The aim of this study was to analyze the functional properties of newly obtained films based on sodium alginate and lecithin with the addition of antioxidant-rich coffee extracts and to verify their potential as safe edible food packaging materials. In our study, we developed alginate-lecithin films enriched with green or roasted coffee bean extracts. The roasting process of coffee beans had a significant impact on the total phenolic content (TPC) in the studied extracts. The highest value of TPC (2697.2 mg GAE/dm3), as well as antioxidant activity (AA) (17.6 mM T/dm3), was observed for the extract of light-roasted coffee beans. Films with the addition of medium-roasted coffee extracts and baseline films had the highest tensile strength (21.21 ± 0.73 N). The addition of coffee extract improved the barrier properties of the films against UV light with a decrease in the transmittance values (200-400 nm), regardless of the type of extract added. Studies on Caco-2, HepG2 and BJ cells showed that digestated films were non-cytotoxic materials (100-0.1 μg/cm3) and had no negative effect on cell viability; an increase was noted for all cell lines, the highest after 48 h in a dose of 1 μg/cm3 for a film with medium-roasted coffee (194.43 ± 38.30) for Caco-2. The tested films at 20% digestate concentrations demonstrated the ability to reduce nitric oxide (NO) production in the RAW264.7 cell line by 25 to 60% compared to the control. Each of the tested films with coffee extracts had growth inhibitory properties towards selected species of bacteria.

• Klíčová slova
• antimicrobial activity, antioxidant activity, biopolymer-based packaging, coffee extracts, cytotoxicity, edible packaging, lecithin, nitric oxide, sodium alginate,
• MeSH
• algináty * chemie   farmakologie   MeSH
• antiflogistika * farmakologie   chemie   MeSH
• antiinfekční látky farmakologie   chemie   MeSH
• antioxidancia * farmakologie   chemie   MeSH
• buňky Hep G2 MeSH
• Caco-2 buňky MeSH
• Coffea chemie   MeSH
• jedlé filmy MeSH
• káva chemie   MeSH
• lecitiny * chemie   MeSH
• lidé MeSH
• myši MeSH
• obaly potravin metody   MeSH
• rostlinné extrakty * chemie   farmakologie   MeSH
• semena rostlinná chemie   MeSH
• viabilita buněk účinky léků   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• algináty * MeSH
• antiflogistika * MeSH
• antiinfekční látky MeSH
• antioxidancia * MeSH
• káva MeSH
• lecitiny * MeSH
• rostlinné extrakty * MeSH