The results of ELISA reactions made on the entire area of the plates, in the same way in all wells revealed that it is essential to eliminate the marginal rows. Even then there are in some instances deviations from the mean. These deviations frequently follow a loop-sided or dish-like pattern with the minimum in the centre of the plate. The deviations equalled in different wells on average 5-10% (or 20%) of the optic density. Between individual rows there may be a 0.100 difference in optic density, in rare instances as much as 0.300. These differences may influence the results during dilution of sera in a series more detailed than 4n and also when examining a single dilution of sera repeated in an inadequate number of wells.

• MeSH
• ELISA přístrojové vybavení   MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH

The enzymoimmunologic (ELISA) method was used to diagnose spring viremia in carp. The specific antibodies to Rhabdovirus carpio in the fish sera were detected by this method. Reaction specificity was tested by the use of viral and control antigens, by examination of positive and negative sera and by serum-free control incubation. Applying the ELISA method, the antiviral antibodies can be detected after infection or vaccination: this method is therefore suitable for thorough studies and when choosing the appropriate treatments and preventive measures.

• MeSH
• ELISA MeSH
• nemoci ryb diagnóza   MeSH
• protilátky virové analýza   MeSH
• Rhabdoviridae imunologie   MeSH
• ryby MeSH
• viremie diagnóza   veterinární   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• Názvy látek
• protilátky virové MeSH

Sensitive and high capacity ELISA techniques introduced for the detection of toxoplasmatic antibodies so far do not correlate very well with standard serological examination systems used in common diagnosis of Toxoplasmosis. The most frequently used method in the CSSR - SFT using tween-ether soluble antigen extract, and displaying very satisfactory agreement with the Sabin-Feldman test, was compared with the system of direct ELISA, using the same antigen extract and the described c-ELISA method. The latter method uses for antibody detection whole non-disintegrated formol-treated trophozoites of Toxoplasma gondii and thus in the reaction in particular membrane antigens, incl. latent ones, are involved. Parallel examination of 160 sera of subjects with clinical suspicion of toxoplasma infection by the standard CFT, method and methods ELISA and c-ELISA revealed a better correlation between CFT and c-ELISA (r = 0.8031) than between CFT and ELISA with soluble antigen (r = 0.6016). Other positive specific features of the c-ELISA method are the use of commercially produced antigen for the immunofluorescence test, considerable elimination of selective adsorption of the polystyrene carrier and the perspective of the long-term preservation of plates with the fixed antigen.

• MeSH
• ELISA * MeSH
• metody MeSH
• protilátky protozoální analýza   MeSH
• Toxoplasma imunologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• Názvy látek
• protilátky protozoální MeSH

Antiphospholipid antibodies (APA) are autoantibodies to negatively charged phospholipids such as a cardiolipin, phosphatidylserine and phosphatidylinositol. The occurrence of APA is associated with arterial and venous thromboembolic manifestations, thrombocytopenia and recurrent fetal loss. To date most studies have concentrated on antibodies to cardiolipin specifically. In this study we present own modification of the ELISA method for assessment of antiphosphatidylinositol antibodies (API). The phosphatidylinositol isolated from small green peas was used as an antigen. To test ELISA method 151 serum samples of patients with various diseases and 22 serum samples of blood donors were examined and the results were compared with the results of anticardiolipin antibodies (ACA). The positivity of API was 22.5% and ACA 20.5% in a group of patients. The simultaneous of both types of APA was found in 15.2%.

• MeSH
• antifosfolipidové protilátky analýza   MeSH
• antikardiolipinové protilátky analýza   MeSH
• ELISA metody   MeSH
• fosfatidylinositoly imunologie   MeSH
• lidé MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antifosfolipidové protilátky MeSH
• antikardiolipinové protilátky MeSH
• fosfatidylinositoly MeSH

The authors compare two ELISA methods for the assessment of antiphospholipid antibodies, classes IgG and IgM, in serum: ELISA Pin Plate System ALPHA DIALAB Co. and the ELISA method developed in the Research Institute of Rheumatic Diseases. Both methods use cardiolipin as antigen. In the Pin Plate test the immunochemical reaction antigen/antibody does not take place at the surface of the pits of the microtitration plates but on the tip of the next plate. The results of examinations of antiphospholipid antibodies obtained by the tested methods are comparable, the Pin Plate test is quicker and more sensitive, but its price limits routine use.

• MeSH
• antifosfolipidové protilátky krev   MeSH
• ELISA * MeSH
• imunoglobulin G analýza   MeSH
• imunoglobulin M analýza   MeSH
• lidé MeSH
• ženská infertilita imunologie   MeSH
• Check Tag
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• Názvy látek
• antifosfolipidové protilátky MeSH
• imunoglobulin G MeSH
• imunoglobulin M MeSH

The author describes the use of polyclonal antibodies against gentamicin by competitive ELISA. The author used polystyrene microplates as the solid phase for antibodies. Gentamicin was marked by horse radish peroxidase and a known amount of thus labelled gentamicin competed for a bond with an antibody with an unknown amount of gentamicin in serum. The method requires 10 microliters non-diluted serum and the results can be evaluated within one hour. The accuracy of the method according to values of the coefficient of variation (CV) is 9.0-13.2% in different tests and 5.2 to 9.2% within one examination.

• MeSH
• ELISA metody   MeSH
• gentamiciny krev   MeSH
• lidé MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• Názvy látek
• gentamiciny MeSH

The indirect fluorescent antibody test (IFAT) was compared with the enzyme-linked immunosorbent assay (ELISA) for the detection of specific antibodies to Sarcocystis sp. A set of 275 ovine blood samples was examined by both reactions. Cystozoites of Sarcocystis gigantea were used as the corpuscular antigen for the IFAT. For the diagnostics of sarcocystosis by the ELISA technique used the sandwich test of the antibody titration with a soluble antigen which was also prepared from S. gigantea macrocysts. Our studies confirmed that this antigen did not cross-react with Toxoplasma gondii. Titre 40 was determined as the limit one for the IFAT and titre 80 for the ELISA; which was confirmed by the direct detection of cysts in the muscles (Svobodová, 1989). The results of both methods are shown in Table I. 76.7% of the blood samples reacted positively in the IFAT and 83.6% in the ELISA. These methods were found to be suitable and can be utilized for the intravital routine diagnostics.

• MeSH
• ELISA veterinární   MeSH
• fluorescenční protilátková technika veterinární   MeSH
• nemoci ovcí diagnóza   MeSH
• ovce MeSH
• sarkocystóza diagnóza   veterinární   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH

The described ELISA reaction is suggested as a method for examination of antitoxoplasmatic IgG antibodies. It is a simple, i.e. three-layer sandwich with the following layers: 1. antigen 2. the examined serum either in serial dilution or single dilution 1:300 and 3. a peroxidase anti-IgG conjugate with a final conclusive step, i.e. a substrate solution with OPD. Sera and conjugate are allowed to bind for 30 mins. at 37 degrees C and the substrate solution with OPD is allowed to act for 15 mins., also at 37 degrees C, and thus the entire reaction lasts about two and half hours. The authors describe alternative procedures with standard serum of a known number of international units (I.U.) or without it, evaluated optically or by a photometer. In the version with standard serum evaluated optically we compare the different localization of equally stained wells in the series of serially diluted (4n) examined and standard serum and thus we assess, based on the different dilutions the number of I.U. in the examined serum. If we compare the sera with the serum standard by means of a photometer, we examine them in a single dilution of 1:300 and compare the resulting optical density (OD) with the calibration curve made with four dilutions of the standard. The calibration curve is plotted on a scale which straightens it approximately at an angle close to the optimal 45 degrees and which makes it possible to take readings of interpolated international units.(ABSTRACT TRUNCATED AT 250 WORDS)

• MeSH
• ELISA * metody   MeSH
• imunoglobulin G analýza   MeSH
• protilátky protozoální analýza   MeSH
• Toxoplasma imunologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• Názvy látek
• imunoglobulin G MeSH
• protilátky protozoální MeSH

Antiphospholipid antibodies (APA) are a group of antibodies against various phospholipid antigens. In order to extend the spectrum of examined specificities of antiphospholipid antibodies the authors elaborated an ELISA method for assessment of antiphosphatidyl serine antibodies (APSA). As antigen they used phosphatidyl serine isolated from the white matter of cattle brain. The ELISA method was tested by examining APSA in 12 patients with rheumatic diseases, 24 women with reproductive disorders and 50 patients with testicular tumours and the results were compared with examinations of anticardiolipin antibodies. The concurrent presence of both types of antibodies was recorded in 20.8% women with reproductive disorders and in 14% of the patients with testicular tumours. In these groups antiphosphatidyl serine antibodies were found more frequently.

• MeSH
• antifosfolipidové protilátky analýza   MeSH
• ELISA * MeSH
• fosfatidylseriny imunologie   MeSH
• lidé MeSH
• revmatické nemoci imunologie   MeSH
• skot MeSH
• testikulární nádory imunologie   MeSH
• ženská infertilita imunologie   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• skot MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• Názvy látek
• antifosfolipidové protilátky MeSH
• fosfatidylseriny MeSH

Seven calves of the Bohemian Spotted and Black-Pied Lowland breeds, aged two to three months and a half, were experimentally invaded per os with 5 000, 10 000, 25 000, 70 000, 95 000, 113 000 and 120 000 eggs of Taenia saginata. In the calf invaded with 70 000 eggs, antibodies were detected by the ELISA method already a week from invasion and in the remaining calves two to three weeks from invasion. The increase in the content of antibodies in the blood serum of the invaded animals was statistically significant from the fourth to the tenth week from invasion when the titre was 1 : 160 to 1 : 320 and when the measured extinction values ranged from 0.53 to 0.58. The antibody level remained significant and positive for seven months and a half to nine months. Clinical symptoms were observed in the calves invaded with 25 000 to 120 000 eggs for a period of 7 to 20 days from the 10th to 11th or 19th day after invasion. In the remaining calves the clinical symptoms were not significant. The post-mortal examination of four calves showed 2.1 to 3.9% of cysticerci from the applied eggs. The ELISA enzymatically immunological method, which was used for the first time in the Czech Socialist Republic in the case of cysticercosis, is suitable for detecting antibodies to the parasite Cysticercus bovis in the blood serum of calves experimentally invaded with the eggs of the tapeworm Taenia saginata.

• MeSH
• cysticerkóza diagnóza   veterinární   MeSH
• ELISA * MeSH
• imunoenzymatické techniky * MeSH
• nemoci skotu diagnóza   MeSH
• skot MeSH
• zvířata MeSH
• Check Tag
• skot MeSH
• zvířata MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH