Sixty-five samples of cerebrospinal fluid (CSF) were evaluated using an automated cytoflow method with the CD-Sapphire hematology analyzer in order to investigate possible relationships between cell population patterns and diagnostic groups and better understand the biology of neurological disease. A basic panel of CD markers, including CD3/4/8/19/138/HLA-DR, was used to analyze CSF samples from clinical and laboratory confirmed cases of multiple sclerosis, neuroborreliosis, viral and bacterial neuroinfective diseases, malignant infiltrations of meninges and scavenger macrophagic reactions of the central nervous system. The principles of immune response and the contribution of cytological 'disease-related patterns' for these nosological entities are described. The distinct patterns of lymphocyte subpopulations in neuroborreliosis appear to be characteristic and could possibly serve as diagnostic indicators. Further verification and research will be necessary to clarify the significance and nature of CD4+ CD8+ positive subset in cerebrospinal fluid.

• MeSH
• CD antigeny mozkomíšní mok   imunologie   MeSH
• encefalitida virová imunologie   MeSH
• lidé MeSH
• lymská neuroborelióza imunologie   MeSH
• meningitida virová imunologie   MeSH
• monoklonální protilátky imunologie   MeSH
• mozkomíšní mok cytologie   imunologie   MeSH
• nemoci centrálního nervového systému mozkomíšní mok   diagnóza   imunologie   MeSH
• podskupiny lymfocytů cytologie   imunologie   MeSH
• průtoková cytometrie metody   MeSH
• roztroušená skleróza imunologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• hodnotící studie MeSH
• Názvy látek
• CD antigeny MeSH
• monoklonální protilátky MeSH

Selected cerebrospinal-fluid (CSF) parameters (intrathecal synthesis of Borrelia-specific antibodies, oligoclonal IgG bands, CSF-to-serum quotient of albumin as a marker of blood-CSF barrier function and cytology) and typical CSF profile in neuroborreliosis were evaluated with the aim of elucidating possible clinical and laboratory similarities of neuroborreliosis (NB) and other neurological diseases (OND). From the cohort of 58 patients (38 diagnosed for NB, 20 with OND) NB patients had positive Borrelia-specific IgG antibodies in 97 % and positive Borrelia-specific IgM antibodies in 55 %; oligoclonal IgG bands were detected in 55%. The blood-CSF barrier was impaired in 89%, positive cytology was detected in 97% of the NB patients. Evaluation of specific intrathecal synthesis improves CSF diagnosis of NB, therefore, a combined CSF analysis has to be considered along with the clinical picture and medical history when formulating the diagnosis of NB.

• MeSH
• Borrelia burgdorferi komplex * imunologie   MeSH
• diferenciální diagnóza MeSH
• hematoencefalická bariéra MeSH
• histocytochemie MeSH
• imunoglobulin G mozkomíšní mok   MeSH
• imunoglobulin M mozkomíšní mok   MeSH
• lidé MeSH
• lymská neuroborelióza mozkomíšní mok   diagnóza   MeSH
• mozkomíšní mok * chemie   cytologie   imunologie   MeSH
• nemoci nervového systému mozkomíšní mok   diagnóza   MeSH
• protilátky bakteriální mozkomíšní mok   MeSH
• sérový albumin mozkomíšní mok   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• imunoglobulin G MeSH
• imunoglobulin M MeSH
• protilátky bakteriální MeSH
• sérový albumin MeSH

In a group of 10,156 patients with neurological diseases, the IgM level was assessed (using laser nephelometry) both in cerebrospinal fluid (CSF) and serum; concentration of other 17 protein fractions was also simultaneously determined: albumin, immunoglobulins, acute phase reactants, complement components, apolipoproteins, proteinase inhibitors and alpha1-microglobulin. Total protein, element counts and glucose level were also evaluated. In patients with normal CSF findings, only limited statistically significant correlations were demonstrated between IgM and other CSF protein fractions while, in the group of patients with pathological CSF findings, significant correlations were found between CSF(IgM) and other immunoglobulins, complement fractions and the rate of intrathecal synthesis of immunoglobulins. Correlations were also found between CSF(IgM) and CSF antithrombin-III and alpha1-microglobulin. Correlations between CSF(IgM) and CSF apolipoproteins support the theory of CNS tissue destruction whenever the concentration of CSF apolipoproteins is elevated. Our data substantially contribute to establishing the diagnosis in patients with neurological diseases; simultaneous measurement of a high number of CSF proteins is becoming inevitable for a reasonable assessment of the CSF Protein Status.

• MeSH
• biologické markery MeSH
• imunoglobulin M krev   mozkomíšní mok   MeSH
• lidé MeSH
• lineární modely MeSH
• mozkomíšní mok chemie   cytologie   imunologie   MeSH
• nemoci nervového systému krev   mozkomíšní mok   imunologie   MeSH
• prediktivní hodnota testů MeSH
• proteiny v mozkomíšním moku chemie   MeSH
• zánět MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• biologické markery MeSH
• imunoglobulin M MeSH
• proteiny v mozkomíšním moku MeSH

A simple assay by polymerase chain reaction was used for the of detection of Borrelia burgdorferi, causative agent of Lyme borreliosis (LB). It involves no DNA purification and is based on the amplification of a specific region of ospA gene of B. burgdorferi, followed by direct detection of the PCR product with SYBR Green I by agarose gel electrophoresis. The method was used to analyze samples from patients with LB diagnosis, with presumable infection with the LB spirochete, those with unclear clinical symptoms and after the course of an antibiotic treatment. Spirochetal DNA was detected by PCR even in contaminated samples in which B. burgdorferi was overgrown by fungi and other bacteria. Spirochetal DNA was detected and borrelia species was identified in cerebrospinal fluid of two patients hospitalized with the diagnosis "fever of unknown origin". Western blot and ELISA were negative in both cases. Total analysis of 94 samples from the hospital in Ceské Budejovice (South Bohemia, Czechia) showed infection with B. burgdorferi sensu stricto in 11% and B. garinii in 15% of cases. The highest prevalence was found for B. afzelii (43%). Co-infection was confirmed in 24 % of the analyzed symplex; 7% of samples that were B. burgdorferi sensu lato positive gave no results in DNA amplification with B. burgdorferi sensu stricto-, B. garinii- and B. afzelii-specific primers. The proposed reliable, rapid, unexpensive and specific technique could form the basis of laboratory tests for routine detection and identification of Lyme-disease spirochete in different samples.

• MeSH
• antigeny povrchové genetika   MeSH
• bakteriální vakcíny MeSH
• benzothiazoly MeSH
• Borrelia burgdorferi komplex klasifikace   genetika   imunologie   izolace a purifikace   MeSH
• chinoliny MeSH
• diaminy MeSH
• DNA bakterií analýza   izolace a purifikace   MeSH
• elektroforéza v agarovém gelu MeSH
• ELISA MeSH
• genotyp MeSH
• imunoglobulin G krev   MeSH
• imunoglobulin M krev   MeSH
• lidé MeSH
• lipoproteiny genetika   MeSH
• lymeská nemoc diagnóza   MeSH
• mozkomíšní mok mikrobiologie   MeSH
• organické látky metabolismus   MeSH
• polymerázová řetězová reakce metody   MeSH
• proteiny vnější bakteriální membrány genetika   MeSH
• protilátky bakteriální krev   MeSH
• senzitivita a specificita MeSH
• sérum mikrobiologie   MeSH
• techniky typizace bakterií MeSH
• western blotting MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antigeny povrchové MeSH
• bakteriální vakcíny MeSH
• benzothiazoly MeSH
• chinoliny MeSH
• diaminy MeSH
• DNA bakterií MeSH
• imunoglobulin G MeSH
• imunoglobulin M MeSH
• lipoproteiny MeSH
• organické látky MeSH
• OspA protein MeSH Prohlížeč
• proteiny vnější bakteriální membrány MeSH
• protilátky bakteriální MeSH
• SYBR Green I MeSH Prohlížeč

Immunoelectrophoresis and its modifications were applied to analysis of a lipopolysaccharide-like component (LPS-LC) extracted from Borrelia garinii strains K24 and K48 isolated from Ixodes ricinus and Borrelia burgdorferi sensu stricto strain B31. A modification of the hot phenol-water method was used for isolation of LPS. Immunoelectrophoresis (IE) and crossed immunoelectrophoresis (CIE) of LPS-LC with polyclonal rabbit antisera revealed a pattern and properties partially similar to LPS from other Gram-negative bacteria. B. garinii LPS-LC formed in CIE a diffuse band extending from the start to the anode. Similarly, the shape and position of the band in IE did not show major differences from LPS of other Gram-negative bacteria. The LPS-LC extracted from the three genomic groups of B. burgdorferi sensu lato were found to have similar immunochemical properties irrespective of their genotype origin.

• MeSH
• Borrelia burgdorferi komplex chemie   genetika   imunologie   MeSH
• Borrelia burgdorferi chemie   imunologie   MeSH
• Borrelia chemie   imunologie   MeSH
• DNA bakterií genetika   MeSH
• druhová specificita MeSH
• imunoelektroforéza dvourozměrná MeSH
• imunoelektroforéza MeSH
• králíci MeSH
• lipopolysacharidy analýza   imunologie   MeSH
• protilátky bakteriální MeSH
• sekvence nukleotidů MeSH
• zvířata MeSH
• Check Tag
• králíci MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH
• Názvy látek
• DNA bakterií MeSH
• lipopolysacharidy MeSH
• protilátky bakteriální MeSH