BACKGROUND: Although several studies have investigated temperature-related mortality and morbidity, only a little is known about the short-term effects of temperature on ambulance dispatches. We aimed to conduct the first nationwide analysis of the association between temperatures and ambulance dispatches in Europe, including, for the first time, a detailed description of age-specific risks for 10-year age groups. METHODS: We collected daily data on ambulance dispatches and climate (i.e. temperature and relative humidity) for each district of Czechia (n = 77) during 2010-19. We estimated the relationship for each district by using a quasi-Poisson regression with distributed lag non-linear models. We then applied a multilevel multivariate random-effects meta-analysis to derive regional and countrywide average associations and calculated the burden of ambulance dispatches that was attributable to non-optimum temperatures. RESULTS: The susceptibility to low (high) temperatures increased (decreased) with age, except for the youth (<20 years), for whom the risks for both heat and cold were the highest. High temperatures contributed slightly to the risk of ambulance dispatches due to respiratory and cardiovascular causes, while the contribution of low temperatures was substantial. The overall ambulance dispatches burden that was attributable to non-optimum temperatures (optimum temperature = 7.9°C) was 3.55% (95% eCI: 3.43 to 3.67), with a predominant contribution of heat [2.32% (95% eCI: 2.15 to 2.46)] compared with cold [1.23% (95% eCI: 1.16 to 1.30)]. CONCLUSION: This data can be used as an early-warning indicator for temperature impacts, especially among vulnerable population subgroups, such as children, adolescents, and young adults. This evidence has important implications for healthcare system preparedness and management, and for the projections of climate change health impacts.

• Klíčová slova
• ambulance, climate change, cold, emergency, heat, heat waves, temperature,
• MeSH
• dítě MeSH
• dospělí MeSH
• kojenec MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mladý dospělý MeSH
• nízká teplota * škodlivé účinky   MeSH
• novorozenec MeSH
• předškolní dítě MeSH
• sanitky * statistika a číselné údaje   MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• teplota * MeSH
• věkové faktory MeSH
• vysoká teplota * škodlivé účinky   MeSH
• Check Tag
• dítě MeSH
• dospělí MeSH
• kojenec MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• novorozenec MeSH
• předškolní dítě MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Česká republika epidemiologie   MeSH

BACKGROUND: The association between nonoptimal temperatures and cardiovascular mortality risk is recognized. However, a comprehensive global assessment of this burden is lacking. OBJECTIVES: The goal of this study was to assess global cardiovascular mortality burden attributable to nonoptimal temperatures and investigate spatiotemporal trends. METHODS: Using daily cardiovascular deaths and temperature data from 32 countries, a 3-stage analytical approach was applied. First, location-specific temperature-mortality associations were estimated, considering nonlinearity and delayed effects. Second, a multivariate meta-regression model was developed between location-specific effect estimates and 5 meta-predictors. Third, cardiovascular deaths associated with nonoptimal, cold, and hot temperatures for each global grid (55 km × 55 km resolution) were estimated, and temporal trends from 2000 to 2019 were explored. RESULTS: Globally, 1,801,513 (95% empirical CI: 1,526,632-2,202,831) annual cardiovascular deaths were associated with nonoptimal temperatures, constituting 8.86% (95% empirical CI: 7.51%-12.32%) of total cardiovascular mortality corresponding to 26 deaths per 100,000 population. Cold-related deaths accounted for 8.20% (95% empirical CI: 6.74%-11.57%), whereas heat-related deaths accounted for 0.66% (95% empirical CI: 0.49%-0.98%). The mortality burden varied significantly across regions, with the highest excess mortality rates observed in Central Asia and Eastern Europe. From 2000 to 2019, cold-related excess death ratios decreased, while heat-related ratios increased, resulting in an overall decline in temperature-related deaths. Southeastern Asia, Sub-Saharan Africa, and Oceania observed the greatest reduction, while Southern Asia experienced an increase. The Americas and several regions in Asia and Europe displayed fluctuating temporal patterns. CONCLUSIONS: Nonoptimal temperatures substantially contribute to cardiovascular mortality, with heterogeneous spatiotemporal patterns. Effective mitigation and adaptation strategies are crucial, especially given the increasing heat-related cardiovascular deaths amid climate change.

• Klíčová slova
• Global Burden of Disease, cardiovascular death, death ratio, excess death, nonoptimal temperatures,
• MeSH
• celosvětové zdraví * MeSH
• kardiovaskulární nemoci * mortalita   MeSH
• lidé MeSH
• nízká teplota škodlivé účinky   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

Winter provides many challenges for terrestrial arthropods, including low temperatures and decreased food availability. Most arthropods are dormant in the winter and resume activity when conditions are favorable, but a select few species remain active during winter. Winter activity is thought to provide a head start on spring growth and reproduction, but few studies have explicitly tested this idea or investigated tradeoffs associated with winter activity. Here, we detail biochemical changes in overwintering winter-active wolf spiders, Schizocosa stridulans, to test the hypothesis that winter activity promotes growth and energy balance. We also quantified levels of putative cryoprotectants throughout winter to test the prediction that winter activity is incompatible with biochemical adaptations for coping with extreme cold. Body mass of juveniles increased 3.5-fold across winter, providing empirical evidence that winter activity promotes growth and therefore advancement of spring reproduction. While spiders maintained protein content throughout most of the winter, lipid content decreased steadily, suggesting either a lack of available prey to maintain lipids, or more likely, an allometric shift in body composition as spiders grew larger. Carbohydrate content showed no clear seasonal trend but also tended to be higher at the beginning of the winter. Finally, we tested the hypothesis that winter activity is incompatible with cryoprotectant accumulation. However, we observed accumulation of glycerol, myo-inositol, and several other cryoprotectants, although levels were lower than those typically observed in overwintering arthropods. Together, our results indicate that winter-active wolf spiders grow during the winter, and while cryoprotectant accumulation was observed in the winter, the modest levels relative to other species could make them susceptible to extreme winter events.

• Klíčová slova
• Cryoprotectants, Energy stores, Winter activity, Wolf spiders,
• MeSH
• energetický metabolismus * MeSH
• fyziologická adaptace MeSH
• glycerol analýza   MeSH
• index tělesné hmotnosti MeSH
• inositol analýza   MeSH
• kryoprotektivní látky analýza   MeSH
• lipidy analýza   MeSH
• nízká teplota škodlivé účinky   MeSH
• pavouci růst a vývoj   metabolismus   MeSH
• predátorské chování MeSH
• proteiny analýza   MeSH
• roční období MeSH
• rozmnožování MeSH
• sacharidy analýza   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Research Support, U.S. Gov't, Non-P.H.S. MeSH
• Názvy látek
• glycerol MeSH
• inositol MeSH
• kryoprotektivní látky MeSH
• lipidy MeSH
• proteiny MeSH
• sacharidy MeSH

The purpose of the present study was to assess the effect of different water immersion temperatures on handgrip performance and haemodynamic changes in the forearm flexors of males and females. Twenty-nine rock-climbers performed three repeated intermittent handgrip contractions to failure with 20 min recovery on three separate laboratory visits. For each visit, a randomly assigned recovery strategy was applied: cold water immersion (CWI) at 8 °C (CW8), 15 °C (CW15) or passive recovery (PAS). While handgrip performance significantly decreased in the subsequent trials for the PAS (p < 0.05), there was a significant increase in time to failure for the second and third trial for CW15 and in the second trial for CW8; males having greater performance improvement (44%) after CW15 than females (26%). The results indicate that CW15 was a more tolerable and effective recovery strategy than CW8 and the same CWI protocol may lead to different recovery in males and females.

• Klíčová slova
• Forearm, Haemoglobin, NIRS, Recovery, Tissue oxygenation,
• MeSH
• cvičení fyziologie   MeSH
• dospělí MeSH
• kryoterapie metody   MeSH
• kyslík fyziologie   MeSH
• lidé MeSH
• nízká teplota škodlivé účinky   MeSH
• ponoření MeSH
• sexuální faktory MeSH
• síla ruky fyziologie   MeSH
• svalová kontrakce fyziologie   MeSH
• voda MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• randomizované kontrolované studie MeSH
• Názvy látek
• kyslík MeSH
• voda MeSH

The transport of end-of-lay hens for slaughter presents a particular cause for concern in relation to hen welfare due to their less robust condition. During the period of 1 January 2010 to 31 December 2017, 17,436,074 end-of-lay hens transported for slaughter in 3,144 consignments were monitored, i.e., all hens transported from Czech farms to slaughterhouses in the Czech Republic in the monitored period. The overall mortality of hens during transport for slaughter was 0.516%. A significant (P < 0.05) impact of outside temperature and month of the year on transport-related mortality was found. Lower outside temperature was connected with the increased hen mortality and vice versa. Correspondingly, more deaths of end-of-lay hens occurred in the cold winter months of January (0.717%) and December (0.695%); on the contrary, the lowest death rates were recorded in August (0.364%). Differences were also found when comparing transport-related mortality rates according to the transport distance. The lowest mortality (0.338%) was found in hens transported for distances up to 50 km; longer distances were associated with increasing (P < 0.05) death rates, with the greatest losses (0.801%) recorded for distances from 201 to 300 km. These findings document the need for increased care for end-of-lay hens during their transport for slaughter in the winter at lower outside transport temperatures, in particular below 0°C (e.g., by adequate temperature regulation in the means of transport), and of hens transported over longer distances (if the transport distance cannot be reduced).

• Klíčová slova
• distance, hen, mortality, temperature, transport,
• MeSH
• chov zvířat MeSH
• dlouhověkost * MeSH
• doprava * MeSH
• jatka * MeSH
• kur domácí * MeSH
• nízká teplota škodlivé účinky   MeSH
• pohoda zvířat * MeSH
• psychologický odstup MeSH
• roční období MeSH
• zvířata MeSH
• Check Tag
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Česká republika MeSH

Mechanistic understanding about the nature of cellular cryoinjury and mechanisms by which some animals survive freezing while others do not is currently lacking. Here, we exploited the broadly manipulable freeze tolerance of larval malt flies (Chymomyza costata) to uncover cell and tissue morphological changes associated with freeze mortality. Diapause induction, cold acclimation and dietary proline supplementation generate malt fly variants ranging from weakly to extremely freeze tolerant. Using confocal microscopy and immunostaining of the fat body, Malpighian tubules and anterior midgut, we described tissue and cytoskeletal (F-actin and α-tubulin) morphologies among these variants after exposure to various cold stresses (from chilling at -5°C to extreme freezing at -196°C), and upon recovery from cold exposure. Fat body tissue appeared to be the most susceptible to cryoinjury: freezing caused coalescence of lipid droplets, loss of α-tubulin structure and apparent aggregation of F-actin. A combination of diapause and cold acclimation substantially lowered the temperature at which these morphological disruptions occurred. Larvae that recovered from a freezing challenge repaired F-actin aggregation but not lipid droplet coalescence or α-tubulin structure. Our observations indicate that lipid coalescence and damage to α-tubulin are non-lethal forms of freeze injury, and suggest that repair or removal (rather than protection) of actin proteins is a potential mechanism of acquired freeze tolerance.

• Klíčová slova
• Actin, Chymomyza, Cytoskeleton, Diapause, Drosophilid, Freeze tolerance,
• MeSH
• aklimatizace * MeSH
• cytoskelet fyziologie   MeSH
• Drosophilidae cytologie   růst a vývoj   fyziologie   MeSH
• gastrointestinální trakt cytologie   fyziologie   MeSH
• larva cytologie   růst a vývoj   fyziologie   MeSH
• malpighické trubice cytologie   fyziologie   MeSH
• nízká teplota škodlivé účinky   MeSH
• tukové těleso cytologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

This paper examines the impacts of extremely hot and cold days on mortality in Russia, using a 25-year regional panel data. Unlike other studies, the sequence of those extreme days is also taken into account, that is, the impacts of both single and consecutive (i.e. heat waves and cold spells) extreme days are estimated simultaneously. We demonstrate the importance of accounting for the sequence of extreme days. We also disentangle the impacts of those extremes by age and gender. The findings suggest that single hot days increase mortality, while single cold days do not affect mortality. On the other hand, both consecutive hot and consecutive cold days increase mortality in females and males for all age groups, although males are affected more severely. Overall, consecutive days with extreme temperatures impose considerable costs to society in terms of years of life lost. Thus, ignoring the sequences of extreme days that are likely to increase in the future because of climate change may have critical implications for mitigation policies.

• Klíčová slova
• Climate change, Cold spells, Extreme weather, Heat waves, Mortality, Russia,
• MeSH
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• mortalita trendy   MeSH
• nízká teplota škodlivé účinky   MeSH
• počasí * MeSH
• postup MeSH
• senioři MeSH
• sexuální faktory MeSH
• věkové faktory MeSH
• vysoká teplota škodlivé účinky   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Rusko epidemiologie   MeSH

The bean bug (Riptortus pedestris) is a pest of soybeans and other legumes in Japan and other Asian countries. It enters a facultative adult diapause on exposure to short days. While photoperiodism and diapause are well understood in R. pedestris, knowledge of cold tolerance is very limited, as is information on the effect of diapause on cold tolerance. We examined the effect of photoperiod, cold acclimation, and feeding status on cold tolerance in R. pedestris. We found that cold acclimation significantly increased survival at -10°C in both long- and short-day adult R. pedestris. Since the difference in cold survival between long- and short-day cold-acclimated groups was only marginal, we conclude that entering diapause is not crucial for R. pedestris to successfully pass through cold acclimation and become cold tolerant. We observed similar effects in 5th instar nymphs, with both long- and short-day cold-acclimated groups surviving longer cold exposures compared with non-acclimated groups. Starvation, which was tested only in adult bugs, had only a negligible and negative impact on cold survival. Although cold tolerance significantly increased with cold acclimation in adult bugs, supercooling capacity unexpectedly decreased. Our results suggest that changes in supercooling capacity as well as in water content are unrelated to cold tolerance in R. pedestris. An analysis of metabolites revealed differences between the treatments, and while several metabolites markedly increased with cold acclimation, their concentrations were too low to have a significant effect on cold tolerance.

• Klíčová slova
• Riptortus pedestris, cold acclimation, cold tolerance, diapause,
• MeSH
• aklimatizace * fyziologie   MeSH
• diapauza hmyzu fyziologie   MeSH
• Heteroptera metabolismus   fyziologie   MeSH
• metabolomika MeSH
• nízká teplota škodlivé účinky   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

This study extends our previous work by examining the effects of alpha2-adrenoceptors under cold stimulation involving the increase of myogenic vascular oscillations as increases of very-low-frequency and low-frequency of the blood pressure variability. Forty-eight adult male Sprague-Dawley rats were randomly divided into four groups: vehicle; yohimbine; hexamethonium+yohimbine; guanethidine+yohimbine. Systolic blood pressure, heart rate, power spectral analysis of spontaneous blood pressure and heart rate variability and spectral coherence at very-low-frequency (0.02 to 0.2 Hz), low-frequency (0.2 to 0.6 Hz), and high-frequency (0.6 to 3.0 Hz) regions were monitored using telemetry. Key findings are as follows: 1) Cooling-induced pressor response was attenuated by yohimbine and further attenuated by hexamethonium+yohimbine and guanethidine+yohimbine, 2) Cooling-induced tachycardia response of yohimbine was attenuated by hexamethonium+yohimbine and guanethidine+yohimbine, 3) Different patterns of power spectrum reaction and coherence value compared hexamethonium+yohimbine and guanethidine+yohimbine to yohimbine alone under cold stimulation. The results suggest that sympathetic activation of the postsynaptic alpha2-adrenoceptors causes vasoconstriction and heightening myogenic vascular oscillations, in turn, may increase blood flow to prevent tissue damage under stressful cooling challenge.

• MeSH
• alfa-2-adrenergní receptory - antagonisté farmakologie   MeSH
• alfa-2-adrenergní receptory fyziologie   MeSH
• hemodynamika účinky léků   fyziologie   MeSH
• krevní tlak účinky léků   fyziologie   MeSH
• krysa rodu Rattus MeSH
• náhodné rozdělení MeSH
• nízká teplota škodlivé účinky   MeSH
• potkani Sprague-Dawley MeSH
• srdeční frekvence účinky léků   fyziologie   MeSH
• telemetrie metody   MeSH
• vazokonstrikce účinky léků   fyziologie   MeSH
• yohimbin farmakologie   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• alfa-2-adrenergní receptory - antagonisté MeSH
• alfa-2-adrenergní receptory MeSH
• yohimbin MeSH

Activation of the hypothalamic-pituitary-adrenal (HPA) axis is important for maintenance of homeostasis during stress. Recent studies have shown a connection between the HPA axis and adipose tissue. The present study investigated the effect of acute heterotypic stress on plasma levels of adrenocorticotropic hormone (ACTH), corticosterone (CORT), leptin, and ghrelin in adult male rats with respect to neonatal maternal social and physical stressors. Thirty rat mothers and sixty of their male progeny were used. Pups were divided into three groups: unstressed control (C), stressed by maternal social stressor (S), stressed by maternal social and physical stressors (SW). Levels of hormones were measured in adult male progeny following an acute swimming stress (10 min) or no stress. ELISA immunoassay was used to measured hormones. The ACTH and CORT levels were significantly increased in all groups of adult progeny after acute stress; however, CORT levels were significantly lower in both neonatally stressed groups compared to controls. After acute stress, plasma leptin levels were decreased in the C and SW groups but increased in the S group. The data suggest that long-term neonatal stressors lead to lower sensitivity of ACTH receptors in the adrenal cortex, which could be a sign of stress adaptation in adulthood. Acute stress in adult male rats changes plasma levels of leptin differently relative to social or physical neonatal stressors.

• MeSH
• adrenokortikotropní hormon krev   MeSH
• biologické markery krev   MeSH
• fyziologický stres fyziologie   MeSH
• ghrelin krev   MeSH
• kortikosteron krev   MeSH
• krysa rodu Rattus MeSH
• leptin krev   MeSH
• matka - expozice noxám škodlivé účinky   MeSH
• nízká teplota škodlivé účinky   MeSH
• novorozená zvířata MeSH
• potkani Wistar MeSH
• psychický stres krev   etiologie   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• adrenokortikotropní hormon MeSH
• biologické markery MeSH
• ghrelin MeSH
• kortikosteron MeSH
• leptin MeSH