Gestational diabetes mellitus (GDM) is a common complication of pregnancy in which women without previously diagnosed diabetes develop chronic hyperglycemia during pregnancy. It is associated with a number of maternal and fetal/neonatal complications. The role of the adipokines retinol binding protein-4, resistin and nesfatin-1 in the development of GDM is relatively poorly understood, but their role in glucose metabolism is suspected and their use as early markers to predict the development of GDM is being sought. The aim of study was to determine the correlation between the levels of selected adipokines (retinol binding protein-4, resistin, nesfatin-1) in women with gestational diabetes mellitus (GDM) and healthy pregnant women and to compare their levels with other clinical and biochemical parameters. Patients with GDM had significantly higher BMI (28.4±4.5 vs. 24.6±4 kg/m2), total cholesterol (6±1.3 vs. 5.3±1.4 mmol/l) and triacylglycerols (1.9±0.8 vs. 1.4±0.7 mmol/l) than women in the control group. RBP4 confirms the significant difference between the groups, it is higher in the control group of healthy pregnant women. The adipokines resistin and nesfatin-1 show no differences between the control and GDM groups, but their ratios with BMI, cholesterol and triacylglycerols, resistin shows elevated levels in the control group. In women with GDM, RBP4 was significantly positively correlated with C-peptide and negatively correlated with total, LDL, and non-HDL cholesterol. Resistin was also negatively correlated with total, LDL, HDL, and non-HDL cholesterol. Nesfatin-1 was only moderately positively correlated with glycated hemoglobin (HbA1C) and fasting glycemia. There is ambiguity in the results of previous studies on the levels of the investigated adipokines in pregnant women with GDM and the interpretation depends on many factors. Keywords: Gestational diabetes, Adipokines, Retinol-binding protein 4, Resistin, Nesfatin-1.
- MeSH
- adipokiny krev MeSH
- biologické markery * krev MeSH
- DNA vazebné proteiny krev MeSH
- dospělí MeSH
- gestační diabetes * krev diagnóza MeSH
- krevní glukóza metabolismus MeSH
- lidé MeSH
- nukleobindiny * krev MeSH
- plazmatické proteiny vázající retinol * metabolismus analýza MeSH
- proteiny nervové tkáně krev MeSH
- proteiny vázající vápník krev MeSH
- resistin * krev MeSH
- studie případů a kontrol MeSH
- těhotenství MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
BACKGROUND: Adipose tissue is significantly involved in inflammatory bowel disease (IBD). Vitamin D can affect both adipogenesis and inflammation. The aim of this study was to compare the production of selected adipokines, potentially involved in the pathogenesis of IBD - adiponectin, resistin, retinol binding protein 4 (RBP-4), adipocyte fatty acid binding protein and nesfatin-1 in children with IBD according to the presence of 25-hydroxyvitamin D (25(OH)D) deficiency. METHODS: The study was conducted as a case-control study in pediatric patients with IBD and healthy children of the same sex and age. In addition to adipokines and 25(OH)D, anthropometric parameters, markers of inflammation and disease activity were assessed in all participants. RESULTS: Children with IBD had significantly higher resistin levels regardless of 25(OH)D levels. IBD patients with 25(OH)D deficiency only had significantly lower RBP-4 compared to healthy controls and also compared to IBD patients without 25(OH)D deficiency. No other significant differences in adipokines were found in children with IBD with or without 25(OH)D deficiency. 25(OH)D levels in IBD patients corelated with RBP-4 only, and did not correlate with other adipokines. CONCLUSIONS: Whether the lower RBP-4 levels in the 25(OH)D-deficient group of IBD patients directly reflect vitamin D deficiency remains uncertain. The production of other adipokines does not appear to be directly related to vitamin D deficiency.
- MeSH
- adipokiny * krev MeSH
- adiponektin krev nedostatek MeSH
- biologické markery krev MeSH
- dítě MeSH
- DNA vazebné proteiny krev MeSH
- idiopatické střevní záněty krev komplikace MeSH
- lidé MeSH
- mladiství MeSH
- nedostatek vitaminu D * komplikace krev MeSH
- nukleobindiny krev MeSH
- plazmatické proteiny vázající retinol metabolismus analýza MeSH
- proteiny vázající mastné kyseliny krev MeSH
- proteiny vázající vápník krev MeSH
- resistin krev MeSH
- studie případů a kontrol MeSH
- vitamin D * krev analogy a deriváty MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Rheumatoid arthritis (RA) is a chronic multisystem illness that affects millions of individuals. The primary goal of RA therapy is to improve patients' quality of life by reducing pain severity, preserving or improving functional capability, and decreasing disability. The significance of resistin in the pathophysiology of RA has been explored in recent years, although its role is unclear as it is largely produced by macrophages. In this review, we have analyzed 10 studies from the PubMed site that demonstrate a relationship between resistin levels and the severity of RA.
- MeSH
- cytokiny škodlivé účinky MeSH
- klinická studie jako téma MeSH
- lidé MeSH
- resistin * škodlivé účinky MeSH
- revmatoidní artritida * farmakoterapie patofyziologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- přehledy MeSH
Bariatric procedures are considered to be the most effective treatment options for obesity. One of them is laparoscopic sleeve gastrectomy (LSG), which is nowadays very popular and widely used. LSG leads to weight loss and metabolic improvement and also changes adipokine levels, although it is just a restrictive operation. We describe changes in pro-inflammatory (leptin, resistin, visfatin and chemerin) and anti-inflammatory adipokines (adiponectin, omentin), with adiponectin and leptin being most studied. Their levels are markedly changed after LSG and this may partially explain the weight loss seen after LSG. Adipokines are closely connected to insulin resistance and chronic inflammation both being positively influenced after LSG. Leptin regulates amount of body fat, appetite, thermogenesis and metabolic rate and its levels are positively correlated with both weight and BMI changes after operation. Resistin influences insulin sensitivity, modulates body cholesterol trafficking and its changes after operation correlate with BMI, waist circumference, fat mass, LDL cholesterol and C-reactive protein. Chemerin, an important component of immune system, decreases after bariatric surgery and its levels correlate with BMI, triglyceride levels, and blood glucose. On the other hand, pro-inflammatory adipokine adiponectin, which influences fatty acid oxidation, browning of fat tissue and energy metabolism, is declining after LSG. This decline explains improvement of glucose status after bariatric surgery in patients with diabetes and is correlated with BMI loss, waist circumference and LDL cholesterol level. Effect of LSG goes beyond calory restriction and the changes of adipokines have a great impact on health status of the bariatric patients.
- MeSH
- adipokiny MeSH
- adiponektin MeSH
- gastrektomie metody MeSH
- hmotnostní úbytek fyziologie MeSH
- inzulinová rezistence * fyziologie MeSH
- laparoskopie * metody MeSH
- LDL-cholesterol MeSH
- leptin MeSH
- lidé MeSH
- morbidní obezita * metabolismus chirurgie MeSH
- resistin MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Human adipocytes release multiple adipokines into the bloodstream during physical activity. This affects many organs and might contribute to the induction of inflammation. In this study, we aimed to assess changes in circulating adipokine levels induced by intense aerobic and anaerobic exercise in individuals with different adipose tissue content. In the quasi-experimental study, 48 male volunteers (aged 21.78 ± 1.98 years) were assigned to groups depending on their body fat content (BF): LBF, low body fat (<8% BF, n = 16); MBF, moderate body fat (8-14% BF, n = 19); and HBF, high body fat (>14% BF, n = 13). The volunteers performed maximal aerobic effort (MAE) and maximal anaerobic effort (MAnE) exercises. Blood samples were collected at five timepoints: before exercise, immediately after, 2 h, 6 h, and 24 h after each exercise. The selected cytokines were analyzed: adiponectin, follistatin-like 1, interleukin 6, leptin, oncostatin M, and resistin. While the participants' MAnE and MAE performance were similar regardless of BF, the cytokine response of the HBF group was different from that of the others. Six hours after exercise, leptin levels in the HBF group increased by 35%. Further, immediately after MAnE, resistin levels in the HBF group also increased, by approximately 55%. The effect of different BF was not apparent for other cytokines. We conclude that the adipokine exercise response is associated with the amount of adipose tissue and is related to exercise type.
- MeSH
- adipokiny * krev MeSH
- adiponektin MeSH
- cvičení fyziologie MeSH
- cytokiny MeSH
- leptin MeSH
- lidé MeSH
- resistin MeSH
- tuková tkáň * fyziologie MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Wnt1 inducible protein-1 signaling pathway (WISP-1) is a relatively new adipokine involved in many cellular processes, including epithelial mucosa healing. The aim of the study was to compare circulating levels of WISP-1 and other selected adipokines [adiponectin, resistin and retinol-binding protein 4 (RBP-4)] in children with inflammatory bowel disease (IBD) with healthy controls and to investigate possible differences between Crohn's disease patients. (CD) or ulcerative colitis (UC). The study was performed as a case-control study. In addition to adipokines, anthropometric, lipid parameters, markers of inflammation or disease activity were evaluated in all participants. Compared to healthy controls (n=20), significantly lower levels of adiponectin and higher levels of resistin and WISP-1 were found in patients with IBD (n=58). Elevation of WISP-1 was detected only in the CD group (n=31). There were no differences in RBP-4 levels between the groups. Adiponectin, WISP-1 and RBP-4 were independently associated with body mass index only, resistin levels were associated with C-reactive protein levels and leukocyte counts. Adverse adipokines production reflects presence of dysfunctional fat tissue in IBD patients. Higher levels of WISP-1 in CD compared to patients with UC may indicate a specific role for mesenteric adipose tissue in WISP-1 production.
- MeSH
- adipokiny MeSH
- adiponektin MeSH
- dítě MeSH
- idiopatické střevní záněty * MeSH
- lidé MeSH
- mezibuněčné signální proteiny CCN krev MeSH
- protoonkogenní proteiny krev MeSH
- resistin MeSH
- signální transdukce MeSH
- studie případů a kontrol MeSH
- ulcerózní kolitida * MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Higher serum resistin levels were reported to be associated with increased mortality risk. We aimed to assess the predictive value of resistin levels in perivascular adipose tissue (PVAT) around the left main coronary artery (LMCA) for mid-term survival of patients with advanced coronary artery disease (CAD).This was a prospective study including patients referred for elective coronary artery grafting in 2016 and 2017, performed using a standard approach. A sample of PVAT was harvested and resistin levels were measured using an enzyme-linked immunosorbent assay. Patients were followed from the day of the procedure until March 2021. In each patient, the SYNTAX score and EuroSCORE II were calculated. The study included 108 patients aged 68.1 ±7.9 years, including 83 men (76.9%). The duration of follow-up was 731 (range, 275-1020) for nonsurvivors and 1418 median (range, 1174-1559) for survivors (p <0.001). Patients who died had a higher SYNTAX score, higher EuroSCORE II, and lower resistin levels in PVAT than survivors (p <0.001, p = 0.004, and p = 0.041, respectively). A stepwise regression analysis revealed that survival was related to resistin concentrations above the median value (hazard ratio [HR], 4.67; 95% CI, 1.02-21.4; p = 0.048) and EuroSCORE II (used as continuous variable; HR, 1.55; 95% CI, 1.16-2.07; p = 0.003). The mid-term mortality in patients with advanced CAD is associated with low resistin concentrations in PVAT surrounding the LMCA.
- MeSH
- biologické markery metabolismus MeSH
- časové faktory MeSH
- ELISA MeSH
- hodnocení rizik MeSH
- koronární bypass * škodlivé účinky mortalita MeSH
- lidé středního věku MeSH
- lidé MeSH
- nemoci koronárních tepen diagnostické zobrazování metabolismus mortalita chirurgie MeSH
- prediktivní hodnota testů MeSH
- prospektivní studie MeSH
- resistin metabolismus MeSH
- rizikové faktory MeSH
- senioři MeSH
- tuková tkáň metabolismus MeSH
- výsledek terapie MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
This study aimed at evaluating the role played by insulin resistance, lipid metabolism disorder, oxidative stress, resistin, vaspin, Interleukin-18 and asymmetric dimethyl arginine as a marker for endothelial dysfunction in the pathogenesis of preeclampsia. This prospective observational cohort study involved 60 women who were classified into: 20 non-pregnant women (group 1 or control group), 20 normally pregnant women (group 2) and 20 preeclamptic women (group 3) at their third trimester. The pregnant women were assessed at their third trimester and further re-evaluated four weeks after delivery. The assessment included demography, assessment of proteinuria and urinary protein to creatinine ratio, blood pressure measurement and assessment of fasting blood glucose, fasting insulin level, lipid panel and the circulating levels of malondialdehyde, resistin, vaspin, interleukin-18 and asymmetric dimethyl arginine. Preeclamptic women showed more atherogenic lipid profile, significantly higher Homeostatic Model Assessment for Insulin Resistance (HOMA-IR) and significantly elevated levels of malondialdehyde, resistin, vaspin and interleukin-18 than the other study groups. Serum asymmetric dimethyl arginine concentration showed non-significant difference among the three study groups. The levels of resistin and vaspin showed significant decrease four weeks postpartum in preeclamptic group. We concluded that, preeclampsia was associated with insulin resistance, dyslipidemia, oxidative stress, inflammation and significant changes in adipokines; resistin and vaspin. Furthermore, the significant increase in the serum levels of resistin and vaspin at the third trimester and their significant decline four weeks postpartum in preeclamptic group focus the attention on the role played by these adipokines in the pathogenesis of preeclampsia.
BACKGROUND: It is commonly assumed that increased dietary fat and/or caloric excess induces chronic inflammatory processes, since the association between obesity and chronic adipose tissue with systemic inflammation has been shown previously. As far as we know, the reported health benefits of a VLCHF or ketogenic diet have not adequately involved an evaluation of biomarkers of inflammation. AIM: This study investigated the effects of a four-week very low-carbohydrate high-fat (VLCHF) diet in healthy young individuals on biomarkers of inflammation. METHODS: Eighteen moderately trained males (age 23.8 ± 2.1 years) were assigned to two groups. One group switched to a non-standardised VLCHF diet for four weeks, while the second group remained consuming their normal habitual diet (HD). Biomarkers of inflammation (adiponectin, leptin, resistin and interleukin-6) and substrate metabolism (fasting glucose and triacylglyceride concentrations) were analysed from blood at baseline and after four weeks. RESULTS: There was moderate evidence for substantial changes in leptin serum concentrations in the VLCHF group, with small to large decreases compared to the HD group after four weeks (effect size = 0.78, 95% CI 0.42, 0.93, p = 0.008; Bayes Factor10 = 5.70). No substantial between-group change differences over time were found across any other biomarkers. CONCLUSIONS: A four-week period of consuming a VLCHF diet in healthy young men was not associated with any considerable changes in markers of inflammation but showed evidence for lowered serum leptin concentrations relative to the HD group.
- MeSH
- adiponektin krev MeSH
- biologické markery krev MeSH
- dieta s omezením sacharidů metody MeSH
- dieta s vysokým obsahem tuků metody MeSH
- dietní tuky MeSH
- dospělí MeSH
- interleukin-6 krev MeSH
- ketogenní dieta metody MeSH
- krevní glukóza analýza MeSH
- leptin krev MeSH
- lidé MeSH
- mladý dospělý MeSH
- resistin krev MeSH
- triglyceridy krev MeSH
- zánět krev dietoterapie MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Úvod. Idiopatické zánětlivé myopatie (myozitidy, IIM), představují heterogenní skupinu vzácných autoimunitních systémových onemocnění, společně charakterizovaných zejména svalovou slabostí, postihující převážně proximální skupiny příčně pruhovaného svalstva. Heterogenita myozitid je více než v klinickém obraze vyjádřena v jejich patogenetických mechanismech a odráží se v imunofenotypové odpovědi u jednotlivých podtypů. Cíle práce. Cílem této práce bylo popsat asociace a vliv solubilních faktorů imunitního systému, přítomných v sérech pacientů s IIM, s fenotypovými charakteristikami myozitid a jejich podtypů, ověřit případnou expresi těchto molekul v zánětlivě změněné svalové tkáni pacientů a zhodnotit jejich význam v patogenezi analýzou jejich působení na imunitní a svalové buňky in vitro. Výsledky. Popsali jsme prevalenci a charakteristiku kloubního postižení u pacientů s myozitidou a jeho významnou asociaci s anti-Jo-1 autoprotilátkou. Dále jsme potvrdili vztah anti-HMGCR protilátky k imunitně zprostředkované nekrotizující myopatii, její těsný vztah k předchozí léčbě statiny a recentní nárůst incidence. Prokázali jsme překvapivou negativní asociaci hladin IFNα s aktivitou svalového postižení na magnetické rezonanci, avšak popisujeme korelaci klinické aktivity onemocnění s aktivací dráhy interferonu typu I u pacientů s dermatomyozitidou. Dále jsme prokázali korelaci hladin resistinu a klinické aktivity a hladin visfatinu s klinickou aktivitou u anti-Jo-1 pozitivních pacientů. Resistin i visfatin jsou zvýšeně exprimované ve svalové tkáni pacientů s IIM. Navíc prokazujeme odlišnou specifickou expresi některých miRNA v sérech pacientů s PM a DM. Séra pacientů s IIM jsou schopna aktivace dráhy interferonu typu I in vitro a tato aktivace je zprostředkována především IFNα. Taktéž prokazujeme schopnost resistinu indukovat expresi prozánětlivých cytokinů (IL-1β, IL-6, MCP-1) v mononukleárních buňkách. Závěr. Naše výsledky ukazují na vztah jednotlivých molekul imunitního systému k jednotlivým podtypům či fenotypovým projevům IIM a demonstrují jejich význam v patogenezi tohoto onemocnění.
Introduction: Idiopathic inflammatory myopathies (myositis, IIM) are heterogeneous group of rare autoimmune systemic diseases, characterized particularly by proximal skeletal muscle weakness. Heretogeneity of myositis is based on different pathogenetic mechanisms which may be reflected by variable imunophenotypic response in individual subtypes. Objectives: The aim of this work was to explore the associations and influence of soluble factors of immune system in patients’ sera on phenotypic characteristics and subtypes of IIM, to describe their expression in inflamed muscle tissue and study their eventual role in pathogenesis by analysis of effect on immune and muscle cells in vitro. Results: We have described prevalence and characteristics of joint involvement in myositis patients and its significant association with anti-Jo-1 autoantibody. Furthermore, we confirmed the relation of anti-HMGCR antibody to immune mediated necrotizing myopathy, its tight relation to statins and recent increase in incidence. We showed inverse association of IFNα serum levels with muscle activity detected on MRI. Clinical activity positively correlated with IFN type-I pathway activation in patients with dermatomyositis. We also show positive correlation of resistin levels with clinical activity and correlation of activity with visfatin serum levels in anti-Jo-1 positive patients. Both resistin and visfatin are up-regulated in muscle tissues of IIM patients. Moreover, we showed differentially expressed characteristic miRNAs in sera of patients with PM and DM. Sera of patients with IIM are capable to activate IFN-type I pathway in vitro and IFNα seems to be responsible for that. We also demonstrate the ability of resistin to induce expression of pro-inflammatory cytokines (IL-1β, IL-l, MCP-1) in mononuclear cells. Conclusions: Our results show the relation of particular molecules of immune system to individual subtypes of IIM and their phenotypic manifestations and suggest the role of soluble mediators in pathogenesis of idiopathic inflammatory myopathies.
- MeSH
- autoprotilátky fyziologie klasifikace MeSH
- chemokiny fyziologie MeSH
- cytokininy fyziologie MeSH
- imunitní systém fyziologie patologie MeSH
- incidence MeSH
- interferony analýza MeSH
- klinické zkoušky jako téma MeSH
- lidé MeSH
- myozitida * diagnóza klasifikace patologie MeSH
- nemoci svalů MeSH
- resistin analýza MeSH
- rozpustnost MeSH
- vzácné nemoci MeSH
- Check Tag
- lidé MeSH
