Zygosaccharomyces rouxii is a hemiascomycetous yeast known for its high osmotolerance, the basis of which still remains unknown. By exploring the Génolevures I database, four Z. rouxii fragments homologous to Saccharomyces cerevisiae centromeres were identified. Two of them were subjected to further analysis. Their function as centromeres in Z. rouxii was proved, and they were localized to Z. rouxii chromosomes II and VII, respectively. The species-specificity of centromeres was observed; plasmids with a Z. rouxii centromere were not recognized as centromeric in S. cerevisiae, and a S. cerevisiae centromere did not function as a centromere in Z. rouxii. Constructed plasmids bearing Z. rouxii centromeres serve as the first specific centromeric plasmids, and thus contribute to the so-far limited set of genetic tools needed to study the Z. rouxii specific features.

• MeSH
• centromera genetika   MeSH
• DNA fungální genetika   MeSH
• DNA primery genetika   MeSH
• druhová specificita MeSH
• genetické vektory MeSH
• plazmidy genetika   MeSH
• Saccharomyces cerevisiae genetika   MeSH
• sekvence nukleotidů MeSH
• transformace genetická MeSH
• Zygosaccharomyces genetika   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Názvy látek
• DNA fungální MeSH
• DNA primery MeSH

The osmotolerant yeast Pichia sorbitophila was found to differ from other yeast species, not only from the conventional ones (Saccharomyces cerevisiae, Schizosaccharomyces pombe), but also from those widely known as osmotolerant (Debaryomyces hansenii, Zygosaccharomyces rouxii). P. sorbitophila was able to survive extremely high extracellular concentrations of salts (e.g., saturated solution of KCl) and other osmolytes (70% glucitol), although it is not classified as halophilic (or osmophilic). P. sorbitophila assimilated a broad range of carbon and nitrogen sources with extreme effectiveness. On solid media, P. sorbitophila created colonies of variable shapes and sizes in relation to media composition, number of colonies on the plate and cultivation conditions. Colonies were able to produce long-distance signals between each other that resulted in growth inhibition of the facing parts of both colonies, but were not inhibited by colonies of other yeast species growing on the same plate. Though sometimes P. sorbitophila has been indicated as a synonym of P. farinosa, comparative physiological studies together with PCR amplification of P. farinosa DNA fragments homologous to known P. sorbitophila genes provided a strong indication that this strain should be classified as a separate species.

• MeSH
• chloridy farmakologie   MeSH
• DNA fungální analýza   MeSH
• dusík metabolismus   MeSH
• kultivační média MeSH
• osmolární koncentrace MeSH
• osmotický tlak MeSH
• Pichia klasifikace   genetika   růst a vývoj   fyziologie   MeSH
• polymerázová řetězová reakce MeSH
• Saccharomyces cerevisiae růst a vývoj   fyziologie   MeSH
• Schizosaccharomyces růst a vývoj   fyziologie   MeSH
• uhlík metabolismus   MeSH
• Zygosaccharomyces růst a vývoj   fyziologie   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Názvy látek
• chloridy MeSH
• DNA fungální MeSH
• dusík MeSH
• kultivační média MeSH
• uhlík MeSH