Moderately halophilic bacteria utilizing phenol as the sole carbon source were isolated by selective enrichment from sea water. The isolate (Gram-negative motile rods) was identified as Deleya venusta. It grew well in the presence of up to 1600 mg/L of phenol and 8% NaCl under aerobic conditions. When the cells were treated with chloramphenicol prior to the addition of phenol they did not utilize added phenol, even after prolonged incubation. Thus, the enzymes necessary for phenol metabolism appeared to be inducible.

• MeSH
• Arthrobacter classification   growth & development   isolation & purification   MeSH
• Bacteria classification   growth & development   isolation & purification   MeSH
• Bacteriological Techniques MeSH
• Biodegradation, Environmental MeSH
• Water Pollutants, Chemical metabolism   MeSH
• Sodium Chloride MeSH
• Phenol metabolism   MeSH
• Halomonas classification   growth & development   isolation & purification   MeSH
• Culture Media MeSH
• Seawater microbiology   MeSH
• Vibrio classification   growth & development   isolation & purification   MeSH
• Publication type
• Journal Article MeSH
• Evaluation Study MeSH
• Names of Substances
• Water Pollutants, Chemical MeSH
• Sodium Chloride MeSH
• Phenol MeSH
• Culture Media MeSH

Spread-plating belongs to traditional microbiological methods employed for quantification of subsurface microflora during bioremediation projects in the Czechia. Concentration of degrading organisms is estimated from the number of colonies grown on agar plates supplied with contaminant as the sole carbon source. The data obtained during in situ bioremediation of the Dacice site contaminated by cutting oil suggests that changes in the composition of the carbon source in the subsurface may cause a discrepancy between laboratory data and situation in subsurface.

• MeSH
• Bacteria isolation & purification   metabolism   MeSH
• Bacteriological Techniques * MeSH
• Biodegradation, Environmental MeSH
• Culture Media MeSH
• Environmental Microbiology * MeSH
• Oils metabolism   MeSH
• Carbon MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Culture Media MeSH
• Oils MeSH
• Carbon MeSH