Patients in various clinical states of diabetes mellitus (according to the recommendation of the American Diabetes Association) as a primary diagnosis were examined for fungal infections by Candida species. Candida spp. were detected in urine, in the material taken from the mouth cavity, nails, skin lesions, ears and eyes, by cultivation on the Sabouraud agar, CHROMagar Candida, and by saccharide assimilation. In the group of diabetics with symptoms of oral candidiasis and denture stomatitis C. albicans was identified in 8 cases, C. tropicalis in 3, C. parapsilosis in 2; 1 strain of C. guilliermondii was also isolated. In patients with urinary tract infections the presence of C. albicans was shown in 12 cases; C. parapsilosis was detected in 6 cases and two strains of each C. tropicalis and C. krusei were also isolated. In patients with leg ulcers C. albicans (25 cases), C. parapsilosis (5), C. tropicalis (3) and one strain of each C. krusei and C. robusta were isolated. Otomycosis was associated with one strain of C. albicans, C. parapsilosis, C. tropicalis and C. guilliermondii. C. albicans was most frequently associated with onychomycosis, paronychia and endophthalmitis; C. parapsilosis was the second most rated yeast.

• MeSH
• Candida klasifikace   izolace a purifikace   MeSH
• diabetes mellitus MeSH
• kandidóza kožní komplikace   mikrobiologie   patologie   MeSH
• kandidóza komplikace   diagnóza   mikrobiologie   patologie   MeSH
• komplikace diabetu mikrobiologie   patologie   MeSH
• lidé MeSH
• orální kandidóza komplikace   mikrobiologie   patologie   MeSH
• retrospektivní studie MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

Production of secreted aspartate proteinases was determined in a set of 646 isolates of Candida and non-Candida yeast species collected from 465 patients of the University Hospital in Olomouc (Czechia) in the period 1995-2002, and Candida samples obtained from 64 healthy volunteers using solid media developed for this purpose. Using random amplified polymorphic DNA analysis (RAPD) 79 Candida isolates from blood were analyzed to show potential relationships between clustering of the fingerprints and extracellular proteolytic activity of these strains. C. albicans, C. tropicalis and C. parapsilosis possess always proteolytic activity while non-Candida species did not display any proteolysis. A tight relationship between fingerprints and extracellular proteolysis in the Candida isolates was not shown. A remarkable consistency between fingerprint clusters and proteolysis occurred in a subset of C. parapsilosis samples. Suboptimal pH of the growth medium was shown to facilitate the investigation of potential co-incidence of genotypic and phenotypic traits.

• MeSH
• aspartátové endopeptidasy fyziologie   MeSH
• Candida enzymologie   patogenita   MeSH
• faktory virulence fyziologie   MeSH
• fungální proteiny fyziologie   MeSH
• koncentrace vodíkových iontů MeSH
• lidé MeSH
• technika náhodné amplifikace polymorfní DNA MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• aspartátové endopeptidasy MeSH
• faktory virulence MeSH
• fungální proteiny MeSH

A total of 43 patients, 11 males and 32 females, with paronychia of the fingernails were examined for the presence of Candida spp. The yeast species isolated were identified using standard laboratory methods, including germ-tube production, morphology on rice agar with Tween 80, and mainly fermentation and assimilation of saccharides. In the male group, two Candida species were detected: C. albicans as the dominant species in 9 patients and C. parapsilosis in 2 cases. Similarly, C. albicans was the prevalent species also in females (n = 17); other Candida species detected were C. parapsilosis (n = 7), C. tropicalis (5) and C. krusei (3). In addition to the genus Candida, the following anaerobic and aerobic microorganisms were isolated from patients of both groups: Fusobacterium spp., Bacteroides spp., Staphylococcus aureus, alpha-hemolytic streptococci, group A beta-hemolytic streptococci, Klebsiella pneumoniae, Neisseria spp. and Pseudomonas aeruginosa.

• MeSH
• Candida klasifikace   izolace a purifikace   patogenita   MeSH
• dospělí MeSH
• druhová specificita MeSH
• kandidóza etiologie   mikrobiologie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• mykologické určovací techniky MeSH
• mykologie metody   MeSH
• nehty mikrobiologie   MeSH
• paronychie etiologie   mikrobiologie   MeSH
• rizikové faktory MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

The collection wild-type strain of Candida albicans was used to obtain auxotrophic and colony-morphology mutants by 'nitrosoguanidine' treatment. Intraspecific protoplast fusion induced by Ca(2+)-poly(ethyleneglycol) was carried out in various pairings between the auxotrophic strain producing smooth colonies and containing blastospores and the colony-morphology mutants containing a mixture of blastospores and pseudohyphae or only hyphae. Hybrids exhibiting full or partial complementation were obtained when mutants producing smooth colonies and colony-morphology variants of different origins were fused. The mutation responsible for the colony-morphology character (if various types of colony morphomutants were crossed) proved to be recessive or semidominant. Representative hybrids exhibited elevated DNA contents as measured by flow cytometry. To illustrate various cell types, and especially the intermediate one (never observed in natural isolates), a preparation method was further developed for scanning electron microscopic studies.

• MeSH
• Candida albicans genetika   růst a vývoj   MeSH
• DNA fungální genetika   MeSH
• křížení genetické MeSH
• kultivační média MeSH
• methylnitronitrosoguanidin farmakologie   MeSH
• mikroskopie elektronová rastrovací MeSH
• mutace * MeSH
• polyethylenglykoly farmakologie   MeSH
• průtoková cytometrie MeSH
• testy genetické komplementace MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• DNA fungální MeSH
• kultivační média MeSH
• methylnitronitrosoguanidin MeSH
• polyethylenglykoly MeSH

Restriction fragment length polymorphism analysis of the 5.8S rRNA gene and the internal transcribed spacers (ITS1 and ITS2) was used for examination of 66 isolates belonging to 19 species. Intraspecies variability was found in the examined region of 11 species (Candida albicans, C. catenulata, C. colliculosa, C. glabrata, C. kefyr, C. melinii, C. parapsilosis, C. guillermondii, C. solanii, C. tropicalis, Saccharomyces cerevisiae). Region of ITS-5.8S rDNA was amplified using the primers ITS1 and ITS4. The amplicons were digested by HaeIII, HinfI and CfoI. The recognized intraspecies variability was confirmed in the second step, in which the shorter fragments of this region were amplified using primers ITS1 and ITS2 and analyzed by capillary electrophoresis. Considerable intraspecific variability renders this method unsuitable for species identification, whereas it can be useful for epidemiological tracing of isolates.

• MeSH
• Candida klasifikace   genetika   MeSH
• DNA fungální analýza   MeSH
• DNA primery * MeSH
• druhová specificita MeSH
• elektroforéza kapilární MeSH
• genetická variace * MeSH
• geny rRNA MeSH
• houby klasifikace   genetika   MeSH
• lidé MeSH
• mezerníky ribozomální DNA analýza   genetika   MeSH
• mykologické určovací techniky MeSH
• mykózy mikrobiologie   MeSH
• polymerázová řetězová reakce metody   MeSH
• RNA ribozomální 5.8S analýza   genetika   MeSH
• Saccharomyces cerevisiae klasifikace   genetika   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• hodnotící studie MeSH
• práce podpořená grantem MeSH
• Názvy látek
• DNA fungální MeSH
• DNA primery * MeSH
• mezerníky ribozomální DNA MeSH
• RNA ribozomální 5.8S MeSH