Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae are causative agents of the porcine respiratory disease complex. However, information on the prevalence of these pathogens in wild boars is scarce. This study aimed to investigate the presence of antibodies to Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae in wild boars in Serbia. In this study 253 serum samples from wild boars were tested for antibodies to Mycoplasma hyopneumoniae and Actinobacillus pleuropneumonia using the ELISA assay. The overall seroprevalence rates of Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae were 4.2% and 56.9%, respectively. Antibodies to both pathogens were detected in 20 sera samples (7.9%). The prevalence of wild boars that were seropositive for Mycoplasma hyopneumoniae differed with age and ranged from 10.7% to 33.3%, and for Actinobacillus pleuropneumoniae, it ranged from 51.8% to 83.3%. Wild boars are hard to control and are considered a high-risk infection source for outdoor and backyard pigs and eventually for commercial indoor farms as well. Thus, the result of this first serosurvey in Serbia should raise awareness of the importance of wild boars as potential reservoirs of bacterial pathogens such as Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae. Our data revealed the circulation of both pathogens in wild boars in Serbia, drawing attention to the potential health risk they present for domestic swine health.

• Klíčová slova
• ELISA, Serbia, antibodies, pneumonia, wild swine,
• Publikační typ
• časopisecké články MeSH

The objective was to expand and update the knowledge on the presence and genotype diversity of porcine circoviruses 2 and 3 (PCV2 and PCV3) in the wild boar populations from the hunting grounds in northeastern Serbia. The presence of PCV3 was not determined, and PCV2 was confirmed in 40.32% of the organ samples from 124 wild boars hunted from 2018 to 2019, indicating their significance in virus circulation since traditional pig farms with irregular PCV2 vaccination strategies are widespread in this region. The most prevalent genotype was PCV2d, followed by PCV2b and PCV2a in 55.6%, 38.9%, and 5.5% of the examined samples, respectively. Nucleotide sequences of the detected strains were homogenous within the genotype and clustered within the subgroups PCV2d-2, PCV2b-1A/B, and PCV2a-2D with high identity to European, Chinese, and Serbian domestic pig sequences suggesting their origin. Wild boars presented with no clinical or pathological signs of infection, implying that these animals might be less susceptible to disease, particularly since the cofactors present in pig farming systems that support the disease development are absent in the wild. The high PCV2 detection frequency demonstrates the importance of wildlife monitoring to track virus population dynamics, especially in regions with free-range pig farming in order to plan adequate disease control strategies.

• Klíčová slova
• PCR, PCV2, PCV3, Sus scrofa, phylogenetic analysis, sequencing,
• Publikační typ
• časopisecké články MeSH