Preventive vaccination by a hsp90-expressing DNA vaccine and recombinant hsp90 protein vaccine, both derived from the Candida albicans hsp90 using BALB-c mouse model of systemic candidiasis, was performed. Hsp90 mRNA was cloned from a clinical isolate of C. albicans, converted to cDNA and cloned into vaccination plasmid pVAX1. Two methods of DNA application were tested: intramuscular (i.m.) and intradermal (i.d.) injection. Recombinant protein was applied by i.d. injection with Freund's adjuvant; the control groups received PBS or Freund's adjuvant only. Mice were vaccinated and after 19 d re-vaccinated. After 3 weeks, the mice were challenged with the live C. albicans in a dose of 5 x 10(6) CFU per mouse. After the challenge, the mice vaccinated i.d. with DNA vaccine survived for 39 and 64% longer compared to those receiving Freund's adjuvant and/or PBS, respectively. The i.m. application of the DNA vaccine did not provide any significant protectivity. The serum level of anti-candida-hsp90 serum IgG antibodies correlated with the survival rate in both i.d. protein and DNA vaccination approaches. We stressed the importance of specific humoral immunity in the mouse model of systemic candidiasis.

• MeSH
• Survival Analysis MeSH
• Candida albicans immunology   MeSH
• Vaccines, DNA administration & dosage   immunology   MeSH
• Freund's Adjuvant MeSH
• Fungal Vaccines administration & dosage   immunology   MeSH
• Immunoglobulin G blood   MeSH
• Injections, Intradermal MeSH
• Injections, Intramuscular MeSH
• Candidiasis prevention & control   MeSH
• Disease Models, Animal MeSH
• Mice, Inbred BALB C MeSH
• Mice MeSH
• HSP90 Heat-Shock Proteins administration & dosage   genetics   immunology   MeSH
• Antibodies, Fungal blood   MeSH
• Vaccines, Subunit administration & dosage   immunology   MeSH
• Vaccines, Synthetic administration & dosage   immunology   MeSH
• Animals MeSH
• Check Tag
• Mice MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Comparative Study MeSH
• Names of Substances
• Vaccines, DNA MeSH
• Freund's Adjuvant MeSH
• Fungal Vaccines MeSH
• Immunoglobulin G MeSH
• HSP90 Heat-Shock Proteins MeSH
• Antibodies, Fungal MeSH
• Vaccines, Subunit MeSH
• Vaccines, Synthetic MeSH

The levels of anti-Candida antibodies were determined in experimental animals immunized with 4 different yeast doses (0.3, 0.6, 1.2, 2.4 x 10(8) CFU) at weekly intervals. After immunization (sampling intervals 1, 2, and 3 months), the intravenous blood was examined for the presence of serum anti-Candida antibodies. After one month, the titers of anti-Candida antibodies reached 1:40-1: 1280 and remained at the same level after two months in the majority of animals; only in a few of them the titers increased or were detected de novo. After three months, when the animals were no longer immunized, a decreasing trend in antibody titers was detected in the majority of animals.

• MeSH
• Agglutination Tests MeSH
• Antigens, Fungal immunology   MeSH
• Candida albicans immunology   MeSH
• Immunization MeSH
• Rats MeSH
• Rats, Sprague-Dawley MeSH
• Antibodies, Fungal blood   MeSH
• Animals MeSH
• Check Tag
• Rats MeSH
• Female MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Antigens, Fungal MeSH
• Antibodies, Fungal MeSH

An indirect immunofluorescence assay was performed to detect antibodies to Candida albicans blastospores and germ tubes. Serum specimens were obtained from 82 patients with neoplastic diseases in the orofacial region and thrush of the oral mucosa. C. albicans was identified in the oral cavity of 63 patients investigated but serum anti-Candida antibodies were detected in only 23 of them. Serological examination showed that titers of antibodies to C. albicans blastospores ranged from 1:20 to 1:1280. High titers from 1:640 to 1:1280 were detected in patients without antibiotic, cytostatic, or radiotherapeutic treatment. The titers of antibodies to C. albicans germ tubes ranged from 1:20 to 1:640. Our results indicate that titers of antibodies to the C. albicans germ tubes were lower and were detected in a smaller number of patients.

• MeSH
• Candida albicans immunology   isolation & purification   MeSH
• Fluorescent Antibody Technique, Indirect MeSH
• Candidiasis complications   immunology   microbiology   MeSH
• Carcinoma blood   complications   microbiology   MeSH
• Humans MeSH
• Mouth Neoplasms blood   complications   microbiology   MeSH
• Candidiasis, Oral complications   immunology   microbiology   MeSH
• Antibodies, Fungal blood   MeSH
• Mouth Mucosa microbiology   MeSH
• Check Tag
• Humans MeSH
• Male MeSH
• Female MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Antibodies, Fungal MeSH