This is the first report describing precipitation of bacteriophage particles with zinc chloride as a method of choice to isolate infectious lytic bacteriophages against Pectobacterium spp. and Dickeya spp. from environmental samples. The isolated bacteriophages are ready to use to study various (ecological) aspects of bacteria-bacteriophage interactions. The method comprises the well-known precipitation of phages from aqueous extracts of the test material by addition of ZnCl2, resuscitation of bacteriophage particles in Ringer's buffer to remove the ZnCl2 excess and a soft agar overlay assay with the host bacterium to isolate infectious individual phage plaques. The method requires neither an enrichment step nor other steps (e. g., PEG precipitation, ultrafiltration, or ultracentrifugation) commonly used in other procedures and results in isolation of active viable bacteriophage particles.

• MeSH
• bakteriofágy izolace a purifikace   fyziologie   MeSH
• časové faktory MeSH
• chloridy metabolismus   MeSH
• Enterobacteriaceae virologie   MeSH
• mikrobiologie životního prostředí * MeSH
• Pectobacterium virologie   MeSH
• rostliny virologie   MeSH
• sloučeniny zinku metabolismus   MeSH
• virologie metody   MeSH
• Publikační typ
• časopisecké články MeSH