- MeSH
- bakteriální pneumonie imunologie komplikace MeSH
- chřipka lidská epidemiologie imunologie komplikace MeSH
- epidemický výskyt choroby MeSH
- koinfekce epidemiologie imunologie MeSH
- kritický stav MeSH
- lidé MeSH
- pandemie MeSH
- pneumonie pneumokoková imunologie komplikace MeSH
- Streptococcus pneumoniae MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- přehledy MeSH
To examine evidence of positive antibodies against immunogenic proteins of Anaplasma phagocytophilum in patients with other tick-borne infections and to diagnose possible co-infections, 412 serum specimens were tested by immunoblotting using three specific Anaplasma antigens: surface proteins p44 and Asp62 and outer membrane protein A (OmpA). In total, 284 serum samples from children with Lyme borreliosis and 12 serum samples from children with tick-borne encephalitis were tested. Sera from patients with viral aseptic meningitis (n = 47) and from blood donors (n = 69) were used as controls. Among all serum specimens from patients with tick-borne infections submitted for this study, six samples (2·0%) showed positive IgM reactions and seven samples (2·4%) were IgG positive for A. phagocytophilum by immunoblot. Borderline reactivity was found in 30 samples (10·14%) for IgM and 36 samples (12·2%) for IgG. The difference between patients and blood donors was statistically significant for IgM (P = 0·006) and for IgG (P = 0·0007) antibodies. A statistically significant result was obtained for IgG (P = 0·02) but not for IgM between patients and children with aseptic meningitis. Immunoblot using three specific antigens provides novel information about the positivity of antibodies to A. phagocytophilum in children with other tick-borne infections. Taking into account clinical and laboratory findings of children despite antibody positivity, no case of human granulocytic anaplasmosis was demonstrated.
- MeSH
- Anaplasma phagocytophilum imunologie MeSH
- anaplasmóza diagnóza imunologie mikrobiologie MeSH
- bakteriální proteiny imunologie MeSH
- dítě MeSH
- klíšťová encefalitida imunologie mikrobiologie MeSH
- koinfekce diagnóza imunologie mikrobiologie MeSH
- lidé MeSH
- lymeská nemoc imunologie mikrobiologie MeSH
- membránové proteiny imunologie MeSH
- mladiství MeSH
- předškolní dítě MeSH
- proteiny vnější bakteriální membrány imunologie MeSH
- protilátky bakteriální krev MeSH
- western blotting MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- předškolní dítě MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
Interaction between pigs and Salmonella enterica serovar Derby (Salmonella Derby) is much less understood in comparison with Salmonella enterica serovar Typhimurium (Salmonella Typhimurium). To study interactions of weaned piglets with Salmonella Derby, we compared the course of infections with Salmonella Derby De1 and Salmonella Typhimurium DT104 strains, both isolated from pig herds with a long history of asymptomatic infection. Salmonella Derby strain used was shed during the 28-day experiment period, while Salmonella Typhimurium strain was not found in faeces after day 17 post-infection. When the piglets were co-infected with both strains, Salmonella Derby was present in faeces until the end of the experiment, whilst Salmonella Typhimurium disappeared after day 21 post-infection. At the end of the experiment, Salmonella Derby was present in more tissues when compared with Salmonella Typhimurium. Piglets infected with Salmonella Typhimurium responded earlier with synthesis of anti-lipopolysaccharide IgM and IgG antibodies and with higher antibody levels compared to piglets infected with Salmonella Derby. Cellular immune response to both strains was very low and was detected later than was the onset of IgG antibody production.
- MeSH
- feces mikrobiologie MeSH
- koinfekce imunologie MeSH
- nemoci prasat imunologie MeSH
- prasata MeSH
- protilátky bakteriální krev MeSH
- Salmonella enterica imunologie izolace a purifikace MeSH
- Salmonella typhimurium imunologie izolace a purifikace MeSH
- salmonelová infekce u zvířat imunologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The study established the frequency of detection of antigens and antibodies to HBV and HCV in mixed-hepatitis B C. The highest rates of detection of serological markers were observed in the identification in the blood the HBV genotype D and HCV genotype 1b. This phenomenon can be explained by the high replicative and antigenic activity of viruses in these genotypes, resulting in a high viral load in blood, compared with HBV genotypes A and C, HCV genotypes 3a and 2a. In this regard, the use of ELISA technique in combination with PCR diagnosis and genotyping of viruses is considered as desirable for the etiological interpretation of viral hepatitis. This approach also will enhance achieving the correct diagnosis and adequate choice of treatment programs
- Klíčová slova
- anti-HBs,
- MeSH
- biologické markery krev MeSH
- chronická hepatitida B * genetika imunologie MeSH
- chronická hepatitida C * genetika imunologie MeSH
- DNA virů MeSH
- ELISA statistika a číselné údaje MeSH
- genotyp MeSH
- Hepacivirus genetika imunologie MeSH
- hepatitida B - antigeny e genetika imunologie MeSH
- hepatitida B - antigeny jádrové genetika imunologie MeSH
- hepatitida B - antigeny povrchové genetika imunologie MeSH
- hepatitida C - antigeny genetika imunologie MeSH
- imunoglobulin G analýza imunologie MeSH
- imunoglobulin M analýza imunologie MeSH
- jaterní cirhóza patologie MeSH
- jehlová biopsie MeSH
- koinfekce * genetika imunologie MeSH
- lidé MeSH
- polymerázová řetězová reakce statistika a číselné údaje MeSH
- RNA virová MeSH
- virová nálož statistika a číselné údaje MeSH
- virové geny * MeSH
- virus hepatitidy B genetika imunologie MeSH
- Check Tag
- lidé MeSH
- Geografické názvy
- Uzbekistán MeSH
