We investigated the possibility of utilizing both oleaginous yeast species accumulating large amounts of lipids (Yarrowia lipolytica, Rhodotorula glutinis, Trichosporon cutaneum, Candida sp.) and traditional biotechnological non-oleaginous ones characterized by high biomass yield (Kluyveromyces polysporus, Torulaspora delbrueckii, Saccharomyces cerevisiae) as potential producers of biofuel-utilizable and nutritionally valuable lipids. The main objective was to increase lipid accumulation by increasing C/P ratio together with higher C/N ratio, while maintaining high biomass yield. The C/N ratio of 30 was found to lead to higher biomass content and the total lipid content increased significantly with higher C/P ratio. With higher ratios of both C/N and C/P, the content of monounsaturated fatty acids (FAs) in cell lipids increased while polyunsaturated FAs decreased. Oleaginous yeast species had a lower proportion of unsaturated FAs (approx. 80 %) than non-oleaginous strains (approx. 90 %). At a C/N ratio of 30 and C/P ratio 1043, T. cutaneum produced a high amount of ω-6 unsaturated linoleic acid, the precursor of some prostaglandins, leukotrienes, and thromboxanes, while Candida sp. and K. polysporus accumulated a high content of palmitoleic acid.

• MeSH
• cytosol chemie   MeSH
• dusík metabolismus   MeSH
• fosfáty metabolismus   MeSH
• kultivační média chemie   MeSH
• kvasinky růst a vývoj   metabolismus   MeSH
• mastné kyseliny analýza   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• dusík MeSH
• fosfáty MeSH
• kultivační média MeSH
• mastné kyseliny MeSH

The growth of microorganisms is affected by cultivation conditions, concentration of carbon and nitrogen sources and the presence of trace elements. One of the new possibilities of influencing the production of cell mass or lipids is the use of lanthanides. Lanthanides are biologically non-essential elements with wide applications in technology and industry and their concentration as environmental contaminants is therefore increasing. Although non-essential, lanthanides have been proposed (and even used) to produce beneficial effects in plants but their mechanisms of action are unclear. Recently, it was suggested that they may replace essential elements or operate as potent blockers of Ca(2+) channels. We tested the effect of low concentrations of lanthanides on traditional biotechnologically useful yeast species (Kluyveromyces polysporus, Saccharomyces cerevisiae, Torulospora delbrueckii), and species capable of high accumulation of lipids (Rhodotorula glutinis, Trichosporon cutaneum, Candida sp., Yarrowia lipolytica). Low concentrations of lanthanum and monazite were conducive to an increase in cell mass and lipids and also higher production of palmitoleic acid, commonly used in cosmetics and medicine, and ω6-linoleic acid which is a precursor of thromboxanes, prostaglandins and leucotrienes.

• Klíčová slova
• Fatty acids, Lanthanides, Microbial lipids, Non-oleaginous yeasts, Oleaginous yeasts,
• MeSH
• biomasa MeSH
• kultivační média chemie   MeSH
• kvasinky účinky léků   růst a vývoj   MeSH
• lanthanoidy farmakologie   MeSH
• mastné kyseliny biosyntéza   MeSH
• metabolismus lipidů účinky léků   MeSH
• průmyslová mikrobiologie MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• kultivační média MeSH
• lanthanoidy MeSH
• mastné kyseliny MeSH