This article describes the characterization and application of collagenase-based chitosan nanofiber membranes with rat burns. Electrospun chitosan nanofibers were functionalized with clostridial collagenase using carbodiimide chemistry. The immobilized collagenase was characterized by enzyme activity, kinetic constants, and dry storage stability measurements using a Pz-peptide substrate. The apparent kinetic constants KM and Vmax of immobilized collagenase showed a high affinity for the peptide substrate compared to the free enzyme. Drying of chitosan membranes with immobilized collagenase ensured 98 % stability of enzyme activity after rehydration. The effect of collagenase immobilized on chitosan nanofibers on the burn of the rat model was compared with a control treatment with chitosan nanofibers. The healing of the wound with both materials was terminated after 30 days at the same time, although the collagenase wound healed more rapidly during healing. The scar area size after the application of collagenase-containing chitosan nanofiber membranes was 31.6 % smaller than when only chitosan nanofibers were used.

• MeSH
• chitosan terapeutické užití   MeSH
• Clostridium histolyticum MeSH
• enzymy imobilizované MeSH
• hojení ran * účinky léků   MeSH
• krysa rodu rattus MeSH
• kůže zranění   MeSH
• mikrobiální kolagenasa * metabolismus   terapeutické užití   MeSH
• nanovlákna terapeutické užití   MeSH
• pilotní projekty MeSH
• rány a poranění farmakoterapie   patologie   MeSH
• stabilita enzymů MeSH
• výsledek terapie MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• zvířata MeSH