Electroencephalography (EEG) has been instrumental in epilepsy research for the past century, both for basic and translational studies. Its contributions have advanced our understanding of epilepsy, shedding light on the pathophysiology and functional organization of epileptic networks, and the mechanisms underlying seizures. Here we re-examine the historical significance, ongoing relevance, and future trajectories of EEG in epilepsy research. We describe traditional approaches to record brain electrical activity and discuss novel cutting-edge, large-scale techniques using micro-electrode arrays. Contemporary EEG studies explore brain potentials beyond the traditional Berger frequencies to uncover underexplored mechanisms operating at ultra-slow and high frequencies, which have proven valuable in understanding the principles of ictogenesis, epileptogenesis, and endogenous epileptogenicity. Integrating EEG with modern techniques such as optogenetics, chemogenetics, and imaging provides a more comprehensive understanding of epilepsy. EEG has become an integral element in a powerful suite of tools for capturing epileptic network dynamics across various temporal and spatial scales, ranging from rapid pathological synchronization to the long-term processes of epileptogenesis or seizure cycles. Advancements in EEG recording techniques parallel the application of sophisticated mathematical analyses and algorithms, significantly augmenting the information yield of EEG recordings. Beyond seizures and interictal activity, EEG has been instrumental in elucidating the mechanisms underlying epilepsy-related cognitive deficits and other comorbidities. Although EEG remains a cornerstone in epilepsy research, persistent challenges such as limited spatial resolution, artifacts, and the difficulty of long-term recording highlight the ongoing need for refinement. Despite these challenges, EEG continues to be a fundamental research tool, playing a central role in unraveling disease mechanisms and drug discovery.
- Klíčová slova
- EEG, analysis, animal models, genetic epilepsies, high‐frequency oscillations, mechanisms, preclinical,
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
Carp oedema virus (CEV) has distinct molecularly identified genogroups of viral mutations, denoted as I, IIa, and IIb. Failure to propagate CEV in vitro limits studies towards understanding its interactions with host cells. Here, virus isolates belonging to genogroup I collected during natural outbreaks in the Czech Republic were employed for routine CEV cultivation in monolayers of carp-derived primary cells, common carp brain (CCB) cells, and epithelioma papulosum cyprinid (EPC) cells. Induction of cytopathic effects (CPEs) was observed and recorded in affected cells. Cell survival rate was evaluated under serial dilutions of the CEV inoculum. Virus cell entry was quantified and visualized by qPCR and transmission electron microscopy, respectively. Study findings indicate primary gills epithelia likely present the most suitable matrix for CEV growth in vitro. Cells of the head kidney and spleen facilitate virus entry with microscopically confirmed CPEs and the presence of cytoplasmic pleomorphic virus particles. Cells of the trunk kidney and gonads are unlikely to permit virus cell entry and CPEs development. Although CEV cultivation in cell lines was inconclusive, EPC cells were CEV permissible. Monolayers of carp-derived primary cells show promise for CEV cultivation that could enable elaborate study of mechanisms underlying cellular binding and responses.
- Klíčová slova
- CEV, cultivation, cytopathy, gills epithelia, polymorphism,
- MeSH
- buněčné linie MeSH
- genotyp MeSH
- infekce vyvolané poxviry veterinární virologie MeSH
- kapři * virologie MeSH
- kultivace virů metody MeSH
- kultivované buňky MeSH
- nemoci ryb * virologie MeSH
- Poxviridae * fyziologie genetika MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
We have previously identified a unique disulfide bond in the crystal structure of Arabidopsis cytosolic seryl-tRNA synthetase involving cysteines evolutionarily conserved in all green plants. Here, we discovered that both cysteines are important for protein stability, but with opposite effects, and that their microenvironment may promote disulfide bond formation in oxidizing conditions. The crystal structure of the C244S mutant exhibited higher rigidity and an extensive network of noncovalent interactions correlating with its higher thermal stability. The activity of the wild-type showed resistance to oxidation with H2 O2 , while the activities of cysteine-to-serine mutants were impaired, indicating that the disulfide link may enable the protein to function under oxidative stress conditions which can be beneficial for an efficient plant stress response.
- Klíčová slova
- aminoacyl-tRNA synthetase, cysteine reactivity, disulfide bond, hydrogen peroxide, oxidative stress, thermal stability,
- MeSH
- Arabidopsis * genetika metabolismus MeSH
- cystein genetika metabolismus MeSH
- disulfidy MeSH
- oxidace-redukce MeSH
- rostliny metabolismus MeSH
- serin-tRNA-ligasa * chemie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- cystein MeSH
- disulfidy MeSH
- serin-tRNA-ligasa * MeSH
The Fischer-Tropsch heavy fraction is a potential feedstock for transport-fuels production through co-processing with fossil fuel fraction. However, there is still the need of developing new and green catalytic materials able to process this feedstock into valuable outputs. The present work studies the co-hydrocracking of the Fisher-Tropsch heavy fraction (FT-res.) with vacuum gas oil (VGO) at different ratios (FT-res. 9:1 VGO, FT-res. 7:3 VGO, and FT-res. 5:5 VGO) using phonolite-based catalysts (5Ni10W/Ph, 5Ni10Mo/Ph, and 5Co10Mo/Ph), paying attention to the overall conversion, yield, and selectivity of the products and properties. The co-processing experiments were carried out in an autoclave reactor at 450 °C, under 50 bars for 1 and 2 h. The phonolite-based catalysts were active in the hydrocracking of FT-res.:VGO mixtures, presenting different yields to gasoline, diesel, and jet fuel fractions, depending on the time of reaction and type of catalyst. Our results enable us to define the most suitable metal transition composition for the phonolite-based support as a hydrocracking catalyst.
- Klíčová slova
- Fischer–Tropsch wax, co-processing, hydrocracking, phonolite,
- Publikační typ
- časopisecké články MeSH
Membrane fouling is one of the main drawbacks encountered during the practical application of membrane separation processes. Cleaning of a membrane is important to reduce fouling and improve membrane performance. Accordingly, an effective cleaning method is currently of crucial importance for membrane separation processes in water treatment. To clean the fouling and improve the overall efficiency of membranes, deep research on the cleaning procedures is needed. So far, physical, chemical, or combination techniques have been used for membrane cleaning. In the current work, we critically reviewed the fouling mechanisms affecting factors of fouling such as the size of particle or solute; membrane microstructure; the interactions between membrane, solute, and solvent; and porosity of the membrane and also examined cleaning methods of microfiltration (MF) membranes such as physical cleaning and chemical cleaning. Herein, we mainly focused on the chemical cleaning process. Factors affecting the chemical cleaning performance, including cleaning time, the concentration of chemical cleaning, and temperature of the cleaning process, were discussed in detail. This review is carried out to enable a better understanding of the membrane cleaning process for an effective membrane separation process.
- Klíčová slova
- antifouling, chemical, cleaning, fouling, membrane, microfiltration,
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
Design and preparation of functional nanomaterials with specific properties requires precise control over their microscopic structure. A prototypical example is the self-assembly of diblock copolymers, which generate highly ordered structures controlled by three parameters: the chemical incompatibility between blocks, block size ratio and chain length. Recent advances in polymer synthesis have allowed for the preparation of gradient copolymers with controlled sequence chemistry, thus providing additional parameters to tailor their assembly. These are polydisperse monomer sequence, block size distribution and gradient strength. Here, we employ dissipative particle dynamics to describe the self-assembly of gradient copolymer melts with strong, intermediate, and weak gradient strength and compare their phase behavior to that of corresponding diblock copolymers. Gradient melts behave similarly when copolymers with a strong gradient are considered. Decreasing the gradient strength leads to the widening of the gyroid phase window, at the expense of cylindrical domains, and a remarkable extension of the lamellar phase. Finally, we show that weak gradient strength enhances chain packing in gyroid structures much more than in lamellar and cylindrical morphologies. Importantly, this work also provides a link between gradient copolymers morphology and parameters such as chemical incompatibility, chain length and monomer sequence as support for the rational design of these nanomaterials.
- Klíčová slova
- block copolymers, dissipative particle dynamics, gradient copolymers, gradient strength, microphase separation, nanomaterials, self-assembly,
- Publikační typ
- časopisecké články MeSH
