X-ray photoelectron spectroscopy (XPS) Dotaz Zobrazit nápovědu
Radio frequency plasma is one of the means to modify the polymer surface namely in the activation of polypropylene membranes (PPM) with O2 plasma. Activated membranes were deposited with TiO2 nanoparticles by the dip coating method and the bare sample and modified sample (PPM5-TiO2) were irradiated by UV lamps for 20-120 min. Characterization techniques such as X-ray diffraction (XRD), Attenuated total reflection technique- Fourier transform infrared spectroscopy (ATR-FTIR), Thermogravimetric analysis (TGA), X-ray photoelectron spectroscopy (XPS), Scanning electron microscope (SEM) and water contact angle (WCA) measurements were applied to study the alteration of ensuing membrane surface properties which shows the nanoparticles on the sample surface including the presence of Ti on PPM. The WCA decreased from 135° (PPM) to 90° (PPM5-TiO2) and after UV irradiation, the WCA of PPM5-TiO2 diminished from 90° to 40°.
- MeSH
- difrakce rentgenového záření MeSH
- fotoelektronová spektroskopie MeSH
- kyslík chemie MeSH
- membrány umělé * MeSH
- nanočástice chemie MeSH
- plazmové plyny chemie MeSH
- polypropyleny chemie MeSH
- smáčivost MeSH
- spektroskopie infračervená s Fourierovou transformací MeSH
- teplota MeSH
- termogravimetrie MeSH
- titan chemie MeSH
- voda chemie MeSH
- Publikační typ
- časopisecké články MeSH
Although the nitrous oxide belongs among three of the most contributing greenhouse gases to global warming, it is quite neglected by photocatalytic society. The g-C3N4 and WO3 composites were therefore tested for the photocatalytic decomposition of N2O for the first time. The pure photocatalysts were prepared by simple calcination of precursors, and the composites were prepared by mixing of suspension of pure components in water followed by calcination. The structural (X-ray diffraction, X-ray photoelectron spectroscopy, high-resolution transmission electron microscopy), textural (N2 physisorption), and optical properties (diffuse reflectance spectroscopy, photoluminescence spectroscopy, photoelectrochemical measurements) of all composites were correlated with photocatalytic activity. The experimental results and results from characterization techniques confirmed creation of Z-scheme in the WO3/g-C3N4 composites, which was confirmed by hydroxyl radicals' trapping measurements. The photocatalytic decomposition of N2O was carried out in the presence of UVA light (peak intensity at 365 nm) and the 1:2 WO3/g-C3N4 composite was the most active one, but the photocatalytic activity was just negligibly higher than that of pure WO3. This is caused by relatively weak interaction between WO3 and g-C3N4 which was revealed from XPS.
The removal efficiency of heavy metal ions (cadmium(II), Cd(II); cobalt(II), Co(II); nickel(II), Ni(II); copper(II), Cu(II)) by potassium ferrate(VI) (K2FeO4, Fe(VI)) was studied as a function of added amount of Fe(VI) (or Fe) and varying pH. At pH = 6.6, the effective removal of Co(II), Ni(II), and Cu(II) from water was observed at a low Fe-to-heavy metal ion ratio (Fe/M(II) = 2:1) while a removal efficiency of 70% was seen for Cd(II) ions at a high Fe/Cd(II) weight ratio of 15:1. The role of ionic radius and metal valence state was explored by conducting similar removal experiments using Al(III) ions. The unique combination of X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), in-field Mössbauer spectroscopy, and magnetization measurements enabled the delineation of several distinct mechanisms for the Fe(VI)-prompted removal of metal ions. Under a Fe/M weight ratio of 5:1, Co(II), Ni(II), and Cu(II) were removed by the formation of MFe2O4 spinel phase and partially through their structural incorporation into octahedral positions of γ-Fe2O3 (maghemite) nanoparticles. In comparison, smaller sized Al(III) ions got incorporated easily into the tetrahedral positions of γ-Fe2O3 nanoparticles. In contrast, Cd(II) ions either did not form the spinel ferrite structure or were not incorporated into the lattic of iron(III) oxide phase due to the distinct electronic structure and ionic radius. Environmentally friendly removal of heavy metal ions at a much smaller dosage of Fe than those of commonly applied iron-containing coagulants and the formation of ferrimagnetic species preventing metal ions leaching back into the environment and allowing their magnetic separation are highlighted.
- MeSH
- chemické látky znečišťující vodu chemie izolace a purifikace MeSH
- čištění vody metody MeSH
- difrakce rentgenového záření MeSH
- fotoelektronová spektroskopie MeSH
- kationty chemie MeSH
- koncentrace vodíkových iontů MeSH
- magnetické nanočástice chemie MeSH
- sloučeniny draslíku chemie MeSH
- sloučeniny železa chemie MeSH
- spektroskopie Mossbauerova MeSH
- těžké kovy chemie izolace a purifikace MeSH
- voda MeSH
- železité sloučeniny chemie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
In the current study, well-defined polymer brushes are shown as an effective surface modification to resist the adhesion of whole blood and its components. Poly[oligo(ethylene glycol)methylether methacrylate] (poly(MeOEGMA)), poly(hydroxyethyl methacrylate) (poly(HEMA)), poly[N-(2-hydroxypropyl) methacrylamide] (poly(HPMA)), and poly(carboxybetaine acrylamide) (poly(CBAA)) brushes were grown by surface initiated atom transfer radical polymerization (SI-ATRP) and subsequently characterized by Fourier-transform infrared (FTIR) spectroscopy, X-ray photoelectron spectroscopy (XPS), dynamic contact angle measurements, atomic force microscopy (AFM), and surface plasmon resonance (SPR) spectroscopy. All brushes decreased the fouling from blood plasma over 95% and prevented the adhesion of platelets, erythrocytes, and leukocytes as evidenced by SPR and SEM measurements.
- MeSH
- biokompatibilní potahované materiály chemie MeSH
- bioznečištění MeSH
- buněčná adheze MeSH
- fotoelektronová spektroskopie MeSH
- krev metabolismus MeSH
- lidé MeSH
- mikroskopie atomárních sil MeSH
- plazma bohatá na destičky MeSH
- polymerizace MeSH
- polymery chemická syntéza chemie MeSH
- povrchové vlastnosti MeSH
- spektroskopie infračervená s Fourierovou transformací MeSH
- testování materiálů přístrojové vybavení MeSH
- voda MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
In this study, biodegradable poly(ε-caprolactone) (PCL) nanofibers (PCL-NF), collagen-coated PCL nanofibers (Col-c-PCL), and titanium dioxide-incorporated PCL (TiO2-i-PCL) nanofibers were prepared by electrospinning technique to study the surface and structural compatibility of these scaffolds for skin tisuue engineering. Collagen coating over the PCL nanofibers was done by electrospinning process. Morphology of PCL nanofibers in electrospinning was investigated at different voltages and at different concentrations of PCL. The morphology, interaction between different materials, surface property, and presence of TiO2 were studied by scanning electron microscopy (SEM), Fourier transform IR spectroscopy (FTIR), contact angle measurement, energy dispersion X-ray spectroscopy (EDX), and X-ray photoelectron spectroscopy (XPS). MTT assay and cell adhesion study were done to check biocompatibilty of these scaffolds. SEM study confirmed the formation of nanofibers without beads. FTIR proved presence of collagen on PCL scaffold, and contact angle study showed increment of hydrophilicity of Col-c-PCL and TiO2-i-PCL due to collagen coating and incorporation of TiO2, respectively. EDX and XPS studies revealed distribution of entrapped TiO2 at molecular level. MTT assay and cell adhesion study using L929 fibroblast cell line proved viability of cells with attachment of fibroblasts over the scaffold. Thus, in a nutshell, we can conclude from the outcomes of our investigational works that such composite can be considered as a tissue engineered construct for skin wound healing.
- MeSH
- biokompatibilní materiály chemie MeSH
- buněčná adheze fyziologie MeSH
- fibroblasty chemie MeSH
- fotoelektronová spektroskopie metody MeSH
- hydrofobní a hydrofilní interakce MeSH
- kolagen chemie MeSH
- kůže chemie MeSH
- nanovlákna chemie MeSH
- polyestery chemie MeSH
- povrchové vlastnosti MeSH
- titan chemie MeSH
- tkáňové inženýrství metody MeSH
- tkáňové podpůrné struktury MeSH
- Publikační typ
- časopisecké články MeSH
Argon plasma treatment was used to modify the surface of atelocollagen films using a plasmochemical reactor. To evaluate the effects of the treatment, the untreated and treated samples were characterized by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR), Scanning Electron Microscopy (SEM) imaging, and X-ray Photoelectron Spectroscopy (XPS) techniques. Cell growth was carried out by culturing human immortalized keratinocyte (HaCaT) cells and proliferation was measured via MTT assay. It was observed that argon plasma treatment significantly enhanced the extent of cell proliferation, which was ascribed to the favourable role of plasma treatment in inducing surface oxygen-containing entities together with increasing surface roughness. This can be considered as a potentially promising approach for tissue regeneration purposes.
- MeSH
- argon chemie MeSH
- buněčné kultury MeSH
- buněčné linie MeSH
- fotoelektronová spektroskopie MeSH
- keratinocyty cytologie fyziologie MeSH
- kolagen chemie MeSH
- lidé MeSH
- mikroskopie elektronová rastrovací MeSH
- proliferace buněk MeSH
- regenerace MeSH
- spektroskopie infračervená s Fourierovou transformací MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The aim of this study was to develop the potential tissue engineering applications of d-glucosamine (GlcN) immobilized onto the surface of a biodegradable matrix in order to induce a desired biological effect at biointerfaces. Thus, for sample preparation we used a novel multistep physicochemical approach. In the first step the poly(lactic acid) (PLA) films were exposed to a low pressure plasma in air atmosphere, followed by radical graft copolymerization with acrylic acid to yield a carboxyl-functionalized spacer layer on the PLA surface. The carboxyl groups were then coupled to GlcN molecules via the carbodiimide chemistry. The developed surfaces were characterized by X-ray Photoelectron Spectroscopy (XPS), Contact angle measurements and Atomic Force Microscopy (AFM). A preliminary study on the proliferation of fibroblasts on the developed surfaces was performed using the NIH/3T3 cell line.
- MeSH
- biokompatibilní materiály chemie MeSH
- fotoelektronová spektroskopie MeSH
- glukosamin chemie MeSH
- mikroskopie atomárních sil MeSH
- polyestery chemie MeSH
- povrchové vlastnosti MeSH
- proliferace buněk MeSH
- regenerace * MeSH
- smáčivost MeSH
- tkáňové inženýrství * MeSH
- Publikační typ
- časopisecké články MeSH
This paper investigates the interaction of human osteoblast-like Saos-2 cells with stainless steel covered by a film of densely inter-grown silicalite-1 crystals with defined outer and inner surfaces. The chemical composition of this film, labeled as SF(RT), was tuned by heat treatment at 300°C and 500°C (labeled as SF(300) and SF(500), respectively) and characterized by X-ray photoelectron spectroscopy (XPS), water drop contact angle (WCA) measurements and scanning electron microscopy (SEM). The number, the spreading area and the activity of alkaline phosphatase of human osteoblast-like Saos-2 cells in cultures on the silicalite-1 film were affected by the chemical composition of its outer surface and by its micro-porous structure. The number and the spreading area of the adhered osteoblast-like cells on day 1 was highest on the surface of SF(RT) relative to their adhesion and spreading on a glass cover slip due to the SF(RT) topology. However, SF(300) markedly supported cell growth during days 3 and 7 after seeding.
The preparation and thorough characterization of a hybrid magnetic carrier system for the possible transport of activated platinum-based anticancer drugs, as demonstrated for cisplatin (cis-[Pt(NH3)2Cl2], CDDP), are described. The final functionalized mag/Au-LA-CDDP* system consists of maghemite/gold nanoparticles (mag/Au) coated by lipoic acid (HLA; LA stands for deprotonated form of lipoic acid) and functionalized by activated cisplatin in the form of cis-[Pt(NH3)2(H2O)2]2+ (CDDP*). The relevant techniques (XPS, EDS, ICP-MS) proved the incorporation of the platinum-containing species on the surface of the studied hybrid system. HRTEM, TEM and SEM images showed the nanoparticles as spherical with an average size of 12 nm, while their superparamagnetic feature was proven by 57Fe Mössbauer spectroscopy. In the case of mag/Au, mag/Au-HLA and mag/Au-LA-CDDP*, weaker magnetic interactions among the Fe3+ centers of maghemite, as compared to maghemite nanoparticles (mag), were detected, which can be associated with the non-covalent coating of the maghemite surface by gold. The pH and time-dependent stability of the mag/Au-LA-CDDP* system in different media, represented by acetate (pH 5.0), phosphate (pH 7.0) and carbonate (pH 9.0) buffers and connected with the release of the platinum-containing species, showed the ability of CDDP* to be released from the functionalized nanosystem.
- MeSH
- biologický transport MeSH
- diferenční termická analýza MeSH
- fotoelektronová spektroskopie MeSH
- koncentrace vodíkových iontů MeSH
- kovové nanočástice chemie ultrastruktura MeSH
- magnetické jevy * MeSH
- nanotechnologie metody MeSH
- nosiče léků chemie MeSH
- platina terapeutické užití MeSH
- spektrofotometrie atomová MeSH
- spektrofotometrie infračervená MeSH
- spektrometrie rentgenová emisní MeSH
- spektroskopie Mossbauerova MeSH
- termogravimetrie MeSH
- železité sloučeniny chemie MeSH
- zlato chemie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Aim of this work is to study the interaction of krypton fluoride (KrF) excimer laser beam with a biopolymer and creation of new nanostructures with great potential for cell growth guidance. As a substrate we used biocompatible and biodegradable polymer polyhydroxybutyrate, which is frequently used in medicine and drug delivery system. Modification was carried out by KrF laser and method was also supplemented by treatment with Ar+plasma. The changes in physico-chemical properties of surface layer were determined by goniometry, gravimetry and X-ray photoelectron spectroscopy (XPS). Morphological changes and roughness were observed by atomic force microscopy (AFM) and scanning electron microscopy (SEM). Effect of laser treatment on the bulk material was studied by differential scanning calorimetry (DSC). Finally, the tests of mouse embryonic fibroblast (NIH 3T3) and human bone osteosarcoma (U-2 OS) cells' response was carried out on the selected samples. Modification of surface by laser with high number of pulses and fluence led to creation of surface layers with huge valleys and very high roughness. These structures were caused by extreme effect of ablation in combination with mass transfer. The results of the surface characterization will be useful for further research in the field of biopolymers structuring and modification, and may find a strong application in tissue engineering for single cell assays.
