Porcine model of peritonitis-induced sepsis is a well-established clinically relevant model of human disease. Interindividual variability of the response often complicates the interpretation of findings. To better understand the biological basis of the disease variability, the progression of the disease was compared between animals with sepsis and septic shock. Peritonitis was induced by inoculation of autologous feces in fifteen anesthetized, mechanically ventilated and surgically instrumented pigs and continued for 24 h. Cardiovascular and biochemical parameters were collected at baseline (just before peritonitis induction), 12 h, 18 h and 24 h (end of the experiment) after induction of peritonitis. Analysis of multiple parameters revealed the earliest significant differences between sepsis and septic shock groups in the sequential organ failure assessment (SOFA) score, systemic vascular resistance, partial pressure of oxygen in mixed venous blood and body temperature. Other significant functional differences developed later in the course of the disease. The data indicate that SOFA score, hemodynamical parameters and body temperature discriminate early between sepsis and septic shock in a clinically relevant porcine model. Early pronounced alterations of these parameters may herald a progression of the disease toward irreversible septic shock.

• Publikační typ
• časopisecké články MeSH

Bacterial resistance increasingly limits antibiotic effectiveness, and concurrently significantly complicates antibiotic therapy of systemic infections, e.g. sepsis, resulting in higher morbidity and mortality of patients. A promising way to overcome this problem is a combination of classic antibiotics with silver nanoparticles (NPs). Thanks to non-specific activity of silver NPs, synergistic combination enables strong and effective antibacterial activity at low doses both of antibiotics and silver NPs and at the same time limits development of bacterial resistance. The aim of this project is in vitro and in vivo applied research focused on possible use of conventional antibiotics potentiated with silver NPs in therapy of systemic infection in clinically relevant large animal model of sepsis.

Bakteriální rezistence stále více limituje účinnost antibiotik a rovněž velmi výrazně komplikuje schopnost léčby závažných systémových infekcí, například sepsí, což způsobuje nárůst morbidity a mortality pacientů. Slibnou možností jak překonat tento problém je kombinace klasických antibiotik s nanočásticemi stříbra. Takováto synergická kombinace umožňuje díky nespecifické aktivitě nanostříbra vysoce efektivní antibakteriální účinnost za nízkých koncentrací jak antibiotika, tak i nanočástic stříbra a současně limituje vývoj bakteriální rezistence. Cílem tohoto projektu je in vitro a in vivo aplikovaný výzkum zaměřený na možnosti použití nanočásticemi stříbra potencovaných antibiotik v léčbě systémové infekce u klinicky relevantního modelu sepse na velkém zvířeti.

• Klíčová slova
• rezistence, resistance, sepse, sepsis, infekce, infection, bakterie, bacteria, Nanočástice, in vivo, in vitro, in vivo, in vitro, nanoparticle, Antibiotikum, Antibiotic, silver, antibacterial activity, synergy, stříbro, antibakteriální aktivita, synergie,

• NLK Publikační typ
• závěrečné zprávy o řešení grantu AZV MZ ČR