Epigallocatechin-3-gallate (EGCG) is the main compound of green tea with well-described antioxidant, anti-inflammatory, and tumor-suppressing properties. However, EGCG at high doses was reported to cause liver injury. In this study, we evaluated the effect of EGCG on primary culture of rat hepatocytes and on rat liver mitochondria in permeabilized hepatocytes. The 24-hour incubation with EGCG in concentrations of 10 μmol/L and higher led to signs of cellular injury and to a decrease in hepatocyte functions. The effect of EGCG on the formation of reactive oxygen species (ROS) was biphasic. While low doses of EGCG decreased ROS production, the highest tested dose induced a significant increase in ROS formation. Furthermore, we observed a decline in mitochondrial membrane potential in cells exposed to EGCG when compared to control cells. In permeabilized hepatocytes, EGCG caused damage of the outer mitochondrial membrane and an uncoupling of oxidative phosphorylation. EGCG in concentrations lower than 10 μmol/L was recognized as safe for hepatocytes in vitro.

• MeSH
• čaj chemie   metabolismus   MeSH
• fluorescenční mikroskopie MeSH
• hepatocyty cytologie   účinky léků   metabolismus   MeSH
• jaterní mitochondrie účinky léků   metabolismus   MeSH
• kaspasa 3 metabolismus   MeSH
• katechin analogy a deriváty   toxicita   MeSH
• krysa rodu Rattus MeSH
• kultivované buňky MeSH
• membránový potenciál mitochondrií účinky léků   MeSH
• oxidativní fosforylace účinky léků   MeSH
• potkani Wistar MeSH
• reaktivní formy kyslíku metabolismus   MeSH
• TNF-alfa metabolismus   MeSH
• viabilita buněk účinky léků   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• čaj MeSH
• epigallocatechin gallate MeSH Prohlížeč
• kaspasa 3 MeSH
• katechin MeSH
• reaktivní formy kyslíku MeSH
• TNF-alfa MeSH

Sulfur mustard (SM) is a blister agent with cytotoxic mechanism of action. There is no suitable treatment based on administration of an antidote. In this study, Wistar rats were exposed to SM in doses of 0-40 mg/kg body weight and treated with the compound HI-6. The treatment provided no significant effect on ferric reducing antioxidant power of blood and plasma. However, HI-6 caused an increase in the level of thiobarbituric acid reactive substances. This stressogenic response was presumably the cause of the significant elevation of the blood level of both glutathione reductase and reduced glutathione. HI-6 appears to be suitable for enhancing prophylactically oxidative stress protection from small oxidative insult.

• Klíčová slova
• acetylcholinesterase, blister agent, cholinergic anti-inflammatory pathway, oxidative insult, reactive oxygen species,
• Publikační typ
• časopisecké články MeSH

Oxidative stress plays a dual role in infections. Free radicals protect against invading microorganisms, and they can also cause tissue damage during the resulting inflammation. In the process of infection, there is generation of reactive species by myeloperoxidase, NADPH oxidase, and nitric oxide synthase. On the other hand, reactive species can be generated among others, by cytochrome P450, some metals, and xanthine oxidase. Some pathologies arising during infection can be attributed to oxidative stress and generation of reactive species in infection can even have fatal consequences. This article reviews the basic pathways in which reactive species can accumulate during infectious diseases and discusses the related health consequences.

• MeSH
• infekční nemoci imunologie   patofyziologie   MeSH
• kovy metabolismus   MeSH
• lidé MeSH
• metabolické sítě a dráhy MeSH
• NADPH-oxidasy metabolismus   MeSH
• oxidační stres * MeSH
• peroxidasa metabolismus   MeSH
• synthasa oxidu dusnatého metabolismus   MeSH
• systém (enzymů) cytochromů P-450 metabolismus   MeSH
• volné radikály imunologie   metabolismus   toxicita   MeSH
• xanthinoxidasa metabolismus   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH
• Názvy látek
• kovy MeSH
• NADPH-oxidasy MeSH
• peroxidasa MeSH
• synthasa oxidu dusnatého MeSH
• systém (enzymů) cytochromů P-450 MeSH
• volné radikály MeSH
• xanthinoxidasa MeSH