1. Selection strategies for broilers must balance rapid growth with the welfare and health of animals, strategies must deal with the trade-off with other vital functions.2. Divergent selection of Japanese quail for high (HG) and low (LG) relative body weight gain between 11 and 28 days of age has been conducted to accelerate linear phase growth without influencing the final adult body weight. Higher body growth rate is often connected with a weakened immune system. Therefore, the present study explored the immunological characterisation of quail from HG and LG lines, which differ substantially in their growth rate.3. The trial evaluated the maternal investment to immunologically active substances, cell-mediated immunity stimulated by phytohaemagglutinin (PHA) injection and the acute phase of the immune response to lipopolysaccharide (LPS) administration in three different phases of early postnatal growth.4. Except for higher lysozyme activity in the LG group when compared to the HG line, the maternal investment did not differ between the two lines. Plasma antibody concentrations responded quickly to any change in growth rate in both lines. Overall, it seems that initial rapid growth of the LG line had long-lasting effects on immune responsiveness, even after the growth rate of the HG line escalated during the linear phase of growth.5. The study indicated that changes in the growth rate caused by the selection for growth in meat-type Japanese quail can influence the acute phase of the immune response and development of the immune system.

• Klíčová slova
• Immunity, Japanese quail, LPS, PHA, selection,
• MeSH
• buněčná imunita MeSH
• bursa Fabricii anatomie a histologie   patologie   MeSH
• Coturnix růst a vývoj   imunologie   MeSH
• exprese genu MeSH
• fytohemaglutininy aplikace a dávkování   MeSH
• hmotnostní přírůstek imunologie   MeSH
• imunitní systém růst a vývoj   imunologie   MeSH
• imunoglobuliny analýza   MeSH
• interleukin-6 genetika   MeSH
• lipopolysacharidy aplikace a dávkování   MeSH
• muramidasa analýza   MeSH
• protilátky krev   MeSH
• slezina anatomie a histologie   patologie   MeSH
• vejce analýza   klasifikace   MeSH
• velikost orgánu MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• fytohemaglutininy MeSH
• IgY MeSH Prohlížeč
• imunoglobuliny MeSH
• interleukin-6 MeSH
• lipopolysacharidy MeSH
• muramidasa MeSH
• phytohemagglutinin-P MeSH Prohlížeč
• protilátky MeSH

Antioxidant, anti-inflammatory and anti-atherogenic effects have been associated with elevations of unconjugated bilirubin (UCB) in serum and with the induction of heme oxygenase-1 (HO-1), the rate-limiting enzyme in UCB synthesis. The aim of this study was to investigate the intracellular metabolism and antioxidant properties of UCB in human hepatoblastoma HepG2 cells and tissues of Wistar rats exposed to oxidative stressors and lipopolysaccharide (LPS), respectively. Intracellular UCB concentrations in HepG2 cells correlated with its levels in culture media (p < 0.001) and diminished lipid peroxidation in a dose-dependent manner (p < 0.001). Moreover, induction of HO-1 with sodium arsenite led to 2.4-fold (p = 0.01) accumulation of intracellular UCB over basal level while sodium azide-derived oxidative stress resulted in a 60% drop (p < 0.001). This decrease was ameliorated by UCB elevation in media or by simultaneous induction of HO-1. In addition, hyperbilirubinemia and liver HO-1 induction in LPS-treated rats resulted in a 2-fold accumulation of tissue UCB (p = 0.01) associated with enhanced protection against lipid peroxidation (p = 0.02). In conclusion, hyperbilirubinemia and HO-1 induction associated with inflammation and oxidative stress increase intracellular concentrations of UCB, thus enhancing the protection of cellular lipids against peroxidation. Therefore, the previously reported protective effects of hyperbilirubinemia and HO-1 induction are at least in part due to intracellular accumulation of UCB.

• MeSH
• aktivace enzymů účinky léků   MeSH
• antioxidancia metabolismus   MeSH
• arsenitany farmakologie   MeSH
• bilirubin aplikace a dávkování   analogy a deriváty   metabolismus   MeSH
• buňky Hep G2 MeSH
• hem analýza   MeSH
• hemoxygenasa-1 metabolismus   MeSH
• hyperbilirubinemie metabolismus   MeSH
• krysa rodu Rattus MeSH
• lidé MeSH
• lipopolysacharidy aplikace a dávkování   MeSH
• methemalbumin farmakologie   MeSH
• oxidační stres účinky léků   MeSH
• peroxidace lipidů účinky léků   MeSH
• potkani Wistar MeSH
• sloučeniny sodíku farmakologie   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antioxidancia MeSH
• arsenitany MeSH
• bilirubin MeSH
• hem MeSH
• hemoxygenasa-1 MeSH
• HMOX1 protein, human MeSH Prohlížeč
• lipopolysacharidy MeSH
• methemalbumin MeSH
• sloučeniny sodíku MeSH
• sodium arsenite MeSH Prohlížeč

A rat model of early sepsis induced by lipopolysaccharide (LPS) combined with interleukin-2 (IL-2) was developed. The primary aim was to assess the pharmacokinetics of gentamicin and sepsis-induced pathophysiological changes. Moreover, the effects on the glomerular filtration rate and tubular function were studied in septic and control rats. First, an intravenous (i.v.) bolus of LPSIL-2 (1 mg/kg-Pseudomonas aeruginosa, 15 µg/kg IL-2) or saline (controls, C) was administred. The Wistar rats were treated 30 min after LPSIL-2 with gentamicin as a 3 mg/kg i.v. bolus followed 10 min later by an i.v. 170-min infusion (GE, 0.09 mg/kg·min(-1)). The monitoring of vital functions, biochemistry and GE concentrations was performed. Creatinine clearance was 2-3 times lower and fractional urea excretion was 3-4 times less in septic rats as compared to controls(p<0.05), although urine flow was comparable. Capillary leakage caused a 55% elevation in the volume of distribution (V(c)) in the LPSIL+GE group vs. C+GE (p<0.05). The renal CL(ge) was less (2.2±0.59 vs. 3.8±0.53 mL/min·kg(-1), p<0.05), while the total CL(ge) was comparable (5.9±1.5 vs. 6.7±1.1 mL/min·kg(-1); p=0.30). In the LPSIL+GE group relative to C+GE, the half-life (t(1/2)) was 79% higher (p<0.05) and GE concentrations detected at the end of the study in the plasma and kidney were elevated 2.5-fold (p=0.09) and 2.2-fold (p<0.05), respectively. The model reproduced several consequences of early sepsis like in patients such as capillary leak, a decreased glomerular filtration rate (GFR) and the changes in pharmacokinetics of GE (increased values of V(c) and t(1/2) and a drop in renal CL(ge) proportional to that of CL(cr)). Nonrenal routes which, for the most part, compensate the reduced renal CL(ge) in septic rats deserve further study.

• MeSH
• antibakteriální látky krev   farmakokinetika   moč   MeSH
• gentamiciny krev   farmakokinetika   moč   MeSH
• hodnoty glomerulární filtrace účinky léků   MeSH
• interleukin-2 aplikace a dávkování   MeSH
• kapilární permeabilita účinky léků   MeSH
• krysa rodu Rattus MeSH
• ledviny účinky léků   metabolismus   patofyziologie   MeSH
• lipopolysacharidy aplikace a dávkování   MeSH
• modely nemocí na zvířatech * MeSH
• potkani Wistar MeSH
• sepse metabolismus   patofyziologie   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antibakteriální látky MeSH
• gentamiciny MeSH
• interleukin-2 MeSH
• lipopolysacharidy MeSH

BACKGROUND: Ankylosing enthesopathy (ANKENT) is an animal model of human ankylosing spondylitis. ANKENT is an inflammatory disease affecting the ankle and tarsal joints of the hind limbs in susceptible mouse strains. In the disease, the participation of intestinal microbiota components was suggested. Therefore, we attempted to increase the incidence of ANKENT by systemic administration of lipopolysaccharide (LPS), which is a component of bacterial cellular walls and stimulates inflammatory processes. METHODS: ANKENT occurrence, serum cytokine profiles, spleen cellular composition and in vitro cytokine response to LPS were analysed in LPS-treated and control LPS-untreated B10.BR male mice. RESULTS: Contrary to expectations, LPS treatment decreased the incidence of ANKENT in LPS-treated group compared to control LPS-untreated group. Flow cytometry analysis of splenocytes showed an increased percentage of macrophages, dendritic cells and neutrophils and a decreased percentage of B cells, T cells and T helper cells in LPS-treated males following LPS administration. In addition, LPS-treated males had significantly elevated IL-6 and IL-10 serum levels. At 20-22 weeks after the final LPS application, splenocytes from LPS-treated mice were more susceptible to in vitro LPS stimulation than those of the controls and produced significantly higher levels of TNFα and IL-6. CONCLUSIONS: Repeated systemic stimulation with microbial component lipopolysaccharide in early adulthood significantly reduced the incidence of ANKENT in B10.BR mice and this finding can support the "hygiene hypothesis". In LPS-treated mice, the innate immunity parameters and the level of anti-inflammatory IL-10 cytokine were significantly increased. Nevertheless, the immunological mechanism of the LPS protective effect remains unclear.

• MeSH
• ankylózující spondylitida krev   imunologie   prevence a kontrola   MeSH
• časové faktory MeSH
• injekce intraperitoneální MeSH
• interleukin-10 krev   MeSH
• interleukin-6 krev   MeSH
• kultivované buňky MeSH
• lipopolysacharidy aplikace a dávkování   farmakologie   MeSH
• lymfocyty účinky léků   imunologie   MeSH
• myši inbrední C57BL MeSH
• myši MeSH
• přirozená imunita účinky léků   MeSH
• průtoková cytometrie MeSH
• slezina účinky léků   imunologie   MeSH
• TNF-alfa metabolismus   MeSH
• upregulace MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• IL10 protein, mouse MeSH Prohlížeč
• interleukin-10 MeSH
• interleukin-6 MeSH
• lipopolysacharidy MeSH
• TNF-alfa MeSH

Peroxisome proliferator-activated receptor-gamma (PPAR- gamma), a member of the nuclear hormone receptor superfamily of ligand-activated transcription factors, possesses anti-inflammatory properties. The purpose of the present study was to investigate the profile of PPAR-gamma expression in the lung and to explore its functional significance in lipopolysaccharide (LPS)-induced acute lung injury. Thirty male Wistar rats were randomly assigned to one of the following five groups: saline control group and different LPS groups (2 h, 4 h, 6 h and 8 h after LPS 6 mg/kg i.v.). At predefined time points, blood samples were collected to measure plasma level of tumor necrosis factor (TNF)-alpha and lungs were removed to assay histopathological changes, wet-to-dry weight (W/D) ratio, myeloperoxidase (MPO) activity and TNF-alpha level. Expression of PPAR-gamma and activation of nuclear factor (NF)-kapaB p65 in lung tissues were also examined in each group. LPS injection resulted in marked lung damage and elevated levels of W/D ratio and MPO activity in the lung. Increased levels of TNF-alpha were also observed in the plasma and lung. These inflammatory events were associated with reduced expression of PPAR-gamma protein and with activation of NF-kapaB in the lung. Our data suggest that decreased expression of PPAR-gamma protein in lungs may contribute to the ongoing pulmonary inflammation and tissue injury in endotoxemia.

• MeSH
• exprese genu účinky léků   MeSH
• krysa rodu Rattus MeSH
• lipopolysacharidy aplikace a dávkování   toxicita   MeSH
• messenger RNA genetika   metabolismus   MeSH
• peroxidasa metabolismus   MeSH
• plíce účinky léků   metabolismus   patologie   MeSH
• plicní edém genetika   metabolismus   patologie   MeSH
• polymerázová řetězová reakce s reverzní transkripcí MeSH
• potkani Wistar MeSH
• PPAR gama genetika   metabolismus   MeSH
• syndrom dechové tísně chemicky indukované   genetika   metabolismus   MeSH
• TNF-alfa metabolismus   MeSH
• transkripční faktor RelA metabolismus   MeSH
• western blotting MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• lipopolysaccharide, E coli O55-B5 MeSH Prohlížeč
• lipopolysacharidy MeSH
• messenger RNA MeSH
• peroxidasa MeSH
• PPAR gama MeSH
• TNF-alfa MeSH
• transkripční faktor RelA MeSH

The aim of the study was to elucidate the effects of induced leukocyte migration into the bovine mammary gland on the manifestations of early and late apoptotic features of neutrophils cultivated in vitro. The Latin square design was used in two experiments, each involving four experimental repetitions in 4 clinically healthy virgin heifers. The neutrophil early apoptotic features were detected by flow cytometric detection (FCM) of phosphatidyl-serine translocation. Late neutrophil apoptotic features were detected by ELISA quantitation of histone-complexed DNA fragments. Leukocyte influx induction was accomplished by using four inducers: i) sterile buffered saline solution (PBS); ii) 5 % glucose solution (GLU); iii) synthetic muramyl dipeptide analogue (MDP); and iv) lipopolysaccharide (LPS), administered into the mammary gland lumen. Leukocytes from mammary glands were obtained by mammary gland lumen lavages after influx induction. The total cell counts in lavages increased after treatment by all inducers in comparison to the counts before influx induction (P<0.001). Cell counts were higher and differed significantly by MDP and LPS (P<0.01) in contrast to PBS. The highest proportion of neutrophils was induced by LPS (P<0.01). After three-hour incubation, light microscopy examination revealed the highest manifestation of neutrophil apoptosis after induction by GLU (P<0.05). The lowest apoptosis manifestation, though statistically non-significant, was detected after induction by MDP and LPS. Determination of early manifestation of neutrophil apoptosis revealed the lowest manifestation of neutrophil apoptosis after induction by LPS (P<0.01). The results of late manifestation of neutrophil apoptosis revealed the highest proportion of apoptotic neutrophils after induction by GLU (P<0.05). The manifestation of secondary necrosis of apoptotic neutrophils or neutrophil lysis after 3 h of incubation was low and not significant. In conclusion, certain inducers of neutrophil migration into the lumen of bovine mammary glands (GLU and LPS in the present experiments) significantly influence the manifestation of neutrophil apoptosis during their subsequent in vitro incubation.

• MeSH
• apoptóza účinky léků   fyziologie   MeSH
• glukosa aplikace a dávkování   MeSH
• kultivované buňky MeSH
• leukocyty účinky léků   fyziologie   MeSH
• lipopolysacharidy aplikace a dávkování   MeSH
• mléčné žlázy zvířat cytologie   účinky léků   fyziologie   MeSH
• neutrofily účinky léků   fyziologie   MeSH
• pohyb buněk účinky léků   fyziologie   MeSH
• skot MeSH
• zvířata MeSH
• Check Tag
• skot MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• glukosa MeSH
• lipopolysacharidy MeSH

Immunoprotective potential of delivered lipopolysaccharide (LPS) preparation from Klebsiella pneumoniae was determined in a murine model of lobar pneumonia. Protection was assessed with three doses of LPS (25, 50 and 100 microg; without any adjuvant) administered intranasally or intramuscularly. After evaluation of lung tissue (bacterial load and histopathology), no significant protection was observed at 25 microg with either application. A significant decrease in lung bacterial load coupled with fall in severity of lung lesions was observed with 50 microg (again both applications). At 100 microg dose, with intramuscular route, a further decrease in the lung bacterial load was shown compared to the 50 microg dose. In contrast, 100 microg LPS, when given intranasally, resulted in a higher bacterial colonization of the lung tissue and higher lung pathology; thus we recommend intramuscular instead of the intranasal route for developing protection against K. pneumoniae-mediated pneumonia with intact LPS-based vaccines.

• MeSH
• aplikace intranazální MeSH
• bakteriální pneumonie mikrobiologie   prevence a kontrola   MeSH
• imunizace * MeSH
• infekce bakteriemi rodu Klebsiella mikrobiologie   prevence a kontrola   MeSH
• injekce intramuskulární MeSH
• Klebsiella pneumoniae růst a vývoj   imunologie   MeSH
• lipopolysacharidy aplikace a dávkování   imunologie   izolace a purifikace   MeSH
• modely nemocí na zvířatech MeSH
• myši inbrední BALB C MeSH
• myši MeSH
• plíce mikrobiologie   patologie   MeSH
• počet mikrobiálních kolonií MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Indie MeSH
• Názvy látek
• lipopolysacharidy MeSH

Fever developing after intracerebral injections of lipopolysaccharide (LPS) to guinea-pigs were monophasic, with only one peak of inner body temperature slowly developing and longlasting in a dose range 20 to 200 ng of LPS. Latency time was inversely related to the dose of LPS. Indomethacin injected to the third brain ventricle did not abolish fever response.

• MeSH
• analýza rozptylu MeSH
• area praeoptica MeSH
• endotoxiny aplikace a dávkování   farmakologie   MeSH
• horečka chemicky indukované   patofyziologie   MeSH
• indomethacin farmakologie   MeSH
• injekce MeSH
• lipopolysacharidy aplikace a dávkování   MeSH
• morčata MeSH
• zvířata MeSH
• Check Tag
• morčata MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• endotoxiny MeSH
• indomethacin MeSH
• lipopolysacharidy MeSH

Natural and modified preparations of lipopolysaccharides and lipopolysaccharide-protein complexes isolated from the S- and R-form of Shigella dysenteriae serovar 1 were found to markedly inhibit the initial growth of mouse solid tumors derived from Németh-Kellner lymphoma, Gardner 6C3HED lymphoma, an ill-defined syngeneic lymphoma of DBA mice (Skalsky lymphoma) and LP-2 plasmacytoma. The biopreparations were given intraperitoneally, most frequently at a dose range from 50 to 200 micrograms per mouse; significant inhibitory effects on tumor growth were evidenced even in mice bearing tumors weighing 113 to 507 mg.

• MeSH
• bakteriální proteiny aplikace a dávkování   izolace a purifikace   terapeutické užití   MeSH
• hodnotící studie jako téma MeSH
• inbrední kmeny myší MeSH
• lipopolysacharidy aplikace a dávkování   izolace a purifikace   terapeutické užití   MeSH
• lymfom patologie   terapie   MeSH
• myši MeSH
• protinádorové látky aplikace a dávkování   izolace a purifikace   terapeutické užití   MeSH
• Shigella dysenteriae MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• bakteriální proteiny MeSH
• lipopolysacharidy MeSH
• protinádorové látky MeSH

Simultaneous expression of Fc receptors was studied on the model of peritoneal macrophages from two inbred strains of mice, A/J and C57BL/10ScSn, so-called high- and low-responders. Sheep red blood cells and/or synthetic polymer microspheres both coated with monoclonal antibodies of different isotypes were used for detection of Fc receptors. It was proved that the majority of macrophages bear more than one type of Fc receptors, moreover, macrophages expressing at least three types of Fc receptors simultaneously were detected. Both mouse strains vary in the presence of different subpopulations of macrophages bearing particular sets of Fc receptors.

• MeSH
• druhová specificita MeSH
• erytrocyty imunologie   MeSH
• imunizace MeSH
• imunoglobulin G biosyntéza   MeSH
• imunoglobulinové izotypy imunologie   MeSH
• inbrední kmeny myší MeSH
• kur domácí MeSH
• lipopolysacharidy aplikace a dávkování   imunologie   MeSH
• makrofágy imunologie   metabolismus   MeSH
• monoklonální protilátky imunologie   MeSH
• myši MeSH
• ovce MeSH
• peritoneální dutina cytologie   MeSH
• receptory Fc biosyntéza   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• imunoglobulin G MeSH
• imunoglobulinové izotypy MeSH
• lipopolysacharidy MeSH
• monoklonální protilátky MeSH
• receptory Fc MeSH