PURPOSE: Human colostrum and milk provide a newborn with immunomodulatory components, ensuring protection and proper development of the immune system. Secretory IgA antibodies in colostrum represent the first line of defence against harmful substances, but their potential spectra of reactivity with autoantigens remains unclear. Here, we characterised the repertoire of natural sectretory IgA autoantibodies in colostrum of healthy mothers. METHODS: The human colostrum samples from 39 healthy mothers were analyzed for autoantibodies by indirect immunofluorescence, dot blots, immunoblots and ELISA. RESULTS: We found that there is high diversity in reactivities of colostral IgA antibodies to autoantigens among individual samples. Using tissue sections and biochips commonly used for autoimmunity testing, we found that most samples reacted with monkey ovary (79.3%), monkey pancreatic tissue (78.6%), human HEp-2 cells (69%) and monkey adrenal gland (69.0%), fewer samples reacted with monkey liver tissue (47.2%), rat stomach (42.9%), monkey testicular tissue (41.4%), monkey salivary gland (39.3%), rat kidney (32.1%) and monkey cerebellar tissue (17.9%). At the protein level, we detected reactivity of IgA with 21 out of 25 (auto) antigens. The majority of the samples reacted with the pyruvate dehydrogenase complex, E3 ubiquitin ligase, cytosolic liver antigen, promyelocytic leukemia protein and nuclear pore glycoprotein-210. Using ELISA, we found reactivity of colostral IgA antibodies against examined extractable nuclear antigens, double stranded DNA, phospholipids and neutrophil cytoplasm. CONCLUSIONS: The broad spectrum of polyreactive natural autoantibodies present in human colostrum may contribute to proper development of mucosal immune system of the breastfed infant.

• MeSH
• autoantigeny imunologie   metabolismus   MeSH
• autoprotilátky imunologie   metabolismus   MeSH
• dospělí MeSH
• ELISA MeSH
• Haplorrhini MeSH
• imunoglobulin A sekreční biosyntéza   imunologie   MeSH
• imunohistochemie MeSH
• kojenec MeSH
• kojení MeSH
• kolostrum chemie   imunologie   MeSH
• krysa rodu Rattus MeSH
• laktace imunologie   MeSH
• lidé MeSH
• matky MeSH
• mladiství MeSH
• proteiny imunologie   metabolismus   MeSH
• specificita protilátek MeSH
• těhotenství MeSH
• vazba proteinů MeSH
• zvířata MeSH
• Check Tag
• dospělí MeSH
• kojenec MeSH
• krysa rodu Rattus MeSH
• lidé MeSH
• mladiství MeSH
• těhotenství MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• autoantigeny MeSH
• autoprotilátky MeSH
• imunoglobulin A sekreční MeSH
• proteiny MeSH

After oral administration of live oral vaccines COLINFANT and MUTAFLOR prepared from non-enteropathogenic E. coli strains, both strains colonized effectively the intestine in full-term and preterm infants and remained for many weeks showing, that they were capable to establish themselves as a resident strain in the infant's gut. The presence of E. coli stimulated significantly antibody production in gut, saliva and serum of colonized infants. An early induction of secretory IgA production is important particularly in formula-fed infants, where it partly replaces the lacking immunoglobulin supplied with mother milk. In full-term and premature infants the early presence of non-pathogenic E. coli strains in the intestine decreased significantly the presence of pathogenic bacterial strains in the intestine but also other mucosal surfaces of the body. The COLINFANT strain decreased the number of nosocomial infections, mortality rate in connection with infection, and the need for antibiotics. Both strains replaced successfully pathogenic strains in carriers after treatment with antibiotics.

• MeSH
• aplikace orální MeSH
• bakteriální vakcíny aplikace a dávkování   MeSH
• Escherichia coli klasifikace   růst a vývoj   imunologie   MeSH
• imunoglobulin A sekreční biosyntéza   MeSH
• imunoglobulin M biosyntéza   MeSH
• infekce spojené se zdravotní péčí imunologie   prevence a kontrola   MeSH
• infekce vyvolané Escherichia coli imunologie   prevence a kontrola   MeSH
• lidé MeSH
• nemoci nedonošenců imunologie   prevence a kontrola   MeSH
• novorozenec MeSH
• probiotika * terapeutické užití   MeSH
• protilátky bakteriální biosyntéza   MeSH
• střeva mikrobiologie   MeSH
• Check Tag
• lidé MeSH
• novorozenec MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH
• Názvy látek
• bakteriální vakcíny MeSH
• imunoglobulin A sekreční MeSH
• imunoglobulin M MeSH
• protilátky bakteriální MeSH

Antibodies to mumps virus were detected in 63.5% of saliva samples from mumps patients. The secretory antibody response was of primary type. Specific IgM antibodies were found in some samples collected early after the onset of disease. Specific IgA were detected in later obtained samples. Persons over 15 years of age reacted more often and more promptly than the children. The authors discuss the possible significance of prior antigenic stimulation by related paramyxoviruses (namely parainfluenzaviruses) for the intensity of local antibody response to mumps virus infection.

• MeSH
• dítě MeSH
• dospělí MeSH
• ELISA MeSH
• fluorescenční protilátková technika MeSH
• imunoglobulin A sekreční biosyntéza   MeSH
• imunoglobulin G biosyntéza   MeSH
• imunoglobulin M biosyntéza   MeSH
• lidé MeSH
• příušnice imunologie   MeSH
• protilátky virové biosyntéza   MeSH
• sliny imunologie   MeSH
• věkové faktory MeSH
• virus příušnic imunologie   MeSH
• Check Tag
• dítě MeSH
• dospělí MeSH
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• imunoglobulin A sekreční MeSH
• imunoglobulin G MeSH
• imunoglobulin M MeSH
• protilátky virové MeSH

• MeSH
• buněčná imunita MeSH
• dítě MeSH
• enteritida imunologie   MeSH
• imunoglobulin A sekreční biosyntéza   imunologie   MeSH
• infekce imunologie   MeSH
• lidé MeSH
• novorozenec MeSH
• sliznice imunologie   MeSH
• střevní sliznice imunologie   MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• novorozenec MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• imunoglobulin A sekreční MeSH