We present a biological profile of 16 Aspergillus niger environmental isolates from different types of soils and solid substrates across a pH range, from an ultra-acidic (<3.5) to a very strongly alkaline (>9.0) environment. The soils and solid substrates also differ in varying degrees of anthropic pollution, which in most cases is caused by several centuries of mining activity at old mining sites, sludge beds, ore deposits, stream sediments, and coal dust. The values of toxic elements (As, Sb, Zn, Cu, Pb) very often exceed the limit values. The isolates possess different macro- and micromorphological features. All the identifications of Aspergillus niger isolates were confirmed by molecular PCR analysis and their similarity was expressed by RAMP analysis. The biochemical profile of isolates based on FF-MicroPlate tests from the Biolog system showed identical biochemical reactions in 50 tests, while in 46 tests the utilisation reactions differed. The highest similarity of strains isolated from substrates with the same pH, as well as the most suitable biochemical tests for analysis of the phenotypic similarity of isolated strains, were confirmed when evaluating the biochemical profile using multicriterial analysis in the Canoco program. The isolates were screened for mycotoxin production by thin-layer chromatography (TLC), as well. Two of them were able to synthesise ochratoxin A, while none produced fumonisins under experimental conditions. Presence of toxic compounds in contaminated sites may affect environmental microscopic fungi and cause the genome alteration, which may result in changes of their physiology, including the production of different (secondary) metabolites, such as mycotoxins.

• Publikační typ
• časopisecké články MeSH

Obsáhlý soubor 70 kazuistik dětských pacientů s monogenními onemocněními, na němž se podílel více než stočlenný autorský kolektiv odborníků z České republiky i ze zahraničí, obsahuje ty nejzajímavější případy z jejich klinické praxe. Autoři se soustředili jak na nejčastější choroby, tak i na onemocnění vzácná, ale z různých pohledů důležitá.

• MeSH
• genetická predispozice k nemoci MeSH
• genetická zátěž MeSH
• genetické nemoci vázané na chromozom X MeSH
• genetické nemoci vrozené MeSH
• pediatrie MeSH
• Publikační typ
• kazuistiky MeSH
• sborníky MeSH

• Konspekt
• Patologie. Klinická medicína
• NLK Obory
• genetika, lékařská genetika
• biologie
• pediatrie