Two novel dsDNA bacteriophages named Pectobacterium virus CB251 (PcCB251) and Pectobacterium virus CB7V (PcCB7V) targeting plant pathogen Pectobacterium parmentieri have been isolated and sequenced. The PcCB251 genome consists of 40,557 bp with G+C content of 48.6% and contains 47 predicted genes on a single strand. The phage is classified in genus Berlinvirus, family Autographiviridae. The PcCB7V phage has a circular dsDNA genome of 146,054 bp with G+C content of 50.4% and contains 269 predicted protein genes on both strands and 13 tRNA genes. The PcCB7V phage can be classified in genus Certrevirus, subfamily Vequintavirinae. Both novel bacteriophages have narrow host ranges, but they extend the list of candidates for phage-based control of pectolytic bacteria causing soft rot disease of potato.

• MeSH
• bakteriofágy genetika   MeSH
• DNA viry genetika   MeSH
• genom virový genetika   MeSH
• Pectobacterium genetika   patogenita   virologie   MeSH
• rostlinné viry genetika   patogenita   MeSH
• sekvenování celého genomu MeSH
• Solanum tuberosum genetika   virologie   MeSH
• Publikační typ
• časopisecké články MeSH

This is the first report describing precipitation of bacteriophage particles with zinc chloride as a method of choice to isolate infectious lytic bacteriophages against Pectobacterium spp. and Dickeya spp. from environmental samples. The isolated bacteriophages are ready to use to study various (ecological) aspects of bacteria-bacteriophage interactions. The method comprises the well-known precipitation of phages from aqueous extracts of the test material by addition of ZnCl2, resuscitation of bacteriophage particles in Ringer's buffer to remove the ZnCl2 excess and a soft agar overlay assay with the host bacterium to isolate infectious individual phage plaques. The method requires neither an enrichment step nor other steps (e. g., PEG precipitation, ultrafiltration, or ultracentrifugation) commonly used in other procedures and results in isolation of active viable bacteriophage particles.

• MeSH
• bakteriofágy izolace a purifikace   fyziologie   MeSH
• časové faktory MeSH
• chloridy metabolismus   MeSH
• Enterobacteriaceae virologie   MeSH
• mikrobiologie životního prostředí * MeSH
• Pectobacterium virologie   MeSH
• rostliny virologie   MeSH
• sloučeniny zinku metabolismus   MeSH
• virologie metody   MeSH
• Publikační typ
• časopisecké články MeSH