• MeSH
• celulosa analogy a deriváty   chemie   MeSH
• gelová chromatografie metody   MeSH
• molekulová hmotnost MeSH
• refraktometrie MeSH
• světlo MeSH

Plausible calf-thymus DNA molecular weight distribution can be obtained by size-exclusion chromatography with dual low-angle light scattering/refractometric detection at sufficiently low flow rate. The distribution extends over three decades of molecular weight and is characterized by weight average molecular weight M(w) = 8418000 and polydispersity index M(w)/M(n) = 5.2. After strongly scattering impurities had been removed from the sample using adsorption properties of the 3 mum mixed-cellulose-ester filter membranes, static light-scattering measurement in flow injection mode was feasible and gave M(w) = 8580000, corroborating the veracity of SEC results.

• MeSH
• DNA analýza   chemie   MeSH
• gelová chromatografie metody   MeSH
• molekulová hmotnost MeSH
• radiační rozptyl MeSH
• skot MeSH
• světlo MeSH
• zvířata MeSH
• Check Tag
• skot MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH

We have developed a biodegradable, biocompatible system for the delivery of the antituberculotic antibiotic rifampicin with a built-in drug release and nanoparticle degradation fluorescence sensor. Polymer nanoparticles based on poly(ethylene oxide) monomethyl ether-block-poly(ε-caprolactone) were noncovalently loaded with rifampicin, a combination that, to best of our knowledge, was not previously described in the literature, which showed significant benefits. The nanoparticles contain a Förster resonance energy transfer (FRET) system that allows real-time assessment of drug release not only in vitro, but also in living macrophages where the mycobacteria typically reside as hard-to-kill intracellular parasites. The fluorophore also enables in situ monitoring of the enzymatic nanoparticle degradation in the macrophages. We show that the nanoparticles are efficiently taken up by macrophages, where they are very quickly associated with the lysosomal compartment. After drug release, the nanoparticles in the cmacrophages are enzymatically degraded, with half-life 88±11 min.

• MeSH
• antituberkulotika aplikace a dávkování   MeSH
• biokompatibilní materiály chemie   MeSH
• makrofágy účinky léků   metabolismus   MeSH
• myši MeSH
• nanočástice chemie   MeSH
• polyestery chemie   MeSH
• polyethylenglykoly chemie   MeSH
• RAW 264.7 buňky MeSH
• rezonanční přenos fluorescenční energie MeSH
• rifampin aplikace a dávkování   MeSH
• systémy cílené aplikace léků * MeSH
• uvolňování léčiv * MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH