Artificial conditions limit the ability of laboratory studies to describe the complex effects of polluted environments on aquatic life. This study aimed to evaluate the impacts of treated wastewater discharge on the survival, growth, and sex ratio balance of the population of brown trout (Salmo trutta m. fario) in situ. Five floating incubators with 1000 eggs each were placed in the upstream reference and treated wastewater-affected sites in the Czech Republic for approximately three months. The hatched fish were grown in a natural environment for nearly one year. Water quality, including nutrients, temperature, pharmaceutical and personal care products, biological effects by bioassays and fish mortality, metabolic rate, and growth, were measured regularly. Up to 72 pharmaceutical and personal care products (7400-23000 ng/sampler) were detected in the passive samplers deployed downstream of the sewage treatment plant effluent. In vitro bioassays of the sampler extracts indicated elevated oestrogenic effects, transthyretin binding inhibition, and aryl hydrocarbon-mediated and androgenic potencies, showing endocrine-disrupting potential at the polluted site. The cumulated mortality of brown trout in the exposed group (9.67%) was significantly higher (p < 0.05) than in the control group (5.16%). In addition, the body size, growth, and metabolic rate of exposed fish were significantly lower (p < 0.05). The sex ratio of brown trout in the effluent-affected stretch was imbalanced, and sterile individuals were detected after several months of natural development in the stream. The observed effects of treated wastewater on the early developmental stages of aquatic wildlife could be connected to the development and readiness of adult individuals and, consequently, to the sustainability of freshwater ecosystems. Applying the hatching apparatus used in fishery practices, followed by comparing mortality, development, and sex with reference localities, seems to be a promising biomonitoring approach that can indicate hotspots for in-depth investigation and risk assessment.
- Klíčová slova
- Growth change, In situ exposure, Pharmaceutical and personal care products, Population sex ratio, Real exposure scenario, Sewage treatment plant effluent,
- Publikační typ
- časopisecké články MeSH
Some freshwater phytoplankton species have been suggested to produce estrogenic compounds in concentrations which could cause adverse effects to aquatic biota, while other studies showed no estrogenic effects after exposure to phytoplankton extracts or pointed out possible sources of the overestimation of the estrogenic activity. This study aimed to clarify these research inconsistencies by investigating estrogenicity of biomass extracts from both environmental freshwater blooms and laboratory cyanobacterial and algae cultures by in vitro reporter bioassay. Biomasses of 8 cyanobacterial and 3 algal species from 7 taxonomic orders were extracted and tested. Next to this, samples of environmental water blooms collected from 8 independent water bodies dominated by phytoplankton species previously assessed as laboratory cultures were tested. The results showed undetectable or low estrogenicity of both freshwater blooms and laboratory cultures with E2 equivalent concentration (EEQ) in a range from LOQ up to 4.5 ng EEQ/g of dry mass. Moreover, the co-exposure of biomass extracts with environmentally relevant concentration of model estrogen (steroid hormone 17β-estradiol; E2), commonly occurring in surface waters, showed simple additive interaction. However, some of the biomass extracts elicited partially anti-estrogenic effects in co-exposure with higher E2 concentration. In conclusion, our study documents undetectable or relatively low estrogenic potential of biomass extracts from both environmental freshwater blooms and studied laboratory cultured cyanobacterial and algae species. Nevertheless, in case of very high-density water blooms, even this low estrogenicity (detected for two cyanobacterial species) could lead to EEQ content in biomass reaching effect-based trigger values indicating potential risk, if recalculated per water volume at field sites. However, these levels would not occur in water under realistic environmental scenarios and the potential estrogenic effects would be most probably minor compared to other toxic effects caused by massive freshwater blooms of such high densities.
- Klíčová slova
- Anti-Estrogenicity, Aquatic ecosystem, Cyanobacteria, Estrogen, Freshwater bloom, Phytoplankton,
- MeSH
- estradiol toxicita MeSH
- estrogeny * toxicita analýza MeSH
- estron MeSH
- fytoplankton MeSH
- sinice * MeSH
- voda MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- estradiol MeSH
- estrogeny * MeSH
- estron MeSH
- voda MeSH
Monitoring methodologies reflecting the long-term quality and contamination of surface waters are needed to obtain a representative picture of pollution and identify risk drivers. This study sets a baseline for characterizing chemical pollution in the Danube River using an innovative approach, combining continuous three-months use of passive sampling technology with comprehensive chemical (747 chemicals) and bioanalytical (seven in vitro bioassays) assessment during the Joint Danube Survey (JDS4). This is one of the world's largest investigative surface-water monitoring efforts in the longest river in the European Union, which water after riverbank filtration is broadly used for drinking water production. Two types of passive samplers, silicone rubber (SR) sheets for hydrophobic compounds and AttractSPETM HLB disks for hydrophilic compounds, were deployed at nine sites for approximately 100 days. The Danube River pollution was dominated by industrial compounds in SR samplers and by industrial compounds together with pharmaceuticals and personal care products in HLB samplers. Comparison of the Estimated Environmental Concentrations with Predicted No-Effect Concentrations revealed that at the studied sites, at least one (SR) and 4-7 (HLB) compound(s) exceeded the risk quotient of 1. We also detected AhR-mediated activity, oxidative stress response, peroxisome proliferator-activated receptor gamma-mediated activity, estrogenic, androgenic, and anti-androgenic activities using in vitro bioassays. A significant portion of the AhR-mediated and estrogenic activities could be explained by detected analytes at several sites, while for the other bioassays and other sites, much of the activity remained unexplained. The effect-based trigger values for estrogenic and anti-androgenic activities were exceeded at some sites. The identified drivers of mixture in vitro effects deserve further attention in ecotoxicological and environmental pollution research. This novel approach using long-term passive sampling provides a representative benchmark of pollution and effect potentials of chemical mixtures for future water quality monitoring of the Danube River and other large water bodies.
- Klíčová slova
- Bioanalytical equivalent, Concentration addition, Iceberg modelling, Mixture effects, Passive sampling, Risk prioritization,
- MeSH
- antagonisté androgenů MeSH
- chemické látky znečišťující vodu * toxicita analýza MeSH
- ekotoxikologie MeSH
- estron MeSH
- kvalita vody * MeSH
- monitorování životního prostředí metody MeSH
- řeky chemie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antagonisté androgenů MeSH
- chemické látky znečišťující vodu * MeSH
- estron MeSH
Stagnant freshwaters can be affected by anthropogenic pollution and eutrophication that leads to massive growth of cyanobacteria and microalgae forming complex water blooms. These can produce various types of bioactive compounds, some of which may cause embryotoxicity, teratogenicity, endocrine disruption and impair animal or human health. This study focused on potential co-occurrence of estrogenic and retinoid-like activities in diverse stagnant freshwaters affected by phytoplankton blooms with varying taxonomic composition. Samples of phytoplankton bloom biomass and its surrounding water were collected from 17 independent stagnant water bodies in the Czech Republic and Hungary. Total estrogenic equivalents (EEQ) of the most potent samples reached up to 4.9 ng·g-1 dry mass (dm) of biomass extract and 2.99 ng·L-1 in surrounding water. Retinoic acid equivalent (REQ) measured by in vitro assay reached up to 3043 ng·g-1 dm in phytoplankton biomass and 1202 ng·L-1in surrounding water. Retinoid-like and estrogenic activities at some sites exceeded their PNEC and effect-based trigger values, respectively. The observed effects were not associated with any particular species of cyanobacteria or algae dominating the water blooms nor related to phytoplankton density. We found that taxonomically diverse phytoplankton communities can produce and release retinoid-like compounds to surrounding water, while estrogenic potency is likely related to estrogens of anthropogenic origin adsorbed to phytoplankton biomass. Retinoids occurring in water blooms are ubiquitous signalling molecules, which can affect development and neurogenesis. Selected water bloom samples (both water and biomass extracts) with retinoid-like activity caused effects on neurodifferentiation in vitro corresponding to those of equivalent all-trans-retinoic acid concentrations. Co-occurrence of estrogenic and retinoid-like activities in stagnant water bodies as well as the potential of compounds produced by water blooms to interfere with neural differentiation should be considered in the assessment of risks associated with water blooms, which can comprise complex mixtures of natural and anthropogenic bioactive compounds.
- Klíčová slova
- Endocrine disruption, Estrogenic activity, Neural differentiation, Phytoplankton water bloom, Retinoid-like activity,
- MeSH
- estrogeny analýza MeSH
- estron MeSH
- eutrofizace MeSH
- fytoplankton MeSH
- komplexní směsi MeSH
- lidé MeSH
- retinoidy * MeSH
- rostlinné extrakty MeSH
- sinice * MeSH
- tretinoin MeSH
- voda MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- estrogeny MeSH
- estron MeSH
- komplexní směsi MeSH
- retinoidy * MeSH
- rostlinné extrakty MeSH
- tretinoin MeSH
- voda MeSH
Complex mixtures of contaminants from multiple sources, including agriculture, industry or wastewater enter aquatic environments and might pose hazards or risks to humans or wildlife. Targeted analyses of a few priority substances provide limited information about water quality. In this study, a combined chemical and effect screening of water quality in the River Bosna, in Bosnia and Herzegovina was carried out, with focus on occurrence and effects of contaminants of emerging concern. Chemicals in water were sampled at 10 sites along the Bosna River by use of passive sampling. The combination of semipermeable membrane devices (SPMDs) and polar organic chemical integrative samplers (POCIS) enabled sampling of a broad range of contaminants from hydrophobic (PAHs, PCBs, OCPs) to hydrophilic compounds (pesticides, pharmaceuticals and hormones), which were determined by use of GC-MS and LC-MS (MS). In vitro, cell-based bioassays were applied to assess (anti)androgenic, estrogenic and dioxin-like potencies of extracts of the samplers. Of a total of 168 targeted compounds, 107 were detected at least once. Cumulative pollutant concentrations decreased downstream from the city of Sarajevo, which was identified as the major source of organic pollutants in the area. Responses in all bioassays were observed for samples from all sites. In general, estrogenicity could be well explained by analysis of target estrogens, while the drivers of the other observed effects remained largely unknown. Profiling of hazard quotients identified two sites downstream of Sarajevo as hotspots of biological potency. Risk assessment of detected compounds revealed, that 7 compounds (diazinon, diclofenac, 17β-estradiol, estrone, benzo[k]fluoranthene, fluoranthene and benzo[k]fluoranthene) might pose risks to aquatic biota in the Bosna River. The study brings unique results of a complex water quality assessment in a region with an insufficient water treatment infrastructure.
- Klíčová slova
- Contaminants of emerging concern - passive sampling, Hazard profiling - water quality monitoring, In vitro bioassay - endocrine disruption,
- MeSH
- biotest MeSH
- chemické látky znečišťující vodu analýza MeSH
- dioxiny analýza MeSH
- endokrinní disruptory analýza MeSH
- estrogeny analýza MeSH
- monitorování životního prostředí * MeSH
- odpadní voda MeSH
- organické látky analýza MeSH
- pesticidy analýza MeSH
- polychlorované bifenyly analýza MeSH
- polychlorované dibenzodioxiny analýza MeSH
- polycyklické aromatické uhlovodíky analýza MeSH
- řeky chemie MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Bosna a Hercegovina MeSH
- Názvy látek
- chemické látky znečišťující vodu MeSH
- dioxiny MeSH
- endokrinní disruptory MeSH
- estrogeny MeSH
- odpadní voda MeSH
- organické látky MeSH
- pesticidy MeSH
- polychlorované bifenyly MeSH
- polychlorované dibenzodioxiny MeSH
- polycyklické aromatické uhlovodíky MeSH
Growth inhibition of freshwater microalga Pseudokirchneriella subcapitata caused by a waste water treatment plant (WWTP) effluent extract was investigated using an effect directed analysis (EDA) approach. The objective was to identify compounds responsible for the toxicity by combining state-of-the-art sampling, bioanalytical, fractionation and non-target screening techniques. Three fractionation steps of the whole extract were performed and bioactive fractions were analysed with GC (xGC)-MS and LC-HRMS. In total, 383 compounds were tentatively identified, and their toxicity was characterized using US EPA Ecotox database, open scientific literature or modelled by ECOSAR. Among the top-ranking drivers of toxicity were pesticides and their transformation products, pharmaceuticals (barbiturate derivatives and macrolide antibiotics e.g. azithromycin), industrial compounds or caffeine and its metabolites. Several of the top-ranking pesticides are no longer registered for use in plant protection products or biocides in the Czech Republic (e.g. prometryn, atrazine, acetochlor, resmethrin) and some are approved only for use in biocides (e.g. terbutryn, carbendazim, phenothrin), which indicates that their non-agricultural input into aquatic environment via WWTPs should be carefully considered. The study demonstrated a functional strategy of combining biotesting, fractionation and non-target screening techniques in the EDA study focused on the identification of algal growth inhibitors in WWTP effluent.
- Klíčová slova
- Contaminants of emerging concern, Effect directed analysis, Fractionation, Non-target screening, Toxicity drivers,
- MeSH
- chemické látky znečišťující vodu analýza toxicita MeSH
- inhibitory růstu analýza toxicita MeSH
- mikrořasy účinky léků růst a vývoj MeSH
- monitorování životního prostředí metody MeSH
- odpadní voda chemie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- chemické látky znečišťující vodu MeSH
- inhibitory růstu MeSH
- odpadní voda MeSH
- Publikační typ
- časopisecké články MeSH
- tisková chyba MeSH
Growing concern about the adverse environmental and human health effects of a wide range of micropollutants requires the development of novel tools and approaches to enable holistic monitoring of their occurrence, fate and effects in the aquatic environment. A European-wide demonstration program (EDP) for effect-based monitoring of micropollutants in surface waters was carried out within the Marie Curie Initial Training Network EDA-EMERGE. The main objectives of the EDP were to apply a simplified protocol for effect-directed analysis, to link biological effects to target compounds and to estimate their risk to aquatic biota. Onsite large volume solid phase extraction of 50 L of surface water was performed at 18 sampling sites in four European river basins. Extracts were subjected to effect-based analysis (toxicity to algae, fish embryo toxicity, neurotoxicity, (anti-)estrogenicity, (anti-)androgenicity, glucocorticoid activity and thyroid activity), to target analysis (151 organic micropollutants) and to nontarget screening. The most pronounced effects were estrogenicity, toxicity to algae and fish embryo toxicity. In most bioassays, major portions of the observed effects could not be explained by target compounds, especially in case of androgenicity, glucocorticoid activity and fish embryo toxicity. Estrone and nonylphenoxyacetic acid were identified as the strongest contributors to estrogenicity, while herbicides, with a minor contribution from other micropollutants, were linked to the observed toxicity to algae. Fipronil and nonylphenol were partially responsible for the fish embryo toxicity. Within the EDP, 21 target compounds were prioritized on the basis of their frequency and extent of exceedance of predicted no effect concentrations. The EDP priority list included 6 compounds, which are already addressed by European legislation, and 15 micropollutants that may be important for future monitoring of surface waters. The study presents a novel simplified protocol for effect-based monitoring and draws a comprehensive picture of the surface water status across Europe.
- Klíčová slova
- Adverse effects, EDA-EMERGE, Environmental health, Human health, Large volume solid phase extraction, Simplified effect-directed analysis protocol,
- Publikační typ
- časopisecké články MeSH
The implementation of targeted and nontargeted chemical screening analysis in combination with in vitro and organism-level bioassays is a prerequisite for a more holistic monitoring of water quality in the future. For chemical analysis, little or no sample enrichment is often sufficient, while bioanalysis often requires larger sample volumes at a certain enrichment factor for conducting comprehensive bioassays on different endpoints or further effect-directed analysis (EDA). To avoid logistic and technical issues related to the storage and transport of large volumes of water, sampling would benefit greatly from onsite extraction. This study presents a novel onsite large volume solid phase extraction (LVSPE) device tailored to fulfill the requirements for the successful effect-based and chemical screening of water resources and complies with available international standards for automated sampling devices. Laboratory recovery experiments using 251 organic compounds in the log D range from -3.6 to 9.4 (at pH7.0) spiked into pristine water resulted in acceptable recoveries and from 60 to 123% for 159 out of 251 substances. Within a European-wide demonstration program, the LVSPE was able to enrich compounds in concentration ranges over three orders of magnitude (1ngL-1 to 2400ngL-1). It was possible to discriminate responsive samples from samples with no or only low effects in a set of six different bioassays (i.e. acetylcholinesterase and algal growth inhibition, androgenicity, estrogenicity, fish embryo toxicity, glucocorticoid activity). The LVSPE thus proved applicable for onsite extraction of sufficient amounts of water to investigate water quality thoroughly by means of chemical analysis and effect-based tools without the common limitations due to small sample volumes.
- Klíčová slova
- Applicability domain, Automated water sampler, Bioassay analysis, Chemical analysis, Effect-based analysis, LVSPE, Large-volume solid phase extraction,
- Publikační typ
- časopisecké články MeSH
The goal of this study was to contribute to understanding of the mechanisms behind sensitivity differences between early and late instar larvae of Chironomus riparius and to address the influence of the differences in standard testing approaches on the toxicity evaluation. A 10-day contact sediment toxicity test was carried out to assess sensitivity to cadmium exposure in relation to different age and laboratory culture line origin of test organisms. Chironomid larvae of early (OECD 218 method) and late instar (US-EPA600/R-99/064 method) differed substantially in sensitivity of traditional endpoints (OECD: LOEC 50 and 10 μg Cd/g dry weight (dw); US-EPA: LOEC > 1000 and 100 μg Cd/g dw for survival and growth, respectively). Bioaccumulated cadmium and metallothioneins (MTs) concentrations were analyzed to investigate the role of MTs in reduced sensitivity to cadmium in late instar larvae. Metallothioneins were induced after treatment to greater Cd concentrations, but their levels in relation to cadmium body burdens did not fully explain low sensitivity of late instars to cadmium, which indicates some other effective way of detoxification in late instars. This study brings new information related to the role of MTs in age-dependent toxicant sensitivity and discusses the implications of divergence in data generated by chironomid sediment toxicity tests by standardized methods using different instars.
- Klíčová slova
- Artificial sediment, Bioaccumulation, Cadmium, Chironomus, Metallothionein,
- MeSH
- Chironomidae účinky léků MeSH
- kadmium toxicita MeSH
- larva účinky léků MeSH
- metalothionein analýza metabolismus MeSH
- testy toxicity MeSH
- vystavení vlivu životního prostředí MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- kadmium MeSH
- metalothionein MeSH
