectric conversion constitutes a widely utilized combined method. The transducing options function together with suitable nanoparticles: metallic and metal oxides, including magnetic ones, carbon-based nanotubes, graphene variants, luminescent carbon dots, nanocrystals as quantum dots, and photon up-converting particles. These sources merged together provide extreme variability of existing nanoimmunosensing options. Finally, applications in clinical analysis (markers, tumor cells, and pharmaceuticals) and in the detection of pathogenic microorganisms, toxic agents, and pesticides in the environmental field and food products are summarized.

• MeSH
• biosenzitivní techniky * MeSH
• imunoanalýza * MeSH
• lidé MeSH
• nanočástice chemie   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH

Trifluralin is herbicide of the dinitroanilines group in which NO2 molecules are attached to the benzene ring at diverse positions. Trifluralin affects endocrine function and is listed as an endocrine disrupter in the European Union list. Therefore, its determination is so important in health science. In this study, an easy, sensitive and environmentally friendly method has been developed for determination of trifluralin based on its electrochemical oxidation on a three-electrode system designed on the surface of agricultural products using Ag-citrate/GQDs (graphene quantum dots) nano-ink. The sensor was prepared by direct writing on the surface of the samples. The designed electrodes were dried after 24 h at room temperature and used for trifluralin detection. Under optimized experimental conditions, the Ag-citrate/GQDs nano-ink based sensor was exhibited good sensitivity and specificity for trifluralin detection. The obtained linear range using the cyclic voltammetric (CV) technique is between 0.008 to 1 mM and low limit of quantification (LLOQ) was 0.008 mM. Also, the obtained linear range using differential pulse voltammetric (DPV) and square wave voltammetric (SWV) techniques is 0.005-0.04 mM with LLOQ of 0.005 mM. For further validation of the applicability of the proposed method, it was also used for detection of trifluralin on the surface of apple skin.

• Publikační typ
• časopisecké články MeSH