Ochratoxin A is a nephrotoxic and renal carcinogenic mycotoxin and is a common contaminant of various food commodities. Eighty six kinds of foodstuffs (1032 food samples) were collected in 2011-2013. High-performance liquid chromatography with fluorescence detection was used for ochratoxin A determination. Limit of quantification of the method varied between 0.01-0.2 μg/kg depending on the food matrices. The most exposed population is children aged 4-6 years old. Globally for this group, the maximum ochratoxin A dietary exposure for "average consumer" was estimated at 3.3 ng/kg bw/day (lower bound, considering the analytical values below the limit of quantification as 0) and 3.9 ng/kg bw/day (middle bound, considering the analytical values below the limit of quantification as 1/2 limit of quantification). Important sources of exposure for this latter group include grain-based products, confectionery, meat products and fruit juice. The dietary intake for "high consumers" in the group 4-6 years old was estimated from grains and grain-based products at 19.8 ng/kg bw/day (middle bound), from tea at 12.0 ng/kg bw/day (middle bound) and from confectionery at 6.5 ng/kg bw/day (middle bound). For men aged 18-59 years old beer was the main contributor with an intake of 2.60 ng/kg bw/day ("high consumers", middle bound). Tea and grain-based products were identified to be the main contributors for dietary exposure in women aged 18-59 years old. Coffee and wine were identified as a higher contributor of the OTA intake in the population group of women aged 18-59 years old compared to the other population groups.

• MeSH
• dítě MeSH
• dospělí MeSH
• jedlá semena mikrobiologie   MeSH
• káva mikrobiologie   MeSH
• kontaminace potravin analýza   MeSH
• lidé středního věku MeSH
• lidé MeSH
• masné výrobky MeSH
• mladiství MeSH
• mladý dospělý MeSH
• ochratoxiny aplikace a dávkování   analýza   toxicita   MeSH
• ovocné a zeleninové šťávy mikrobiologie   MeSH
• pivo mikrobiologie   MeSH
• populační skupiny * MeSH
• potravinářská mikrobiologie MeSH
• předškolní dítě MeSH
• víno mikrobiologie   MeSH
• Check Tag
• dítě MeSH
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• předškolní dítě MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Česká republika MeSH

Coffee is one of the world's most popular beverages and has been growing steadily in commercial importance. Nowadays, coffee is the second largest traded commodity in the world, after petroleum. Hence, coffee industry is responsible for the generation of large amounts of waste, especially spent coffee grounds (SCG). Various attempts to valorize this waste stream of coffee industry were made. This article summarizes our research and publications aiming at the conversion of SCG into valuable products - polyhydroxyalkanoates (PHAs) and carotenoids. At first, oil extracted from SCG (approx. 15 wt% oil in SCG) can be efficiently (YP/S=0.82 g/g) converted into PHA employing Cupriavidus necator H16. Further, the solid residues after oil extraction can be hydrolyzed (by the combination of chemical and enzymatic hydrolysis) yielding fermentable sugars, which can be further used as a substrate for the production of PHAs employing Bacillus megaterium (YP/S=0.04 g/g) or Burkholderia cepacia (YP/S=0.24 g/g). Alternatively, SCG hydrolysate can be used as a substrate for biotechnological production of carotenoids by carotenogenic yeast Sporobolomyces roseus. Solid residues after either oil extraction or hydrolysis can be used as fuel in industrial boilers to generate heat and energy. Therefore, entire biomass of SCG can be used for sustainable production of PHAs and/or carotenoids employing bio-refinery approach.

• MeSH
• biopaliva mikrobiologie   MeSH
• biotechnologie metody   MeSH
• extrakce kapalina-kapalina metody   MeSH
• karotenoidy biosyntéza   chemie   izolace a purifikace   MeSH
• káva chemie   mikrobiologie   MeSH
• odpadky - odstraňování metody   MeSH
• polyhydroxyalkanoáty biosyntéza   chemie   izolace a purifikace   MeSH
• zachování přírodních zdrojů metody   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH

Ochratoxin A (OTA) is nephrotoxic, hepatotoxic, immunotoxic, neurotoxic, reprotoxic, teratogenic, and carcinogenic (group 2B), being characterized by species and sex differences in sensitivity. Despite the fact that OTA is in some aspects a controversial topic, OTA is the most powerful renal carcinogen. The aim of this study was to make a small survey concerning OTA content in black tea, fruit tea, and ground roasted coffee, and to assess OTA transfer into beverages. OTA content was measured using a validated and accredited HPLC-FLD method with a limit of quantification (LOQ) of 0.35 ng/g. The OTA amount ranged from LOQ up to 250 ng/g in black tea and up to 104 ng/g in fruit tea. Black tea and fruit tea, naturally contaminated, were used to prepare tea infusions. The transfer from black tea to the infusion was 34.8% ± 1.3% and from fruit tea 4.1% ± 0.2%. Ground roasted coffee naturally contaminated at 0.92 ng/g was used to prepare seven kinds of coffee beverages. Depending on the type of process used, OTA transfer into coffee ranged from 22.3% to 66.1%. OTA intakes from fruit and black tea or coffee represent a non-negligible human source.

• MeSH
• čaj mikrobiologie   MeSH
• káva mikrobiologie   MeSH
• koncentrace vodíkových iontů MeSH
• kontaminace potravin analýza   MeSH
• ochratoxiny analýza   MeSH
• ovoce mikrobiologie   MeSH
• potravinářská mikrobiologie MeSH
• vysokoúčinná kapalinová chromatografie MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

By aid of a direct inoculation method it was followed the frequency of Aspergillus flavus - colony forming units in 11,526.5 p (100%) of 55 samples of some basic sensoric unchanged foodstuffs (cereal instant gruels 21%, milk powder 49%, pudding powders 7%, meal 9% and tea, coffee, cacao 12%) and inner contamination by A. flavus in 22,017 pieces of nuts and grains. It was found, that A. flavus colony forming units falls in Czechoslovak Socialistic Republic on the average on 7.2 g of the sample of crisp consistency (the least 0 colony/3.24%) of the investigated grains and nuts.

• MeSH
• Aspergillus flavus izolace a purifikace   MeSH
• čaj mikrobiologie   MeSH
• jedlá semena mikrobiologie   MeSH
• kakaovník mikrobiologie   MeSH
• káva mikrobiologie   MeSH
• mléko mikrobiologie   MeSH
• potravinářská mikrobiologie * MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Československo MeSH