Prolactin-releasing peptide (PrRP) has been proposed to mediate the central satiating effects of cholecystokinin (CCK) through the vagal CCK1 receptor. PrRP acts as an endogenous ligand of G protein-coupled receptor 10 (GPR10), which is expressed at the highest levels in brain areas related to food intake regulation, e.g., the paraventricular hypothalamic nucleus (PVN) and nucleus of the solitary tract (NTS). The NTS and PVN are also significantly activated after peripheral CCK administration. The aim of this study was to determine whether the endogenous PrRP neuronal system in the brain is involved in the central anorexigenic effect of the peripherally administered CCK agonist JMV236 or the CCK1 antagonist devazepide and whether the CCK system is involved in the central anorexigenic effect of the peripherally applied lipidized PrRP analog palm-PrRP31 in fasted lean mice. The effect of devazepide and JMV236 on the anorexigenic effects of palm-PrRP31 as well as devazepide combined with JMV236 and palm-PrRP31 on food intake and Fos cell activation in the PVN and caudal NTS was examined. Our results suggest that the anorexigenic effect of JMV236 is accompanied by activation of PrRP neurons of the NTS in a CCK1 receptor-dependent manner. Moreover, while the anorexigenic effect of palm-PrRP31 was not affected by JMV236, it was partially attenuated by devazepide in fasted mice. The present findings indicate that the exogenously influenced CCK system may be involved in the central anorexigenic effect of peripherally applied palm-PrRP31, which possibly indicates some interaction between the CCK and PrRP neuronal systems.

• MeSH
• anorektika aplikace a dávkování   MeSH
• antagonisté hormonů aplikace a dávkování   MeSH
• chemokiny CC účinky léků   metabolismus   MeSH
• cholecystokinin metabolismus   MeSH
• devazepid aplikace a dávkování   MeSH
• hormon uvolňující prolaktin aplikace a dávkování   analogy a deriváty   MeSH
• injekce intraperitoneální MeSH
• injekce subkutánní MeSH
• myši inbrední C57BL MeSH
• myši MeSH
• nucleus paraventricularis hypothalami účinky léků   metabolismus   MeSH
• nucleus solitarius účinky léků   metabolismus   MeSH
• omezení příjmu potravy MeSH
• peptidové fragmenty aplikace a dávkování   MeSH
• přijímání potravy účinky léků   MeSH
• protoonkogenní proteiny c-fos metabolismus   MeSH
• signální transdukce MeSH
• sinkalid aplikace a dávkování   analogy a deriváty   MeSH
• stravovací zvyklosti účinky léků   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• anorektika MeSH
• antagonisté hormonů MeSH
• Ccl28 protein, mouse MeSH Prohlížeč
• chemokiny CC MeSH
• cholecystokinin MeSH
• devazepid MeSH
• Fos protein, mouse MeSH Prohlížeč
• hormon uvolňující prolaktin MeSH
• JMV 236 MeSH Prohlížeč
• palm11-PrRP31 MeSH Prohlížeč
• peptidové fragmenty MeSH
• protoonkogenní proteiny c-fos MeSH
• sinkalid MeSH

Generation of reactive oxygen species significantly contributes to the pathogenesis of acute renal failure (ARF) induced by myoglobin release. Ginsenosides (GS), the principal active ingredients of ginseng, is considered as an extremely good antioxidative composition of Chinese traditional and herbal drugs. The purpose of the present study was to investigate the protective effect of ginsenoside in rats with ARF on the changes of cholinergic nervous system in the kidney as well as on the involvement of mitogen-activated protein kinases (MAPK) in the hypothalamic paraventricular nuclei (PVN). In our assay, glycerol-induced acute renal failure in rats was employed to study the protective effects of ginsenoside. Our results indicated that the treatment of ARF rats with ginsenosides for 48 h significantly reduced lipid peroxidation, restored the superoxide dismutase (SOD) level. Meanwhile, the obvious increase of choline acetyltransferase-immunoreactivity (ChAT-IR) in the proximal convoluted tubular cells (PCT) was observed by immunohistochemistry in ARF+GS group. The same effect was also observed in the changes of p-ERK1/2-IR in the hypothalamic paraventricular nuclei. Our results suggest that ginsenoside administered orally may have a strong renal protective effect against glycerol-induced ARF, reduce the renal oxidative stress, and ginsenoside can also activate the cholinergic system in PCT, simultaneously MAPK signal pathway in the PVN was also activated.

• MeSH
• akutní poškození ledvin chemicky indukované   enzymologie   patologie   prevence a kontrola   MeSH
• antioxidancia aplikace a dávkování   farmakologie   MeSH
• aplikace orální MeSH
• časové faktory MeSH
• cholin-O-acetyltransferasa metabolismus   MeSH
• cytoprotekce MeSH
• fosforylace MeSH
• ginsenosidy aplikace a dávkování   farmakologie   MeSH
• glycerol MeSH
• malondialdehyd metabolismus   MeSH
• mitogenem aktivovaná proteinkinasa 1 metabolismus   MeSH
• mitogenem aktivovaná proteinkinasa 3 metabolismus   MeSH
• modely nemocí na zvířatech MeSH
• nucleus paraventricularis hypothalami účinky léků   enzymologie   MeSH
• oxidační stres účinky léků   MeSH
• peroxidace lipidů účinky léků   MeSH
• potkani Sprague-Dawley MeSH
• proximální tubuly ledvin účinky léků   enzymologie   patologie   MeSH
• signální transdukce účinky léků   MeSH
• superoxiddismutasa metabolismus   MeSH
• upregulace MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antioxidancia MeSH
• cholin-O-acetyltransferasa MeSH
• ginsenosidy MeSH
• glycerol MeSH
• malondialdehyd MeSH
• Mapk1 protein, rat MeSH Prohlížeč
• mitogenem aktivovaná proteinkinasa 1 MeSH
• mitogenem aktivovaná proteinkinasa 3 MeSH
• superoxiddismutasa MeSH

The hypothalamic supraoptic and paraventricular nuclei consist of oxytocin and arginine vasopressin synthesizing neurons that send projections to the neurohypophysis. A growing body of evidence in adult animals and young animals at near term confirmed the structure and function in the vasopressinergic and oxytocinergic network. However, whether those distinctive neural networks are formed before near term is largely unknown. This study determined the special patterns in location and distribution of oxytocin- and vasopressin-neurons in the paraventricular and supraoptic nuclei from preterm to term in the ovine fetuses. The results showed that oxytocin- and vasopressin-neurons were present in both nuclei at the three gestational time periods (preterm, near term, and term). In the paraventricular nuclei, vasopressin-cells concentrated mainly in the core of the middle magnocellular paraventricular nuclei, and oxytocin-cells were scattered surrounding the core. In the supraoptic nuclei, vasopressin-cells mostly located in the ventral part, and oxytocin-cells in the dorsal part. The data demonstrated that the special distributed patterns of vasopressin- and oxytocin-neuron network have formed in those two nuclei at least from preterm. Intracerebroventricular injection of angiotensin II significantly increased fetal plasma oxytocin and vasopressin levels at preterm, which was associated with an increase of oxytocin- and vasopressin-neuron activity marked with c-fos expression. The data provided new evidence for the structural and functional development of the oxytocin- and vasopressin-network before birth.

• MeSH
• angiotensin II aplikace a dávkování   MeSH
• arginin vasopresin krev   metabolismus   MeSH
• časové faktory MeSH
• gestační stáří MeSH
• injekce intraventrikulární MeSH
• nervová síť embryologie   metabolismus   MeSH
• neurony účinky léků   metabolismus   MeSH
• nucleus paraventricularis hypothalami účinky léků   embryologie   metabolismus   MeSH
• nucleus supraopticus účinky léků   embryologie   metabolismus   MeSH
• ovce MeSH
• oxytocin krev   metabolismus   MeSH
• protoonkogenní proteiny c-fos metabolismus   MeSH
• těhotenství MeSH
• zvířata MeSH
• Check Tag
• těhotenství MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• angiotensin II MeSH
• arginin vasopresin MeSH
• oxytocin MeSH
• protoonkogenní proteiny c-fos MeSH

BACKGROUND: CART (cocaine- and amphetamine-regulated transcript) peptide and cholecystokinin (CCK) are neuromodulators involved in feeding behavior. This study is based on previously found synergistic effect of leptin and CCK on food intake and our hypothesis on a co-operation of the CART peptide and CCK in food intake regulation and Fos activation in their common targets, the nucleus tractus solitarii of the brainstem (NTS), the paraventricular nucleus (PVN), and the dorsomedial nucleus (DMH) of the hypothalamus. RESULTS: In fasted C57BL/6 mice, the anorexigenic effect of CART(61-102) in the doses of 0.1 or 0.5 microg/mouse was significantly enhanced by low doses of CCK-8 of 0.4 or 4 microg/kg, while 1 mg/kg dose of CCK-A receptor antagonist devazepide blocked the effect of CART(61-102) on food intake. After simultaneous administration of 0.1 microg/mouse CART(61-102) and of 4 microg/kg of CCK-8, the number of Fos-positive neurons in NTS, PVN, and DMH was significantly higher than after administration of each particular peptide. Besides, CART(61-102) and CCK-8 showed an additive effect on inhibition of the locomotor activity of mice in an open field test. CONCLUSION: The synergistic and long-lasting effect of the CART peptide and CCK on food intake and their additive effect on Fos immunoreactivity in their common targets suggest a co-operative action of CART peptide and CCK which could be related to synergistic effect of leptin on CCK satiety.

• MeSH
• antagonisté hormonů farmakologie   MeSH
• benzodiazepinony farmakologie   MeSH
• devazepid farmakologie   MeSH
• fenylmočovinové sloučeniny farmakologie   MeSH
• hubenost * MeSH
• injekce intraperitoneální MeSH
• injekce intraventrikulární MeSH
• myši inbrední C57BL MeSH
• myši MeSH
• neurony účinky léků   metabolismus   MeSH
• nucleus dorsomedialis hypothalami účinky léků   fyziologie   MeSH
• nucleus paraventricularis hypothalami účinky léků   fyziologie   MeSH
• nucleus solitarius účinky léků   fyziologie   MeSH
• pátrací chování účinky léků   fyziologie   MeSH
• peptidové fragmenty farmakologie   MeSH
• proteiny nervové tkáně farmakologie   MeSH
• protoonkogenní proteiny c-fos metabolismus   MeSH
• receptor cholecystokininu A antagonisté a inhibitory   MeSH
• receptor cholecystokininu B antagonisté a inhibitory   MeSH
• regulace chuti k jídlu účinky léků   fyziologie   MeSH
• sinkalid farmakologie   MeSH
• synergismus léků MeSH
• vztah mezi dávkou a účinkem léčiva MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antagonisté hormonů MeSH
• benzodiazepinony MeSH
• Cocaine- and Amphetamine-Regulated Transcript Protein MeSH
• devazepid MeSH
• fenylmočovinové sloučeniny MeSH
• L 365260 MeSH Prohlížeč
• peptidové fragmenty MeSH
• proteiny nervové tkáně MeSH
• protoonkogenní proteiny c-fos MeSH
• receptor cholecystokininu A MeSH
• receptor cholecystokininu B MeSH
• sinkalid MeSH

The thyrotropin-releasing hormone (TRH) is a molecule with widespread distribution through many organ systems. The function of TRH is probably not identical in each system so that TRH synthesis and secretion may be unique for each system under specific experimental conditions. The present study was designed to explore the common and diverse features of the regulation of TRH encoded with the same gene in two different organs: hypophysiotropic hypothalamus and pancreatic islets. During in vitro incubation, the TRH content in hypothalamic structures remained stable while that in isolated pancreatic islets increased sharply. In contrast to the pancreatic islets, exposure to different concentrations of D-glucose did not affect TRH release from the hypothalamic paraventricular nucleus or median eminence. This divergence in the regulation of the hypophysiotropic and pancreatic TRH systems may be related to differences in the role of TRH produced in these tissues.

• MeSH
• chlorid draselný farmakologie   MeSH
• eminentia medialis účinky léků   metabolismus   MeSH
• glukosa farmakologie   MeSH
• hormon uvolňující thyreotropin metabolismus   MeSH
• hypothalamus účinky léků   metabolismus   MeSH
• krysa rodu Rattus MeSH
• kultivované buňky MeSH
• Langerhansovy ostrůvky účinky léků   metabolismus   MeSH
• nucleus paraventricularis hypothalami účinky léků   metabolismus   MeSH
• potkani Wistar MeSH
• vápník farmakologie   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• chlorid draselný MeSH
• glukosa MeSH
• hormon uvolňující thyreotropin MeSH
• vápník MeSH

Caryometric analysis was used for the study of changes in the cell nucleus volume of the neurons of nucleus paraventricularis, nucleus arcuatus, and ependyma of the third cerebral ventricle of sheep after the administration of Gn-RH, followed by exposure to X-rays. The test animals were 12 ewes in physiological anoestrus and two rams. The trials were conducted in spring. The first group of four sheep and two rams were left as controls; in the ewes of the second group the hypothalamo-hypophysial region was irradiated by exposure to 516.5 mC/kg (200 R); in the four ewes of the third group, ovaries were directly irradiated at laparotomy by exposure to 64.4 mC/kg (250 R). The ewes of the second and third group were treated with an i. m. administration of 400 micrograms Gn-RH per head before irradiation. The excisions were collected and processed the tenth day from irradiation. The studied material was fixed by injection of 10% formalin and finished by another dose of 10% formalin after the excision of the brains. The paraffin slices were stained with haematoxylin-eosine. The caryometric analysis was performed at 3000-fold magnification, 200 cells being measured in each sample. Changes in neurosecretory cells were described in the regions of nucleus paraventricularis, nucleus arcuatus and in the ependyma of the third cerebral ventricle. The results of the caryometric analysis of nucleus paraventricularis and nucleus arcuatus suggest that the administration of Gn-RH and irradiation of the hypothalamo-hypophysial region, and direct irradiation of ovaries, stimulate the studied cerebral structures. The changes observed in the ependyma of the third cerebral ventricle after the administration of Gn-RH and subsequent irradiation of the hypothalamo-hypophysial region are insignificant; it is only after the direct irradiation of ovaries that these cells are inhibited by an indirect effect through the feedback mechanism.

• MeSH
• buněčné jádro účinky záření   MeSH
• ependym účinky léků   účinky záření   ultrastruktura   MeSH
• hormony uvolňující hypofyzární hormony farmakologie   MeSH
• karyometrie MeSH
• nucleus arcuatus hypothalami účinky léků   účinky záření   ultrastruktura   MeSH
• nucleus paraventricularis hypothalami účinky léků   účinky záření   ultrastruktura   MeSH
• ovce anatomie a histologie   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• Názvy látek
• hormony uvolňující hypofyzární hormony MeSH