hydrostatic load Dotaz Zobrazit nápovědu
Meniscus is a semilunar fibrocartilaginous tissue, serving important roles in load buffering, stability, lubrication, proprioception, and nutrition of the knee joint. The degeneration and damage of meniscus has been proved to be a risk factor of knee osteoarthritis. Mechanical stimulus is a critical factor of the development, maintenance and repair of the meniscus fibrochondrocytes. However, the mechanism of the mechano-transduction process remains elusive. Here we reported that cyclic hydrostatic compress force (CHCF) treatment promotes proliferation and inhibits apoptosis of the isolated primary meniscus fibrochondrocytes (PMFs), via upregulating the expression level of integrin ?5ß1. Consequently, increased phosphorylated-ERK1/2 and phosphorylated-PI3K, and decreased caspase-3 were detected. These effects of CHCF treatment can be abolished by integrin ?5ß1 inhibitor or specific siRNA transfection. These data indicate that CHCF regulates apoptosis of PMFs via integrin ?5ß1-FAK-PI3K/ERK pathway, which may be an important candidate approach during meniscus degeneration.
- MeSH
- apoptóza fyziologie účinky léků MeSH
- buněčný převod mechanických signálů fyziologie MeSH
- chondrocyty metabolismus účinky léků MeSH
- fibroblasty metabolismus účinky léků MeSH
- hydrostatický tlak MeSH
- integrin alfa5beta1 antagonisté a inhibitory metabolismus MeSH
- krysa rodu rattus MeSH
- kultivované buňky MeSH
- malá interferující RNA aplikace a dávkování MeSH
- meniskus cytologie metabolismus MeSH
- pevnost v tlaku fyziologie MeSH
- potkani Sprague-Dawley MeSH
- proliferace buněk fyziologie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
The main goal of this study is to develop a micromechanical model of a particle-filled dental composite focused on the residual stress (RS) field developed during the curing process in its microstructure. A finite element model of a representative volume element of filler and resin was developed, and volumetric shrinkage was simulated during the curing process. Four material models (von Mises plasticity model, Drucker-Prager plasticity model, von Mises plasticity model with stress relaxation and Drucker-Prager plasticity with stress relaxation) of the polymer resin were built to assess the influence of the material model on the resulting internal stress. The relationship between the curing process and the magnitude of the stress components will be described, and an analysis of the post-curing state of the material in particular microstructure locations will be conducted in this study. Obtained RS is comparable to the stresses developed in the material under the external load. The substantial dependence on the choice of material model for resin is to be observed, and the suitability of particular models is discussed.
B.i Pseudonremic and pseudorachitics syndromes -- evoked by enduance load and nutrition 229 -- 4BartftAková S. - Havlíčková L. - Jetek P. - Paŕizkovi J.: Drinking -- regime daring an extreme endurance load . 2 -- JUrlmäe T. - Jagomägl C.: The new apparatuses for measurement -- body density by hydrostatic weighing
305 s. : obr., tab., bibliogr.
Adoptive T cell transfer has been shown to be an effective method used to boost tumor-specific immune responses in several types of malignancies. In this study, we set out to optimize the ACT protocol for the experimental treatment of prostate cancer. The protocol includes a pre-stimulation step whereby T cells were primed with autologous dendritic cells loaded with the high hydrostatic pressure-treated prostate cancer cell line, LNCaP. Primed T cells were further expanded in vitro with anti-CD3/CD28 Dynabeads in the WAVE bioreactor 2/10 system and tested for cytotoxicity. Our data indicates that the combination of pre-stimulation and expansion steps resulted in the induction and enrichment of tumor-responsive CD4(+) and CD8(+) T cells at clinically relevant numbers. The majority of both CD4(+) and CD8(+) IFN-γ producing cells were CD62L, CCR7 and CD57 negative but CD28 and CD27 positive, indicating an early antigen experienced phenotype in non-terminal differentiation phase. Expanded T cells showed significantly greater cytotoxicity against LNCaP cells compared to the control SKOV-3, an ovarian cancer line. In summary, our results suggest that the ACT approach together with LNCaP-loaded dendritic cells provides a viable way to generate prostate cancer reactive T cell effectors that are capable of mounting efficient and targeted antitumor responses and can be thus considered for further testing in a clinical setting.
- MeSH
- aktivace lymfocytů MeSH
- antigeny nádorové imunologie MeSH
- bioreaktory MeSH
- dendritické buňky imunologie MeSH
- epitopy T-lymfocytární imunologie MeSH
- imunoterapie adoptivní metody MeSH
- interferon gama biosyntéza imunologie MeSH
- lidé MeSH
- nádorové buněčné linie MeSH
- nádory prostaty imunologie MeSH
- nádory vaječníků imunologie MeSH
- regulační T-lymfocyty imunologie MeSH
- studie případů a kontrol MeSH
- T-lymfocyty - podskupiny imunologie MeSH
- T-lymfocyty imunologie MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
