The impact of postantibiotic effect (PAE) of carbapenems (imipenem, meropenem) on the metabolism (biosynthesis of macromolecules, respiration), cell-surface hydrophobicity and motility of a clinical isolate of Enterobacter cloacae was examined. The metabolism was evaluated after 16 h and after 1 d of cultivation using 2x and 4x minimum inhibitory concentrations (MIC) of both antibiotics for the induction of PAE. Imipenem at 4 x MIC did not induce PAE. After a 16-h cultivation (in the postantibiotic phase of both carbapenems), inhibition of nucleosynthesis and protein synthesis was found; after a 1-d cultivation, during regrowth stimulation of mainly 14C-leucine incorporation was found. The presence of the exogenous intermediates of citrate cycle, viz. 2-oxoglutarate, increased the respiratory activity of the cells. The cell-surface hydrophobicity (evaluated by three methods--bacterial adhesion to hydrocarbon, nitrocellulose-filter test and salt-aggregation test) decreased after PAE of both carbapenems; meropenem was more effective. Motility (an important virulence factor) was inhibited in the postantibiotic phase of both carbapenems; the 4 x MIC caused a higher inhibition.

• MeSH
• časové faktory MeSH
• Enterobacter cloacae chemie   účinky léků   fyziologie   MeSH
• hydrofobní a hydrofilní interakce MeSH
• imipenem farmakologie   MeSH
• karbapenemy farmakologie   MeSH
• lidé MeSH
• meropenem MeSH
• pohyb účinky léků   MeSH
• thienamyciny farmakologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH
• Názvy látek
• imipenem MeSH
• karbapenemy MeSH
• meropenem MeSH
• thienamyciny MeSH

The postantibiotic effect (PAE) and the postantibiotic sub-MIC effect (PASME) of ciprofloxacin, pefloxacin and amikacin were studied for Salmonella typhimurium and S. enteritidis strains. PAE was induced by 2 x and 4 x MIC of antibiotics studied for 0.5 h. After PAE and PASME their effect on prophage induction of a lysogenic S. typhimurium strain and on Congo red binding for both strains as a marker of their surface hydrophobicity was examined. The longest PAE was found after treatment with ciprofloxacin, higher values being observed with S. typhimurium. PAEs of pefloxacin and amikacin were much lower, except for the suprainhibitory concentration 4 x MIC of amikacin with S. enteritidis (6.9h). PASMEs of ciprofloxacin did not allow any regrowth of either strain. For other antibiotics the PASMEs were different while concentrations of 2 x MIC + 0.2 x MIC and 0.3 x MIC, and of 4 x MIC + 0.1 x MIC, 0.2 x MIC and 0.3 x MIC of amikacin did not allow any regrowth of S. enteritidis. PAEs of the antibiotics tested did not affect the Congo red binding by both Salmonella strains, but the PAEs of ciprofloxacin and pefloxacin expressively induced a prophage of lysogenic S. typhimurium strain. We noted the influence of Congo red binding after applying 4 x MIC + 0.1 x MIC, 0.2 x MIC and 0.3 x MIC of amikacin for S. typhimurium and 2 x MIC + 0.1 x MIC for S. enteritidis.

• MeSH
• aktivace viru účinky léků   MeSH
• amikacin aplikace a dávkování   farmakologie   MeSH
• antibakteriální látky aplikace a dávkování   farmakologie   MeSH
• antiinfekční látky aplikace a dávkování   farmakologie   MeSH
• bakteriofágy MeSH
• barvicí látky MeSH
• buněčná stěna chemie   účinky léků   MeSH
• chemické jevy MeSH
• ciprofloxacin aplikace a dávkování   farmakologie   MeSH
• fágy salmonel účinky léků   růst a vývoj   MeSH
• fyzikální chemie MeSH
• Kongo červeň MeSH
• mikrobiální testy citlivosti MeSH
• pefloxacin aplikace a dávkování   farmakologie   MeSH
• Salmonella enteritidis účinky léků   metabolismus   MeSH
• Salmonella typhimurium účinky léků   metabolismus   virologie   MeSH
• vztah mezi dávkou a účinkem léčiva MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH
• Názvy látek
• amikacin MeSH
• antibakteriální látky MeSH
• antiinfekční látky MeSH
• barvicí látky MeSH
• ciprofloxacin MeSH
• Kongo červeň MeSH
• pefloxacin MeSH

Aminoglycosides at 2x or 4x minimum inhibitory concentration induced postantibiotic effects against Pseudomonas aeruginosa lasting 3.5-4.9 h (gentamicin) and 0.5-3.7 h (selemycin). Postantibiotic effects of subinhibitory concentrations of the aminoglycosides tested were substantially longer. Some combinations of supra- and subinhibitory concentrations of antibiotics did not even allow any regrowth of the bacterial strain. The postantibiotic effects and postantibiotic effects of subinhibitory concentrations of gentamicin and selemycin were associated with changes of P. aeruginosa elastase and proteinase. Combinations of supra- and subinhibitory concentrations more pronouncedly suppressed enzymic activities than did suprainhibitory concentrations alone.

• MeSH
• antibakteriální látky farmakologie   MeSH
• endopeptidasy účinky léků   metabolismus   MeSH
• gentamiciny farmakologie   MeSH
• mikrobiální testy citlivosti MeSH
• pankreatická elastasa účinky léků   metabolismus   MeSH
• Pseudomonas aeruginosa účinky léků   enzymologie   metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antibakteriální látky MeSH
• endopeptidasy MeSH
• gentamiciny MeSH
• pankreatická elastasa MeSH