Photodynamic inactivation (PDI) is a promising approach for the efficient killing of pathogenic microbes. In this study, the photodynamic effect of sulfonated polystyrene nanoparticles with encapsulated hydrophobic 5,10,15,20-tetraphenylporphyrin (TPP-NP) photosensitizers on Gram-positive (including multi-resistant) and Gram-negative bacterial strains was investigated. The cell viability was determined by the colony forming unit method. The results showed no dark cytotoxicity but high phototoxicity within the tested conditions. Gram-positive bacteria were more sensitive to TPP-NPs than Gram-negative bacteria. Atomic force microscopy was used to detect changes in the morphological properties of bacteria before and after the PDI treatment.

• MeSH
• Bacteria účinky léků   účinky záření   MeSH
• fotochemické procesy * MeSH
• fotochemoterapie metody   MeSH
• mikroskopie atomárních sil MeSH
• nanočástice * chemie   MeSH
• polystyreny * chemie   MeSH
• porfyriny aplikace a dávkování   chemie   MeSH
• příprava léků * MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• polystyreny * MeSH
• porfyriny MeSH
• tetraphenylporphyrin MeSH Prohlížeč

Novel biomaterials based on hydrophilic polycaprolactone and polyurethane (Tecophilic®) nanofibers with an encapsulated 5,10,5,20-tetraphenylporphyrin photosensitizer were prepared by electrospinning. The doped nanofiber textiles efficiently photo-generate O(2)((1)Δ(g)), which oxidize external chemical and biological substrates/targets. Strong photo-virucidal effects toward non-enveloped polyomaviruses and enveloped baculoviruses were observed on the surface of these textiles. The photo-virucidal effect was confirmed by a decrease in virus infectivity. In contrast, no virucidal effect was detected in the absence of light and/or the encapsulated photosensitizer.

• MeSH
• anthraceny chemie   MeSH
• antivirové látky farmakologie   MeSH
• Baculoviridae účinky léků   MeSH
• fotosenzibilizující látky farmakologie   MeSH
• inaktivace viru účinky léků   MeSH
• kapsida chemie   MeSH
• myši MeSH
• nanovlákna chemie   ultrastruktura   MeSH
• oxidace-redukce MeSH
• polyestery chemie   MeSH
• Polyomavirus účinky léků   MeSH
• polyurethany chemie   MeSH
• porfyriny farmakologie   MeSH
• rekombinace genetická genetika   MeSH
• singletový kyslík metabolismus   MeSH
• textilie * MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• anthraceny MeSH
• antivirové látky MeSH
• fotosenzibilizující látky MeSH
• polycaprolactone MeSH Prohlížeč
• polyestery MeSH
• polyurethany MeSH
• porfyriny MeSH
• singletový kyslík MeSH
• tetraphenylporphyrin MeSH Prohlížeč