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10 záznamů v PubMed Filtry

Článek

Illuminating the cellular and molecular mechanism of the potential toxicity of methacrylate monomers used in biomaterials

Juráňová, Jana
Autor Juráňová, Jana ORCID Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacky University, Hněvotínská 3, Olomouc, Czech Republic Faculty of Medicine and Dentistry, Institute of Molecular and Translational Medicine, Palacky University, Hněvotínská 5, Olomouc, Czech Republic

Drug and chemical toxicology. 2020 May ; 43 (3) : 266-278. [epub] 20190104

Drug Chem Toxicol
ISSN 1525-6014 | 0148-0545
Zdroj

The cytotoxicity of methacrylate-based biopolymers crosslinked by in situ photopolymerization has been attributed mainly to residual methacrylate monomers released due to incomplete polymerization. The residual monomers, primarily triethyleneglycol dimethacrylate or 2-hydroxyethyl methacrylate, may irritate adjacent tissue, or be released into the bloodstream and reach practically all tissues. Increased production of reactive oxygen species, which may be connected to concomitant glutathione depletion, has been the most noticeable effect observed in vitro following the exposure of cells to methacrylates. Radical scavengers such as glutathione or N-acetylcysteine represent the most important cellular strategy against methacrylate-induced toxicity by direct adduct formation, resulting in monomer detoxification. Reactive oxygen species may participate in methacrylate-induced genotoxic or pro-apoptotic effects and cell-cycle arrest via induction of corresponding molecular pathways in cells. A deeper understanding of the biological mechanisms and effects of methacrylates widely used in various bioapplications may enable a better estimation of potential risks and thus, selection of a more appropriate composition of polymer material to eliminate potentially harmful substances such as triethyleneglycol dimethacrylate.

Klíčová slova
HEMA, ROS, TEGDMA, cytotoxicity, genotoxicity,
MeSH
acetylcystein farmakologie MeSH
biokompatibilní materiály chemie toxicita MeSH
glutathion metabolismus MeSH
kyseliny polymethakrylové chemie toxicita MeSH
lidé MeSH
methakryláty chemie toxicita MeSH
polyethylenglykoly chemie toxicita MeSH
reaktivní formy kyslíku metabolismus MeSH
scavengery volných radikálů farmakologie MeSH
zvířata MeSH
Check Tag
lidé MeSH
zvířata MeSH
Publikační typ
časopisecké články MeSH
přehledy MeSH
Názvy látek
acetylcystein MeSH
biokompatibilní materiály MeSH
glutathion MeSH
hydroxyethyl methacrylate MeSH Prohlížeč
kyseliny polymethakrylové MeSH
methakryláty MeSH
polyethylenglykoly MeSH
reaktivní formy kyslíku MeSH
scavengery volných radikálů MeSH
triethylene glycol dimethacrylate MeSH Prohlížeč

Článek

HPMA copolymer-bound doxorubicin induces immunogenic tumor cell death

Current medicinal chemistry. 2013 ; 20 (38) : 4815-26.

Curr Med Chem
ISSN 1875-533X | 0929-8673
Zdroj

Treatment of murine EL4 T cell lymphoma with N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer conjugates of doxorubicin (Dox) leads to complete tumor regression and to the development of therapy-dependent longlasting cancer resistance. This phenomenon occurs with two types of Dox conjugates tested, despite differences in the covalent linkage of Dox to the polymer carrier. Such a cancer resistance cannot fully express in conventional treatment with free Dox, due to substantial immunotoxicity of the treatment, which was not observed in the polymer conjugates. In this study, calreticulin (CRT) translocation and high mobility group box-1 protein (HMGB1) release was observed in EL4 cells treated with a conjugate releasing Dox by a pH-dependent manner. As a result, the treated tumor cells were engulfed by dendritic cells (DC) in vitro, and induced their expression of CD80, CD86, and MHC II maturation markers. Conjugates with Dox bound via an amide bond only increased translocation of HSPs to the membrane, which led to an elevated phagocytosis but was not sufficient to induce increase of the maturation markers on DCs in vitro. Both types of conjugates induced engulfment of the target tumor cells in vivo, that was more intense than that seen with free Dox. It means that the induction of anti-tumor immunity documented upon treatment of EL4 lymphoma with HPMA-bound Dox conjugates does not rely solely on CRT-mediated cell death, but involves multiple mechanisms.

Článek

Studium drázdivých látek extrahovaných z poly(HEMA) gelů
[Irritants extracted from poly(HEMA) gel]

Ceskoslovenska farmacie. 1988 Oct ; 37 (8) : 368-71.

Cesk Farm
ISSN 0009-0530
Zdroj

MeSH
dráždivé látky toxicita MeSH
inbrední kmeny potkanů MeSH
krysa rodu Rattus MeSH
kyseliny polymethakrylové toxicita MeSH
polyhydroxyethylmethakrylát toxicita MeSH
protézy a implantáty škodlivé účinky MeSH
zvířata MeSH
Check Tag
krysa rodu Rattus MeSH
mužské pohlaví MeSH
zvířata MeSH
Publikační typ
anglický abstrakt MeSH
časopisecké články MeSH
Názvy látek
dráždivé látky MeSH
kyseliny polymethakrylové MeSH
polyhydroxyethylmethakrylát MeSH

Článek

Irritation effects of residual products derived from p(HEMA) gels. II. Compounds extracted from hydrogels

Biomaterials. 1988 Jul ; 9 (4) : 372-5.

ISSN 0142-9612
Zdroj

Samples of poly(2-hydroxyethyl methacrylate) p(HEMA) hydrogels were prepared using three different polymerization initiators. The gels were washed in water under standard conditions. The extracts were then examined for intradermal irritation in rats using a radioactive indicator (113mIn). The irritation effects were dependent on the concentrations of the irritating substances and also on the gel type. Solid discs made of the gels, washed to varying degrees of purity, were also implanted into rats. Tissue irritation, as well as some other biological responses, were followed in situ using the radioindicator and common histological techniques. The irritation effects were very mild (even with the unextracted gel material). A possible explanation for the events taking place at the site of implantation is presented.

MeSH
biokompatibilní materiály * MeSH
dráždivé látky * MeSH
exsudáty a transsudáty analýza MeSH
fibrin analýza MeSH
inbrední kmeny potkanů MeSH
krevní proteiny metabolismus MeSH
krysa rodu Rattus MeSH
kůže účinky léků patologie MeSH
kyseliny polymethakrylové toxicita MeSH
polyhydroxyethylmethakrylát toxicita MeSH
protézy a implantáty MeSH
radioizotopy india MeSH
zánět MeSH
zvířata MeSH
Check Tag
krysa rodu Rattus MeSH
mužské pohlaví MeSH
zvířata MeSH
Publikační typ
časopisecké články MeSH
Názvy látek
biokompatibilní materiály * MeSH
dráždivé látky * MeSH
fibrin MeSH
krevní proteiny MeSH
kyseliny polymethakrylové MeSH
polyhydroxyethylmethakrylát MeSH
radioizotopy india MeSH

Článek

Hydrogels in endovascular embolization. IV. Effect of radiopaque spherical particles on the living tissue

Biomaterials. 1988 Jul ; 9 (4) : 367-71.

ISSN 0142-9612
Zdroj

In this study we report the results of toxicological, histological and haematological experiments on radiopaque spherical particles based on poly(2-hydroxyethyl methacrylate). These particles have been developed for endovascular occlusion of various organs. Radiopacity was attained by two independent methods: the chemical attachment of radiopaque substances to the hydrogel or the precipitation of radiopaque substances in the hydrogel network. The first method yields particles that appear to have uniformly-distributed contrast material, but in the particles prepared by the second procedure the contrast material is predominantly located on the surface. The visibility of such particles by X-rays makes possible controlled embolus introduction and inspection of the polymer for long periods after embolization. Radiopaque contrasting changes the morphology and reduces the porosity of the material but supports quick thrombus formation. Embolic material implanted in rabbits becomes surrounded by a thin fibrous capsule and undergoes partial organization. This and other results of medico-biological investigations have fully demonstrated the biocompatibility of radiopaque spherical emboli, which can now be used clinically.

MeSH
biokompatibilní materiály * MeSH
kontrastní látky MeSH
králíci MeSH
kůže patologie MeSH
kyseliny polymethakrylové toxicita MeSH
mikroskopie elektronová rastrovací MeSH
polyhydroxyethylmethakrylát toxicita MeSH
terapeutická embolizace * MeSH
zvířata MeSH
Check Tag
králíci MeSH
zvířata MeSH
Publikační typ
časopisecké články MeSH
Názvy látek
biokompatibilní materiály * MeSH
kontrastní látky MeSH
kyseliny polymethakrylové MeSH
polyhydroxyethylmethakrylát MeSH

Článek

Irritation effects of residual products derived from poly(2-hydroxyethyl methacrylate) gels. I. Testing of some model compounds

Biomaterials. 1988 May ; 9 (3) : 273-6.

ISSN 0142-9612
Zdroj

2-Hydroxyethyl methacrylate (HEMA) monomer and sodium benzoate, diluted with saline in the range 0-20%, were tested for intradermal irritation in rats. Radioactive indicator (113mIn) was used to quantify this biological response. At low concentrations (up to 1%) only a little irritation was recorded, while at higher levels (5% or more) a significant adverse reaction developed. The degree of irritation was dose dependent. In the concentration range 0-10%, the response was exponential. Model decomposition products derived from three different polymerization initiators were also tested. How the results obtained with the model irritants relate to real polymerization systems is discussed.