Biological effects of manumycin-related compounds will be tested using a tissue culture models with human monocyte/macrophages (THP-1) and epithelial cell lines. For a screening of potential effect of tested compounds on pro-inflammatory cytokines and chemokines production, multiplex qRT-PCR will be used followed by measurement of proinflammatory cytokine concentrations, immunophenotypying and functional tests. Genetic screening will be used for identification of streptomycete strains producing manumycine-related compounds with consequent isolation and characterization of new compounds, construction of cosmid libraries, and genetic manipulations with the upper polyketide chain of manumycins (length, branching, saturation) including gene shuffling between producers.

Biologické účinky látek manumycinového typu budou testovány pomocí modelu tkáňových kultivací s lidskými liniemi monocytů/makrofágů (THP-1) a epiteliálních linií. Pro screening potenciálního efektu testovaných látek na tvorbu prozánětlivých cytokinů a chemokinů bude využita multiplexová kvantitativní RT-PCR s následným měřením koncentrací cytokinů, imunofenotypizací a funkčními testy. Kmeny streptomycet produkující manumycinové látky budou identifikovány pomocí genového screeningu, bude provedena izolace a charakterizace nových látek, konstrukce kosmidových knihoven a genetická manipulace horního polyketidového řetězce manumycinů (délka, štěpení, saturace) včetně genového "shufflingu" mezi producenty.

• MeSH
• Azithromycin MeSH
• Cytokines MeSH
• Epithelial Cells MeSH
• Genetic Techniques MeSH
• Immunophenotyping MeSH
• Immunomodulation MeSH
• Clarithromycin MeSH
• Cosmids MeSH
• Macrophages MeSH
• Reverse Transcriptase Polymerase Chain Reaction MeSH
• Streptomyces MeSH

• Conspectus
• Patologie. Klinická medicína
• NML Fields
• alergologie a imunologie
• biologie
• farmacie a farmakologie
• NML Publication type
• závěrečné zprávy o řešení grantu IGA MZ ČR

• MeSH
• Anti-Bacterial Agents pharmacokinetics   therapeutic use   MeSH
• Community-Acquired Infections drug therapy   MeSH
• Protein Synthesis Inhibitors MeSH
• Ketolides pharmacokinetics   pharmacology   metabolism   therapeutic use   MeSH
• Macrolides pharmacokinetics   pharmacology   metabolism   adverse effects   therapeutic use   MeSH
• RNA, Ribosomal, 23S MeSH
• Structure-Activity Relationship MeSH
• Publication type
• Collected Work MeSH

• Conspectus
• Farmacie. Farmakologie
• NML Fields
• farmacie a farmakologie

• MeSH
• Acute Disease therapy   MeSH
• Anti-Bacterial Agents economics   pharmacokinetics   pharmacology   therapeutic use   MeSH
• Drug Resistance, Bacterial MeSH
• Bronchitis drug therapy   MeSH
• Chronic Disease MeSH
• Fluoroquinolones therapeutic use   MeSH
• Protein Synthesis Inhibitors therapeutic use   MeSH
• Topoisomerase II Inhibitors therapeutic use   MeSH
• Clarithromycin administration & dosage   pharmacokinetics   therapeutic use   MeSH
• Drug Therapy, Combination MeSH
• Piperazines therapeutic use   MeSH
• Pneumococcal Infections drug therapy   MeSH
• Disease Progression MeSH
• Treatment Outcome MeSH
• Publication type
• Collected Work MeSH

• Conspectus
• Patologie. Klinická medicína
• Farmacie. Farmakologie
• NML Fields
• pneumologie a ftizeologie

• MeSH
• Anti-Infective Agents therapeutic use   MeSH
• Erythromycin therapeutic use   MeSH
• Penicillin G therapeutic use   MeSH
• Penicillins therapeutic use   MeSH
• Rheumatic Fever prevention & control   MeSH
• Streptococcal Infections drug therapy   MeSH
• Sulfadiazine therapeutic use   MeSH
• Sulfisoxazole therapeutic use   MeSH

• Conspectus
• Patologie. Klinická medicína
• NML Publication type
• publikace WHO

• MeSH
• Anti-Bacterial Agents MeSH
• Borrelia drug effects   MeSH
• Helicobacter pylori drug effects   MeSH
• Pharmacology, Clinical MeSH
• Leucomycins pharmacokinetics   pharmacology   therapeutic use   MeSH
• Macrolides MeSH
• Mycobacterium avium drug effects   MeSH
• Pneumocystis carinii drug effects   MeSH
• Roxithromycin pharmacokinetics   pharmacology   contraindications   adverse effects   therapeutic use   MeSH
• Toxoplasma drug effects   MeSH
• Publication type
• Collected Work MeSH

• Conspectus
• Farmacie. Farmakologie
• NML Fields
• farmacie a farmakologie
• farmakoterapie
• farmacie a farmakologie

• MeSH
• Azithromycin pharmacology   MeSH
• Opportunistic Infections drug therapy   MeSH
• Sexually Transmitted Diseases drug therapy   MeSH
• Publication type
• Congress MeSH
• Collected Work MeSH

• Conspectus
• Lékařské vědy. Lékařství
• NML Fields
• farmacie a farmakologie
• infekční lékařství
• dermatovenerologie

• MeSH
• Acne Vulgaris drug therapy   MeSH
• Erythromycin therapeutic use   MeSH
• Zinc Compounds therapeutic use   MeSH

• Conspectus
• Patologie. Klinická medicína
• NML Fields
• dermatovenerologie
• NML Publication type
• brožury

• MeSH
• Azithromycin pharmacology   therapeutic use   MeSH
• Antineoplastic Combined Chemotherapy Protocols MeSH
• Publication type
• Collected Work MeSH

• Conspectus
• Farmacie. Farmakologie
• NML Fields
• farmacie a farmakologie
• farmakoterapie

• MeSH
• Respiratory Tract Infections drug therapy   MeSH
• Roxithromycin pharmacology   MeSH

• Conspectus
• Farmacie. Farmakologie
• NML Fields
• farmacie a farmakologie

• MeSH
• Roxithromycin MeSH
• Publication type
• Congress MeSH

• Conspectus
• Farmacie. Farmakologie
• NML Fields
• farmacie a farmakologie