Mineralization of hydrogel biomaterials with calcium phosphate (CaP) is considered advantageous for bone regeneration. Mineralization can be both induced by the enzyme alkaline phosphatase (ALP) and promoted by calcium-binding biomolecules, such as plant-derived polyphenols. In this study, ALP-loaded gellan gum (GG) hydrogels were enriched with gallotannins, a subclass of polyphenols. Five preparations were compared, namely three tannic acids of differing molecular weight (MW), pentagalloyl glucose (PGG), and a gallotannin-rich extract from mango kernel (Mangifera indica L.). Certain gallotannin preparations promoted mineralization to a greater degree than others. The various gallotannin preparations bound differently to ALP and influenced the size of aggregates of ALP, which may be related to ability to promote mineralization. Human osteoblast-like Saos-2 cells grew in eluate from mineralized hydrogels. Gallotannin incorporation impeded cell growth on hydrogels and did not impart antibacterial activity. In conclusion, gallotannin incorporation aided mineralization but reduced cytocompatibility.
- MeSH
- alkalická fosfatasa metabolismus MeSH
- antibakteriální látky farmakologie MeSH
- bakteriální polysacharidy MeSH
- biokompatibilní materiály MeSH
- biomimetika metody MeSH
- fosforečnany vápenaté MeSH
- fyziologická kalcifikace účinky léků MeSH
- hydrogely chemie MeSH
- hydrolyzovatelné taniny metabolismus farmakologie MeSH
- lidé MeSH
- Mangifera chemie MeSH
- minerály chemie MeSH
- osteoblasty metabolismus MeSH
- polyfenoly chemie MeSH
- polysacharidy chemie MeSH
- regenerace kostí MeSH
- rostlinné extrakty chemie MeSH
- rostliny metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Prospective, controlled clinical trials in postmenopausal osteoporosis typically compare effects of an active drug with placebo in addition to vitamin D and calcium supplementation in both treatment arms. While clinical benefits are documented, the effect of this supplementation in the placebo arm and in clinical practice on bone material composition properties is unknown. The purpose of the present study was to evaluate these bone quality indices (specifically mineral/matrix, nanoporosity, glycosaminoglycan content, mineral maturity/crystallinity, and pyridinoline content) in patients that either received long-term vitamin D (400-1200IU) and calcium (1.0-1.5g) supplementation, or did not. We have analyzed by Raman microspectroscopy the bone forming trabecular surfaces of iliac crest in pre-treatment samples of a teriparatide study and the endpoint biopsies of the control arm obtained from the HORIZON trial. In general, the mineral/matrix ratio and the glycosaminoglycan (GAG) content was higher while nanoporosity, (a surrogate for tissue water content), the mineral maturity/crystallinity (MMC) and the pyridinoline (Pyd) content was lower in patients without long-term supplementation. Moreover, all indices were significantly dependent on tissue age. In conclusion, vitamin D and calcium supplementation is associated with altered mineral and organic matrix properties.
- MeSH
- aminokyseliny metabolismus MeSH
- analýza rozptylu MeSH
- fyziologická kalcifikace účinky léků MeSH
- glykosaminoglykany metabolismus MeSH
- kostní matrix účinky léků metabolismus MeSH
- lidé MeSH
- nanočástice chemie MeSH
- poréznost MeSH
- postmenopauzální osteoporóza farmakoterapie patofyziologie MeSH
- potravní doplňky * MeSH
- Ramanova spektroskopie MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- vápník farmakologie terapeutické užití MeSH
- vitamin D farmakologie terapeutické užití MeSH
- Check Tag
- lidé MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Cortical bone, the dominant component of the human skeleton by volume, plays a key role in protecting bones from fracture. We analyzed the cortical bone effects of teriparatide treatment in postmenopausal women with osteoporosis who had previously received long-term alendronate (ALN) therapy or were treatment naïve (TN). Tetracycline-labeled paired iliac crest biopsies obtained from 29 ALN-pretreated and 16 TN women were evaluated for dynamic histomorphometric parameters of bone formation at the periosteal, endocortical and intracortical bone compartments, before and after 24months of teriparatide treatment. At baseline, the frequency of specimens without any endocortical and periosteal tetracycline labeling, and the percentage of quiescent osteons, was higher in the ALN than the TN group. Endocortical and periosteal mineralizing surface (MS/BS%), periosteal bone formation rate (BFR/BS), mineral apposition rate (MAR) and the number of intracortical forming osteons were significantly lower in the ALN-pretreated patients than in the TN group. Following teriparatide treatment, the frequency of endocortical and periosteal unlabeled biopsies decreased; in the ALN-pretreated group the percentage of quiescent osteons decreased and, in contrast, forming and resorbing osteons were increased. Teriparatide treatment resulted in significant increases of MAR in the endocortical, and MS/BS% in the periosteal compartment in the ALN-pretreated group. Most indices of bone formation remained lower in the ALN-pretreated group compared with the TN group at study end. Endocortical wall width was increased in both ALN-pretreated and TN groups. Cortical porosity and cortical thickness were significantly increased in the ALN-pretreated group after teriparatide treatment. Our results suggest that 24months of teriparatide treatment increases cortical bone formation and cortical turnover in patients who were either TN or had previous ALN therapy.
- MeSH
- alendronát farmakologie terapeutické užití MeSH
- fyziologická kalcifikace účinky léků MeSH
- lidé MeSH
- osteogeneze účinky léků MeSH
- postmenopauzální osteoporóza farmakoterapie patofyziologie MeSH
- senioři MeSH
- teriparatid farmakologie terapeutické užití MeSH
- Check Tag
- lidé MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- klinické zkoušky MeSH
- práce podpořená grantem MeSH
This article aims to share the results of research conducted in the Fergana chemical plant of furan compounds (FCPFC) in Uzbekistan.19 workers of the Furan compounds plant, in Fergana, Uzbekistan, were tested. By neutron activation analysis method, we have studied microelement composition of saliva, blood, dental hard tissue, and the level of Ca, Zn, Fe, and Ag in these subjects. We have detected that the level of chemical elements in dental hard tissue, blood, and saliva of these workers was subject to negative changes as compared to the analysis results from those in the control group. The research results have practical value for the prophylaxis, treatment, and health resumption of the people living in rugged ecological environment and workers who are engaged with harmful substances in chemical industry. Furthermore,this research also provides recommendations for treatment of dental diseases related to common conditions of pathophysiological processes carried out by living organisms.
- MeSH
- dentin chemie MeSH
- furany * škodlivé účinky MeSH
- fyziologická kalcifikace účinky léků MeSH
- lidé MeSH
- nemoci zubů etiologie prevence a kontrola MeSH
- neutronová aktivační analýza statistika a číselné údaje MeSH
- orální zdraví MeSH
- pracovní expozice * škodlivé účinky MeSH
- sliny chemie MeSH
- stopové prvky analýza krev MeSH
- stříbro analýza krev MeSH
- vápník analýza krev MeSH
- železo analýza krev MeSH
- zinek analýza krev MeSH
- zubní cement (tkáň) chemie MeSH
- zubní sklovina chemie MeSH
- Check Tag
- lidé MeSH
- MeSH
- chronické selhání ledvin * diagnóza etiologie komplikace MeSH
- dialýza škodlivé účinky MeSH
- fyziologická kalcifikace účinky léků MeSH
- hodnocení léčiv MeSH
- hyperfosfatemie farmakoterapie prevence a kontrola MeSH
- kostní denzita účinky léků MeSH
- lidé MeSH
- minerálová a kostní nemoc při chronickém onemocnění ledvin farmakoterapie patologie prevence a kontrola MeSH
- multicentrické studie jako téma MeSH
- naftaleny * aplikace a dávkování metabolismus škodlivé účinky terapeutické užití MeSH
- parathormon metabolismus škodlivé účinky MeSH
- receptory "calcium-sensing" MeSH
- receptory kalcitriolu fyziologie metabolismus MeSH
- sekundární hyperparatyreóza diagnóza etiologie terapie MeSH
- transplantace ledvin MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
- přehledy MeSH
- MeSH
- diagnostické techniky kardiovaskulární klasifikace využití MeSH
- echokardiografie metody využití MeSH
- fibrilace síní diagnóza etiologie chirurgie MeSH
- fyziologická kalcifikace fyziologie účinky léků MeSH
- kardiovaskulární chirurgické výkony metody využití MeSH
- komorbidita MeSH
- lidé MeSH
- senioři MeSH
- stenóza arteria carotis diagnóza chirurgie terapie MeSH
- tyreotoxikóza diagnóza etiologie chirurgie MeSH
- výsledek terapie MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- Publikační typ
- kazuistiky MeSH
- MeSH
- chronická nemoc MeSH
- denzitometrie metody statistika a číselné údaje využití MeSH
- fyziologická kalcifikace fyziologie účinky léků MeSH
- komorbidita MeSH
- kostní denzita fyziologie účinky léků MeSH
- lidé MeSH
- metabolické nemoci kostí diagnóza etiologie klasifikace MeSH
- nedostatek vitaminu D etiologie komplikace metabolismus MeSH
- osteomalacie diagnóza etiologie metabolismus MeSH
- osteoporóza diagnóza etiologie metabolismus MeSH
- pankreatitida diagnóza komplikace metabolismus MeSH
- stupeň závažnosti nemoci MeSH
- vitamin D farmakologie metabolismus terapeutické užití MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
The calcium consumption in Poland is too low and in some social groups reach up to 50% of recommended amounts only. The result of calcium shortage among adults is osteopenia or osteoporosis. The aim of the work was estimation of influence of Polish soldiers' daily food ration supplementation with 600 mg calcium on the mineralization status of the bones within 6 months. The calcium was delivered in the orange-tangerine nectar. Total of 203 young men serving in the air cavalry units underwent the examination. Based on the densitometric examinations of bones calcification it was found that half-year lasting supplementation profitably influenced on the bones density of examined soldiers. Significant increase of number of soldiers indicating standard bones density from 19,6% in the service beginning up to 37,3% after 6 months was found. At the same time number of soldiers indicating bones mineralization typical for osteopenia considerably decreased from 74,5% down to 49%.
- MeSH
- ergokalciferoly MeSH
- fyziologická kalcifikace účinky léků MeSH
- krysa rodu rattus MeSH
- páteř metabolismus účinky záření MeSH
- vápník metabolismus MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- zvířata MeSH
- Publikační typ
- srovnávací studie MeSH
