The level of lysozyme in fat body, hemocytes and cell-free hemolymph from Galleria mellonella larvae infected with Pseudomonas aeruginosa was determined and evaluated. In the samples of fat body and hemocytes, an increase in lysozyme content was detected 1 d after infection and then a significant decrease was observed after a prolonged infection time. In the case of cell-free hemolymph, an increase in the lysozyme level was noticeable during the first 30 h post injection and stayed at a similar level for 42 h. The smaller decrease of the lysozyme level after 42 h might be associated with the development of bacteremia of P. aeruginosa in insects. In addition, the gradual increase in the content of lysozyme correlated with the increase of its activity in the hemolymph of the infected larvae as a response to injection with P. aeruginosa. The G. mellonella lysozyme appeared to be insensitive to extracellular proteinases produced in vivo by P. aeruginosa.

• MeSH
• hemocyty enzymologie   MeSH
• hemolymfa enzymologie   MeSH
• hmyzí proteiny metabolismus   MeSH
• larva enzymologie   mikrobiologie   MeSH
• muramidasa metabolismus   MeSH
• můry enzymologie   mikrobiologie   MeSH
• Pseudomonas aeruginosa fyziologie   MeSH
• tukové těleso enzymologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• hmyzí proteiny MeSH
• muramidasa MeSH

The relatedness between clinical isolates of P. aeruginosa obtained from patients during their stay in a Portuguese Central Hospital was evaluated. Genotypic fingerprinting (M13-PCR), phenotypic methods (biotyping and antibiotyping) and epidemiological information (spatial and temporal links) were used to evaluate the relatedness between 88 clinical isolates (68 patients), selected randomly out of 189. Sixty-two M13 types were found, 12 of them containing isolates from more than one patient. Thirty-four antibiotypes were found, as well as a significant association (p < 0.05) between epidemic isolates and multiresistance patterns. The nosocomial transmission of P. aeruginosa strains may be limited since M13 typing demonstrated a high degree of diversity among all the isolates, suggesting the occurrence of mainly independent infectious episodes. The results show the possible occurrence of cross-acquisition, cross-colonization and cross-infection and suggest an epidemic population structure for P. aeruginosa in this hospital.

• MeSH
• antibiotická rezistence MeSH
• DNA bakterií analýza   MeSH
• genotyp MeSH
• infekce spojené se zdravotní péčí epidemiologie   mikrobiologie   přenos   MeSH
• lidé MeSH
• nemocnice fakultní statistika a číselné údaje   MeSH
• polymerázová řetězová reakce MeSH
• pseudomonádové infekce epidemiologie   mikrobiologie   přenos   MeSH
• Pseudomonas aeruginosa genetika   izolace a purifikace   MeSH
• pulzní gelová elektroforéza MeSH
• vzorkové studie MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Portugalsko epidemiologie   MeSH
• Názvy látek
• DNA bakterií MeSH

The bla(PER-1) presence was sought by PCR in 289 ceftazidime resistant Gram-negative bacteria isolated at Dokuz Eylul University Hospital (Turkey) between 1998 and 2003. PER-1 production rates were 32.3, 33.9, 14.9 and 37.9% in the 1998-2000 period, 2001, 2002 and 2003, respectively. bla(PER-1) was detected in 46.2 and 35.9% of ceftazidime-resistant Pseudomonas aeruginosa and Acinetobacter baumannii isolates, respectively. ERIC-PCR results revealed that dissemination of two endemic clones for both P. aeruginosa (X and Y) and A. baumannii (A and B) was responsible for the high prevalence. Results of the conjugation tests and plasmid curing experiments suggested that bla(PER-1) was located on the chromosome in the representative strains. It was also shown for the first time that bla(PER-1) in a clinical isolate was associated with class-1 integron which could facilitate dissemination of bla(PER-1) among bacteria.

• MeSH
• Acinetobacter baumannii účinky léků   enzymologie   genetika   izolace a purifikace   MeSH
• antibakteriální látky farmakologie   MeSH
• beta-laktamasy genetika   MeSH
• beta-laktamová rezistence účinky léků   genetika   MeSH
• ceftazidim farmakologie   MeSH
• DNA fingerprinting MeSH
• infekce bakteriemi rodu Acinetobacter epidemiologie   přenos   MeSH
• integrony MeSH
• lidé MeSH
• mikrobiální testy citlivosti MeSH
• multigenová rodina MeSH
• nemocnice univerzitní MeSH
• polymerázová řetězová reakce metody   MeSH
• přenos genů horizontální MeSH
• pseudomonádové infekce epidemiologie   přenos   MeSH
• Pseudomonas aeruginosa účinky léků   enzymologie   genetika   izolace a purifikace   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Turecko epidemiologie   MeSH
• Názvy látek
• antibakteriální látky MeSH
• beta-lactamase PER-1 MeSH Prohlížeč
• beta-laktamasy MeSH
• ceftazidim MeSH

The influence of subinhibitory concentrations (1/2, 1/4, 1/8, 1/16 and 1/32 MIC) of amikacin and ciprofloxacin on the morphology and adherence of uropathogenic strains was studied. Intensity of morphological changes was proportional to the concentrations of these antibiotics. Morphological changes were the most prominent after bacterial exposure to sub-MICs of ciprofloxacin. These concentrations, especially 1/2 MIC of ciprofloxacin, induced the formation of filaments of E. coli, K. pneumoniae, K. oxytoca, E. cloacae and A. calcoaceticus biotype anitratus. No morphological changes were observed in P. aeruginosa, S. epidermidis and S. aureus cells after exposure to subinhibitory concentrations of both antibiotics. Sub-MICs of amikacin affected the changes in cell shape only slightly. The exposure of bacterial strains to 1/2 MIC of ciprofloxacin induced increased vacuolation of the cells. We observed shrinkage of the protoplasm and the pleated cell walls in comparison with control cells. The greatest loss of adherence ability occurred at 1/2 MIC of ciprofloxacin after a 1-d incubation.

• MeSH
• amikacin farmakologie   MeSH
• antiinfekční látky farmakologie   MeSH
• bakteriální adheze účinky léků   MeSH
• ciprofloxacin farmakologie   MeSH
• Enterobacteriaceae účinky léků   růst a vývoj   ultrastruktura   MeSH
• enterobakteriální infekce mikrobiologie   moč   MeSH
• epitelové buňky MeSH
• infekce močového ústrojí mikrobiologie   moč   MeSH
• lidé MeSH
• mikrobiální testy citlivosti MeSH
• mikroskopie elektronová rastrovací MeSH
• mikroskopie fázově kontrastní MeSH
• transmisní elektronová mikroskopie MeSH
• Check Tag
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• amikacin MeSH
• antiinfekční látky MeSH
• ciprofloxacin MeSH